Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,140,505	G→A	7.5%	L155L (CTG→TTG)	arcA ←	arginine deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,140,505	0	G	A	7.5%	153.7 / 2.1	53	L155L (CTG→TTG)	arcA	arginine deiminase
Reads supporting (aligned to +/- strand):  ref base G (28/21);  new base A (2/2);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

GTGAACTCCTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCTTCGAGCCAGCTACGCA  >  NC_002947/1140360‑1140648                                                                                                                                                  |                                                                                                                                                 gTGAACTCCTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGACGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGAt                                                                                                                                               >  3:244034/1‑149 (MQ=255)    aaCTCCTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATg                                                                                                                                              <  6:217971/147‑1 (MQ=255)    aaCTCCTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATg                                                                                                                                              >  5:217971/1‑147 (MQ=255)       tCCTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAAGATGAAGCt                                                                                                                                         >  1:12566/1‑149 (MQ=255)       tCCTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAAGATGAAGCt                                                                                                                                         >  3:113029/1‑149 (MQ=255)         cTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACTCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTg                                                                                                                                        >  3:219981/1‑148 (MQ=255)         cTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTg                                                                                                                                        >  7:240234/1‑148 (MQ=255)            gtgGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGa                                                                                                                                      <  2:102539/147‑1 (MQ=255)                   ttGTAGATGGCGGTGGTCAAAAGCGTTTCCTGCCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGGGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAg                                                                                                                             >  2:300619/1‑149 (MQ=255)                     gTAGATGGCGGTGGTCAAAAGCGTGTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAg                                                                                                                             <  6:233958/147‑1 (MQ=255)                        gATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAg                                                                                                                         <  8:240234/148‑1 (MQ=255)                         aTGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTg                                                                                                                                        <  1:226041/132‑1 (MQ=255)                         aTGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTg                                                                                                                                        >  2:226041/1‑132 (MQ=255)                                    cAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCt                                                                                                            >  4:145473/1‑149 (MQ=255)                                       aaGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCa                                                                                                         >  6:38992/1‑149 (MQ=255)                                        aGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCAc                                                                                                        <  5:38992/149‑1 (MQ=255)                                           gTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGa                                                                                                      >  3:59696/1‑148 (MQ=255)                                              tCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTg                                                                                                                   >  4:339536/1‑132 (MQ=255)                                              tCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTg                                                                                                                   <  3:339536/132‑1 (MQ=255)                                                   gCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGgcgc                                                                                             <  3:145473/149‑1 (MQ=255)                                                    cGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGcc                                                                                            >  3:272432/1‑149 (MQ=255)                                                     gACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCt                                                                                           >  2:98214/1‑149 (MQ=255)                                                         cgcCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGc                                                                                       <  7:252671/149‑1 (MQ=255)                                                              gCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGc                                                                                  <  8:300084/149‑1 (MQ=255)                                                                 aGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCgg                                                                                >  7:103338/1‑148 (MQ=255)                                                                 aGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCgg                                                                                >  5:138806/1‑148 (MQ=255)                                                                      aTCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTc                                                                          >  1:316957/1‑149 (MQ=255)                                                                       tCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTcc                                                                         >  3:60102/1‑149 (MQ=255)                                                                              cAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGccc                                                                  <  4:244034/148‑1 (MQ=255)                                                                              cAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAAGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGcgc                                                                  <  2:12566/148‑3 (MQ=255)                                                                              cAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAAGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGccc                                                                  <  4:113029/148‑1 (MQ=255)                                                                               aaCGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGc                                                                >  4:248178/1‑149 (MQ=255)                                                                                aCGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGc                                                                >  7:67924/1‑148 (MQ=255)                                                                                     aCGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTGGTTGTACATCTTCATGACATCGGCGCCTTCGCTTGGCGGCCGGTGCT‑Ggcgcgcacggcc                                                          >  8:293254/1‑143 (MQ=255)                                                                                      cgccgcCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCAcgccg                                                         >  5:86058/1‑149 (MQ=255)                                                                                                   ccAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTAc                                                                                                                >  1:275/1‑82 (MQ=255)                                                                                                   ccAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTAc                                                                                                                <  2:275/82‑1 (MQ=255)                                                                                                           tggtgTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAgg                                    >  2:132772/1‑149 (MQ=255)                                                                                                                tCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGgtggc                                <  1:300619/148‑1 (MQ=255)                                                                                                                   cgcgTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGgtggcgtgg                            >  3:233021/1‑149 (MQ=255)                                                                                                                      gTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTc                         <  4:60102/149‑1 (MQ=255)                                                                                                                         aaCTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAg                      <  6:86058/149‑1 (MQ=255)                                                                                                                         aaCTGGGTGTGGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCATGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCa                       <  7:293254/148‑1 (MQ=255)                                                                                                                            tGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGcc                   >  3:158207/1‑149 (MQ=255)                                                                                                                            tGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGcc                   >  3:69685/1‑149 (MQ=255)                                                                                                                             gggTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCt                  <  4:59696/149‑1 (MQ=255)                                                                                                                             gggTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCt                  >  2:252770/1‑149 (MQ=255)                                                                                                                              ggTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGcc                   >  1:60542/1‑147 (MQ=255)                                                                                                                              ggTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGcc                   <  2:60542/147‑1 (MQ=255)                                                                                                                                  ttGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCTTCGAg             <  8:103338/149‑1 (MQ=255)                                                                                                                                  ttGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCTTCGAg             <  6:138806/149‑1 (MQ=255)                                                                                                                                   tGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCTAGGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCTTCGa              <  1:98214/148‑1 (MQ=255)                                                                                                                                             tggCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCTTCGAGCCAGCTACGCa  >  7:141395/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 GTGAACTCCTTGTGGAACTTGTAGATGGCGGTGGTCAAAAGCGTTTCCTGGCGACGCGCCGGCCAGTACATCGGGTTCAACGTCACGCCGCCGTAGATCCAGCAGGTGGTGTCGCGCGTGAACTGGGTGTTGGGCAGTGGTGGCAGGATGAAGCTGGAGTGGCCCAGGTAGTCGTTGTACATCTTCACGACATCGGCGCCTTCGCTTTGCGGCAGGTCCT‑GGCCCGCCACGCCGCCGATCAGGAATTCGGCCAGGTGGCGTGGCTCCAGGCCTTCGAGCCAGCTACGCA  >  NC_002947/1140360‑1140648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑