Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,178,808 G→A 8.5% A657V (GCC→GTC)  PP_1033 ← sulfatase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,178,8080GA 8.5% 130.9 / 2.2 47A657V (GCC→GTC) PP_1033sulfatase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base A (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

CAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTAGTCAGGGTGTCCGAACTGAATTCGTATAT  >  NC_002947/1178665‑1178956
                                                                                                                                                 |                                                                                                                                                    
cAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAAt                                                                                                                                                 <  1:187224/149‑1 (MQ=255)
        cTGCCACTATCGAGTGGGAGTGATCGGGTAGCGCAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCtt                                                                                                                                          >  1:223700/1‑149 (MQ=255)
         tcccACTATCGAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATa                                                                                                                                                <  1:170415/139‑1 (MQ=255)
         tcccACTATCGAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATa                                                                                                                                                >  2:170415/3‑141 (MQ=255)
              cTATCGAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCGTCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGACAAATAGCGCTTTCTGGt                                                                                                                                    >  1:74640/1‑149 (MQ=255)
              cTATCGAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTa                                                                                                                                   <  3:169139/149‑1 (MQ=255)
                   gAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCg                                                                                                                               <  6:242612/148‑1 (MQ=255)
                         gAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAgg                                                                                                                        <  6:226740/149‑1 (MQ=255)
                            tGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGa                                                                                                                       >  1:103731/1‑147 (MQ=255)
                             gATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTAGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGa                                                                                                                       >  8:329499/1‑146 (MQ=255)
                               tCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAagca                                                                                                                  <  2:223700/149‑1 (MQ=255)
                                           gccCAGGCTGGCACCCG‑CAGGACGGATACACCAGAACGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGctcctc                                                                                                       <  7:224983/149‑1 (MQ=255)
                                             ccAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCAc                                                                                                     >  7:313189/1‑149 (MQ=255)
                                             ccAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCAc                                                                                                     >  5:42709/1‑149 (MQ=255)
                                                  cTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGa                                                                                                <  3:33571/149‑1 (MQ=255)
                                                       aCCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTTTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTg                                                                                                   >  1:315109/1‑141 (MQ=255)
                                                       aCCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTTTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTg                                                                                                   <  2:315109/141‑1 (MQ=255)
                                                             cAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTc                                                                                      <  8:313189/149‑1 (MQ=255)
                                                               ggACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGACAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGt                                                                                       <  1:19857/146‑1 (MQ=255)
                                                               ggACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGACAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGt                                                                                       >  2:19857/1‑146 (MQ=255)
                                                                        cacCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcgc                                                                           >  7:216880/1‑149 (MQ=255)
                                                                        cacCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGACAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcgc                                                                           <  2:74640/149‑1 (MQ=255)
                                                                         acCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGATCGTTCAGATTGCTTATTCTTCTGGCTTACTGGCAAATAGCGCTTTCTGGTAGGCGGTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCAcgcg                                                                          >  5:351115/1‑149 (MQ=255)
                                                                                aGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTgggg                                                                    >  5:35937/1‑148 (MQ=255)
                                                                                aGCCAGCATGTTTGCTGGCTTTCTGGTTCTTGTTAGTTCTGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTATGCGTTATAGGTAAGCAGCCTGCTCCTCACTTCTATGAACTCGTCACCCTCCACGCTTTgggg                                                                    <  6:35937/148‑1 (MQ=255)
                                                                                  cccGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTg                                                                 <  4:235194/149‑1 (MQ=255)
                                                                                           ttGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCCCAACGCGTGGAGGGTgacga                                                             <  2:282437/144‑5 (MQ=255)
                                                                                           ttGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTGATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCCCAACGCGTGGAGGGTgacga                                                             >  1:282437/1‑140 (MQ=255)
                                                                                              cGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCgg                                                     >  3:242502/1‑149 (MQ=255)
                                                                                              cGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCg                                                      <  6:42709/148‑1 (MQ=255)
                                                                                               gggCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCggg                                                    <  2:103731/149‑1 (MQ=255)
                                                                                                     tCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATc                                               <  5:152477/148‑1 (MQ=255)
                                                                                                            cTTGTTCTTTCAGATTGCTTATTCTTCTGGCTTTCTGGGAATTAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCATGCTGCTCTCTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAgg                                        <  6:351115/148‑1 (MQ=255)
                                                                                                                    ttcAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTAtt                                 >  2:16020/1‑147 (MQ=255)
                                                                                                                      cAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTACTCGCGTTGGGGGTGCTTTTCCTGCTGGTCATCCTCAAGGTTGTATTTAg                              <  7:329499/148‑1 (MQ=255)
                                                                                                                        gATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCt                                             <  1:156525/131‑1 (MQ=255)
                                                                                                                        gATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCt                                             >  2:156525/1‑131 (MQ=255)
                                                                                                                          ttGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCAGCGGGTCATCCTCAAGGTTGTATTTAGTCAgg                         <  8:216880/149‑1 (MQ=255)
                                                                                                                              ttATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAg                                         <  6:345780/129‑1 (MQ=255)
                                                                                                                              ttATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAg                                         >  5:345780/1‑129 (MQ=255)
                                                                                                                              ttATTCTTCTGGCTTTCTGGAAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTAGTCAGGgtgt                     >  2:239441/1‑149 (MQ=255)
                                                                                                                                  tcttctGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTa                               <  7:192436/135‑1 (MQ=255)
                                                                                                                                  tcttctGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTa                               >  8:192436/1‑135 (MQ=255)
                                                                                                                                        ggCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGGTTTTCATGCGGGGCATCCTCAAGGTTGTATTTAGTCAGGGTGTCCGACGTGaa           >  4:76238/1‑149 (MQ=255)
                                                                                                                                         gCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTAGTCAGGGTGTCCGAACTGAAt          >  6:273380/1‑149 (MQ=255)
                                                                                                                                               tGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTAGTCAGGGTGTCCGAACTGa            >  7:66911/1‑141 (MQ=255)
                                                                                                                                               tGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTAGTCAGGGTGTCCGAACTGa            <  8:66911/141‑1 (MQ=255)
                                                                                                                                                 gCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTAGTCAGGGTGTCCGAACTGAATTCGTatat  >  4:203037/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAAGCCGCCTGCCACTATCGAGTGGGAGTGATCGGGTAGC‑CAGCCCAGGCTGGCACCCG‑CAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCCTCACTGCGATGAACTCGTCACCCTCCACGCGTTGGGGGTGCTTTTCCTGCGGGTCATCCTCAAGGTTGTATTTAGTCAGGGTGTCCGAACTGAATTCGTATAT  >  NC_002947/1178665‑1178956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑