Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,155,067 G→A 7.0% V75V (GTG→GTA)  PP_1013 → sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,155,0670GA 7.0% 213.2 / 3.3 71V75V (GTG→GTA) PP_1013sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (34/32);  new base A (3/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCTGCTGGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGATGTGTTTCAG  >  NC_002947/1154927‑1155205
                                                                                                                                            |                                                                                                                                          
gTCCTGCTGGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTAGGCCACCg                                                                                                                                    >  6:70435/1‑149 (MQ=255)
 tCCTGCTGGCCCAGGGGTTGTCCGGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCGGCCGGTAGGCCACCgg                                                                                                                                   >  7:329852/1‑149 (MQ=255)
 tCCTGCTGGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTgg                                                                                                                                           <  2:82056/141‑1 (MQ=255)
 tCCTGCTGGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTgg                                                                                                                                           >  1:82056/1‑141 (MQ=255)
   ctgctgGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTAGGCCACCgg                                                                                                                                   >  2:185633/1‑147 (MQ=255)
      ctgGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCt                                                                                                                              >  7:168561/1‑149 (MQ=255)
      ctgGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTAGGCCACCGGcag                                                                                                                                <  1:185633/147‑3 (MQ=255)
      ctgGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTAGGCCACCGGcag                                                                                                                                <  5:70435/147‑3 (MQ=255)
         gCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCTCCAGCCAGCATCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTAGTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTgg                                                                                                                            <  3:119157/148‑1 (MQ=255)
           ccAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGc                                                                                                                         >  4:142406/1‑149 (MQ=255)
                       cAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTg                                                                                                              <  4:293800/148‑1 (MQ=255)
                        aGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTgcg                                                                                                            <  6:4464/149‑1 (MQ=255)
                          cATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTgcg                                                                                                            >  2:166230/1‑147 (MQ=255)
                          cATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCgca                                                                                                          <  1:166230/149‑1 (MQ=255)
                              aTCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCAt                                                                                                      >  3:348920/1‑149 (MQ=255)
                                   ggTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGggcg                                                                                                 <  2:297160/149‑1 (MQ=255)
                                   ggTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGggcg                                                                                                 >  2:97416/1‑149 (MQ=255)
                                         tcAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCAcgc                                                                                           >  5:283888/1‑149 (MQ=255)
                                           aGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGt                                                                                         >  8:253029/1‑149 (MQ=255)
                                           aGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGt                                                                                         >  7:200896/1‑149 (MQ=255)
                                             cTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGttt                                                                                       <  3:142406/149‑1 (MQ=255)
                                                 gcgcCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTttcttc                                                                                   <  8:168561/149‑1 (MQ=255)
                                                   gccagccaGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGt                                                                                         <  1:335778/141‑1 (MQ=255)
                                                   gccagccaGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGt                                                                                         >  2:335778/1‑141 (MQ=255)
                                                   gccagccaGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCgt                                                                                 <  6:196531/149‑1 (MQ=255)
                                                        ccagcTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGtt                                                                                        >  1:92058/1‑137 (MQ=255)
                                                        ccagcTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGtt                                                                                        <  2:92058/137‑1 (MQ=255)
                                                        ccagcTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGc                                                                            <  7:253029/149‑1 (MQ=255)
                                                            cTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCaa                                                                        <  2:146918/149‑1 (MQ=255)
                                                                 ggggCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAacgcca                                                                   >  3:124177/1‑149 (MQ=255)
                                                                 ggggCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAacgcca                                                                   <  6:283888/149‑1 (MQ=255)
                                                                   ggCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAacgccacg                                                                 >  4:152025/1‑149 (MQ=255)
                                                                      tGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCAcgccg                                                              >  7:190701/1‑149 (MQ=255)
                                                                       gCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCAcgccgc                                                             >  1:15914/1‑149 (MQ=255)
                                                                        cGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTttctt                                                                                    <  6:45333/125‑1 (MQ=255)
                                                                        cGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTttctt                                                                                    >  5:45333/1‑125 (MQ=255)
                                                                            ccagcgccagcAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGa                                                         >  7:323368/1‑148 (MQ=255)
                                                                            ccagcgccagcAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGAc                                                        <  4:348920/149‑1 (MQ=255)
                                                                             catgtccagcAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACa                                                       <  2:200083/144‑1 (MQ=255)
                                                                                cgccagcAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGc                                                    <  8:323368/149‑1 (MQ=255)
                                                                                        gcCAGGCGCATTCCATGAGTGTCAGCGTCAGTTACTTCCGCTCCCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGGGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAAAGCCCCGCCGCTGGACATGCCGGCACTg                                            >  6:270222/1‑149 (MQ=255)
                                                                                         ccTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGc                                           >  8:5618/1‑149 (MQ=255)
                                                                                               gcATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACGTGCAGGCACTGCCCGTCa                                     >  1:228457/1‑149 (MQ=255)
                                                                                                 aTTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGt                                       <  5:349276/145‑1 (MQ=255)
                                                                                                 aTTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGt                                       >  6:349276/1‑145 (MQ=255)
                                                                                                  ttCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCAccc                                  >  8:140798/1‑149 (MQ=255)
                                                                                                     cATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATg                                                     <  5:273427/127‑1 (MQ=255)
                                                                                                     cATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATg                                                     >  6:273427/1‑127 (MQ=255)
                                                                                                     cATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTc                               <  8:200896/149‑1 (MQ=255)
                                                                                                     cATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTc                               >  7:104938/1‑149 (MQ=255)
                                                                                                      aTGAGTGCCAGCGTTAGTTACTTCCGCTCGCTGCCGGTGGCATAGCGTCCGCTGGTTCTCGACCAATTGCGCAGGATGGGCGGAACTCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACTTGCAGGCACTGCCCGTCAgccc                                 <  6:233539/146‑1 (MQ=255)
                                                                                                      aTGAGTGCCAGCGTCAGTAACTTCCGCTCGCTTACGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTTCGCAGCATGGTCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTCGACATGCAGGAACTTCCCGTCAccca                                 >  5:233539/1‑145 (MQ=255)
                                                                                                         aGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCaa                           <  1:97416/149‑1 (MQ=255)
                                                                                                         aGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCaa                           <  8:190701/149‑1 (MQ=255)
                                                                                                            gCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGa                                                         >  2:135440/1‑116 (MQ=255)
                                                                                                            gCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGa                                                         <  1:135440/116‑1 (MQ=255)
                                                                                                             ccAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAg                       >  8:190242/1‑149 (MQ=255)
                                                                                                              cAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCgt                                                                                 >  1:164993/1‑90 (MQ=255)
                                                                                                              cAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCgt                                                                                 <  2:164993/90‑1 (MQ=255)
                                                                                                              cAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAgg                      >  1:321502/1‑149 (MQ=255)
                                                                                                              cAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCAGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAgg                      >  2:99378/1‑149 (MQ=255)
                                                                                                                gCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCg                    <  2:15914/149‑1 (MQ=255)
                                                                                                                 cGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCgg                   <  4:144293/149‑1 (MQ=255)
                                                                                                                       ttACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATc              >  6:309141/1‑148 (MQ=255)
                                                                                                                             ccGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGAtgtg        <  3:265172/148‑1 (MQ=255)
                                                                                                                             ccGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGAtgt         <  3:152025/147‑1 (MQ=255)
                                                                                                                              cgctcgctGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGATGTGtt      >  1:36521/1‑149 (MQ=255)
                                                                                                                              cgctcgctGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCAGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCTGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGATgtgt       <  1:99378/148‑1 (MQ=255)
                                                                                                                               gctcgctGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACGTGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGATGTGttt     <  2:228457/149‑1 (MQ=255)
                                                                                                                                  cgctGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCt                                                                           >  7:27408/1‑76 (MQ=255)
                                                                                                                                   gctGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCACCGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGATGTGTTTcag  >  5:363801/1‑148 (MQ=255)
                                                                                                                                         ggTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGAtgt         >  8:188798/1‑135 (MQ=255)
                                                                                                                                         ggTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGAtgt         <  7:188798/135‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GTCCTGCTGGCCCAGGGGTTGTCCAGCATTATCTGGGTATCTCAGCTGCGCGCCAGCCAGCTTCAGGGGCTGCGTGCCAGCGCCAGCAGCCTGGCGCATTCCATGAGTGCCAGCGTCAGTTACTTCCGCTCGCTGCCGGTGGCCTACCGGCCGCTGGTGCTCGACCAATTGCGCAGCATGGGCGGCACGCGTTTCTTCGTGTCGCTCAACGCCACGCCGCTGGACATGCAGGCACTGCCCGTCACCCCTCGCAAGCAGGCGGTGATCGATGTGTTTCAG  >  NC_002947/1154927‑1155205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑