Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,210,131	T→A	6.4%	I247N (ATC→AAC)	ytnA →	amino acid permease YtnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,210,131	0	T	A	6.4%	190.4 / 2.1	62	I247N (ATC→AAC)	ytnA	amino acid permease YtnA
Reads supporting (aligned to +/- strand):  ref base T (28/30);  new base A (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CAACGGCGGTTTCATGCCCAATGGTGTGACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCATCAAGACCGCTGCCGGC  >  NC_002947/1209991‑1210276                                                                                                                                             |                                                                                                                                                   cAACGGCGGTTTCATGCCCAATGGTGTGACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCgg                                                                                                                                            >  1:50981/1‑149 (MQ=255)   aCGGCGGTTTCATGCCCAATGGTGTGACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGgtg                                                                                                                                          >  1:323776/1‑149 (MQ=255)      gcggTTTCATGCCCAATGGTGTGACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTc                                                                                                                                       >  6:69017/1‑149 (MQ=255)              aTGCCCAATGGTGTGACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCAt                                                                                                                               >  5:210360/1‑149 (MQ=255)                    aaTGGTGTGACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCc‑tgct                                                                                                                        >  3:128253/1‑149 (MQ=255)                         tgtgACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCt                                                                                                                   <  4:293031/149‑1 (MQ=255)                                 gCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGgc                                                                                                           >  8:110816/1‑149 (MQ=255)                                 gCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGgc                                                                                                           <  6:210360/149‑1 (MQ=255)                                       tGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCgttg                                                                                                     >  6:132712/1‑149 (MQ=255)                                         aTGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCgttgtt                                                                                                   <  2:50981/149‑1 (MQ=255)                                          tGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTc                                                                                                  <  5:132712/149‑1 (MQ=255)                                            tCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGgcgc                                                                                                         >  5:10603/1‑140 (MQ=255)                                            tCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGgcgc                                                                                                         <  6:10603/140‑1 (MQ=255)                                            tCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCg                                                                                                 <  3:78806/148‑1 (MQ=255)                                             cGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGt                                                                                                >  3:18034/1‑148 (MQ=255)                                              gCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGACGGCGCGTTGTTCGTGa                                                                                              <  3:24923/149‑1 (MQ=255)                                                  cAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATc                                                                                            <  5:69017/147‑1 (MQ=255)                                                     aTGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTc                                                                                       >  1:275903/1‑149 (MQ=255)                                                       ggTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGa                                                                                     >  4:13946/1‑149 (MQ=255)                                                        gTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGa                                                                                     <  2:56656/148‑1 (MQ=255)                                                        gTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGa                                                                                     <  3:191149/148‑1 (MQ=255)                                                             gTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGAt                                                                                    >  3:200474/1‑144 (MQ=255)                                                             gTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGAt                                                                                    <  4:200474/144‑1 (MQ=255)                                                                 gCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTg                                                                           >  3:31942/1‑149 (MQ=255)                                                                 gCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTg                                                                           <  4:18034/149‑1 (MQ=255)                                                                      tCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGaa                                                                        <  7:110816/147‑1 (MQ=255)                                                                      tCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAAc                                                                       <  5:209951/148‑1 (MQ=255)                                                                       cTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACg                                                                      >  7:22510/1‑148 (MQ=255)                                                                                gAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGaaa                                                                   <  7:339488/142‑1 (MQ=255)                                                                                gAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGaaa                                                                   >  8:339488/1‑142 (MQ=255)                                                                                gAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTa                                                             <  3:27028/148‑1 (MQ=255)                                                                                gAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTa                                                             <  2:323776/148‑1 (MQ=255)                                                                                  aaTGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGc                                                           >  1:227175/1‑148 (MQ=255)                                                                                      aTCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAggg                                                      <  4:31942/149‑1 (MQ=255)                                                                                      aTCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAggg                                                      <  2:275903/149‑1 (MQ=255)                                                                                            cTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGc                                                  >  6:265344/1‑147 (MQ=255)                                                                                                ccgccgGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAggg                                                      <  8:157253/139‑1 (MQ=255)                                                                                                ccgccgGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTc                                            <  4:128253/149‑1 (MQ=255)                                                                                                ccgccgGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCA‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAggg                                                      >  7:157253/1‑139 (MQ=255)                                                                                                  gccgGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGt                                          >  8:247014/1‑149 (MQ=255)                                                                                                     ggTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCTGGGCAGCCCGTTCGTCAt                                       <  3:13946/149‑1 (MQ=255)                                                                                                      gTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCAt                                       >  2:120998/1‑148 (MQ=255)                                                                                                       tGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGa                                     <  8:22510/149‑1 (MQ=255)                                                                                                         aaGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGAcc                                   >  5:3044/1‑149 (MQ=255)                                                                                                             aCGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTc                                >  1:73772/1‑148 (MQ=255)                                                                                                             aCGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCg                               <  2:73772/149‑1 (MQ=255)                                                                                                               gCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAg                             <  7:247014/149‑1 (MQ=255)                                                                                                                 aaCCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAggg                                                      >  6:201628/1‑122 (MQ=255)                                                                                                                 aaCCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAggg                                                      <  5:201628/122‑1 (MQ=255)                                                                                                                 aaCCCGCAAAAGACCATTCCGCAGGCCAACGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAgcg                           >  2:30707/1‑149 (MQ=255)                                                                                                                 aaCCCGCAAAAGACCATTCCGCAGGCCAACGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAgcg                           >  2:25196/1‑149 (MQ=255)                                                                                                                       cAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGc                                                  <  1:197068/120‑1 (MQ=255)                                                                                                                       cAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGc                                                  >  2:197068/1‑120 (MQ=255)                                                                                                                          aaGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCGGTCGATTTACCCGTGGAAGGAGAACGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCAt                  >  6:267315/1‑149 (MQ=255)                                                                                                                          aaGACCATTCCGCAGGCCAACGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCNGTTCGTCATGACCTTCGAGCGCCTCGGCAt                  <  1:30707/149‑1 (MQ=255)                                                                                                                          aaGACCATTCCGCAGGCCAACGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCAt                  <  1:25196/149‑1 (MQ=255)                                                                                                                             aCCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTa                                                             >  3:173814/1‑103 (MQ=255)                                                                                                                             aCCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTa                                                             <  4:173814/103‑1 (MQ=255)                                                                                                                              ccATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCATc                 >  4:316622/1‑146 (MQ=255)                                                                                                                                   ccGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCATCAAGACCg          <  6:3044/148‑1 (MQ=255)                                                                                                                                    cGCAGGCCATCGGCTCGGTGTTCTGGCGCATCCGTGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCATCAAGACCGc         >  1:229474/1‑149 (MQ=255)                                                                                                                                          ccATCGGCGCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCATCAAGACCGCTGCCGGc  <  2:229474/149‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                   CAACGGCGGTTTCATGCCCAATGGTGTGACCGGCGTGCTGATGTCGCTGCAGATGGTGATGTTCGCCTATCTGGGTGTGGAAATGATCGGCCTCACCGCCGGTGAAGCACGCAACCCGCAAAAGACCATTCCGCAGGCCATCGGCTCGGTGTTCTGGCGCATCC‑TGCTGTTCTACGTCGGCGCGTTGTTCGTGATCCTGTCGATTTACCCGTGGAACGAAATCGGTAGCCAGGGCAGCCCGTTCGTCATGACCTTCGAGCGCCTCGGCATCAAGACCGCTGCCGGC  >  NC_002947/1209991‑1210276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑