Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,121,445 A→G 9.4% N296D (AAC→GAC)  PP_0982 → YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,121,4450AG 9.4% 138.6 / 5.5 53N296D (AAC→GAC) PP_0982YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base G (3/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-01

GTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCT  >  NC_002947/1121304‑1121593
                                                                                                                                             |                                                                                                                                                    
gTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCcgcg                                                                                                                                               >  6:273005/1‑149 (MQ=255)
   aCCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTTGCGGTGCCGCTGTCGCGGGTCAACCCGCGcc                                                                                                                                             <  6:99707/148‑1 (MQ=255)
   aCCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGcc                                                                                                                                             <  1:229598/148‑1 (MQ=255)
        gcgcGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGcc                                                                                                                                       >  8:222331/1‑149 (MQ=255)
            gAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGTTGTTCGTCGTCACCCTGATGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCt                                                                                                                                    >  7:143197/1‑148 (MQ=255)
            gAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGA‑GGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                                  <  7:168118/149‑1 (MQ=255)
             aaGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                                  <  7:177987/149‑1 (MQ=255)
                cgcTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTc                                                                                                                               <  8:80108/149‑1 (MQ=255)
                      ccACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGCCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctg                                                                                                                         >  4:133793/1‑149 (MQ=255)
                        aCCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                       <  8:305937/149‑1 (MQ=255)
                        aCCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                       >  5:237256/1‑149 (MQ=255)
                             cGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCCGCGACAgc                                                                                                                                          <  6:95215/125‑2 (MQ=255)
                             cGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCCGCGACAgc                                                                                                                                          >  5:95215/1‑124 (MQ=255)
                             cGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCgg                                                                                                                  <  1:332312/149‑1 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                                  >  7:304316/1‑127 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                                  <  8:304316/127‑1 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                            >  7:173723/1‑149 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                            >  7:231920/1‑149 (MQ=255)
                                    aTCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATGCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                            <  2:1876/148‑1 (MQ=255)
                                     tCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttctt                                                                                                          <  5:273005/149‑1 (MQ=255)
                                        gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAg                                                                                                                            >  1:283612/1‑128 (MQ=255)
                                        gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAg                                                                                                                            <  2:283612/128‑1 (MQ=255)
                                        gCAAGGAGGGCCTGCGTGAGAGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTg                                                                                                       >  2:98053/1‑149 (MQ=255)
                                          aaGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTa                                                                                                     <  2:171/149‑1 (MQ=255)
                                             gaggGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                        >  5:259241/1‑143 (MQ=255)
                                             gaggGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                        <  6:259241/143‑1 (MQ=255)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGcc                                                                                              >  8:221549/1‑148 (MQ=255)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                             <  8:231920/149‑1 (MQ=255)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCCGCGACAg                                                                                                                                           <  7:103647/103‑1 (MQ=39)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCCGCGACAg                                                                                                                                           >  8:103647/1‑103 (MQ=39)
                                                      cGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCt                                                                                         <  8:173723/149‑1 (MQ=255)
                                                               gcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCt                                                                                <  7:221549/149‑1 (MQ=255)
                                                                     cTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTc                                                                          <  4:12784/149‑1 (MQ=255)
                                                                             gCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTAcgcg                                                                  >  2:121976/1‑149 (MQ=255)
                                                                              cGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACgcgg                                                                 <  7:222331/149‑1 (MQ=255)
                                                                                   gTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACgcggctca                                                             <  1:98053/148‑4 (MQ=255)
                                                                                          ccGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCgag                                                       >  7:123220/1‑147 (MQ=255)
                                                                                           cGATCCTGGTGTTCGTCGTCACCCTGCTGGAGGTGCCGATGTCGCGGGTCAACCCGCTCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAg                                                    <  8:143197/149‑1 (MQ=255)
                                                                                                          tcgtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATc                                     >  1:153804/1‑149 (MQ=255)
                                                                                                            gtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGc                                   >  6:230436/1‑149 (MQ=255)
                                                                                                                 ccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTgg                              <  3:133793/149‑1 (MQ=255)
                                                                                                                 ccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTgg                              <  2:153804/149‑1 (MQ=255)
                                                                                                                     ctgGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAt                          >  7:325856/1‑149 (MQ=255)
                                                                                                                         cGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCCAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtgg                      <  8:123220/149‑1 (MQ=255)
                                                                                                                          ggTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtggt                     <  1:121976/149‑1 (MQ=255)
                                                                                                                            tGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGtggtgg                   <  5:230436/149‑1 (MQ=255)
                                                                                                                                gCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTc                >  5:362336/1‑148 (MQ=255)
                                                                                                                                  tggcgcgGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCAc             >  1:33271/4‑149 (MQ=255)
                                                                                                                                      gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGc                            >  6:97053/1‑130 (MQ=255)
                                                                                                                                      gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGc                            <  5:97053/130‑1 (MQ=255)
                                                                                                                                      gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGcc         >  1:36391/1‑149 (MQ=255)
                                                                                                                                       cgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCt        >  8:203440/1‑149 (MQ=255)
                                                                                                                                          gTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGtt     >  6:28287/1‑149 (MQ=255)
                                                                                                                                             aaCCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCct  <  1:71900/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTTCCT  >  NC_002947/1121304‑1121593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑