Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,273,747	T→A	6.3%	R113R (CGA→CGT)	PP_1115 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,273,747	0	T	A	6.3%	192.9 / 2.2	63	R113R (CGA→CGT)	PP_1115	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (32/27);  new base A (2/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGAC  >  NC_002947/1273605‑1273893                                                                                                                                                |                                                                                                                                                   ggATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCg                                                                                                                                              >  4:30159/1‑149 (MQ=255)     tATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTc                                                                                                                                                   >  7:201672/1‑140 (MQ=255)     tATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTc                                                                                                                                                   <  8:201672/140‑1 (MQ=255)          aacaacTGAGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTAGCCCTTCAGGGAGTAACGCAGCGGCTGCTTGATTCTGAGCAGCTTGGCCAGTTGCTTAAGGTGCGTCCAA‑AGGTTGGGTCGTGCGGTGATCtt                                                                                                                                      >  2:194129/5‑148 (MQ=255)             cacTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGAt                                                                                                                                         <  8:304870/142‑1 (MQ=255)             cacTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGAt                                                                                                                                         >  7:304870/1‑142 (MQ=255)             cacTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGa                                                                                                                                    >  2:349763/1‑147 (MQ=255)             cacTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCCGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGaaa                                                                                                                                  <  1:72466/149‑1 (MQ=255)                  cgcgAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGGGGTGATCTTGAAAGTgcg                                                                                                                             >  4:217183/1‑149 (MQ=255)                  cgcgAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTgcg                                                                                                                             <  6:240986/149‑1 (MQ=255)                  cgcgAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTgcg                                                                                                                             <  1:349763/149‑1 (MQ=255)                     gagaTGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGGTTGGCCAGTGGGTTCAGGTGGGGCCAC‑AGGTTGGGTCGGGCGGGGATGGTGAACGGGCGCCg                                                                                                                          >  8:224964/1‑149 (MQ=255)                      agaTGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGt                                                                                                                         <  3:30159/149‑1 (MQ=255)                             cAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgcc                                                                                                                  >  4:174674/1‑149 (MQ=255)                                    cGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTACGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCAc                                                                                                           >  5:313229/1‑149 (MQ=255)                                     gatggatgAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGcc                                                                                                                           >  4:8590/1‑132 (MQ=255)                                     gatggatgAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGcc                                                                                                                           <  3:8590/132‑1 (MQ=255)                                               cAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgc                                                                                                                   >  8:13413/1‑130 (MQ=255)                                               cAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATgc                                                                                                                   <  7:13413/130‑1 (MQ=255)                                                    cGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCtt                                                                                                                                      <  2:269863/106‑1 (MQ=255)                                                    cGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCtt                                                                                                                                      >  1:269863/1‑106 (MQ=255)                                                       tCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCt                                                                                        <  2:279401/149‑1 (MQ=255)                                                       tCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCt                                                                                        >  6:222955/1‑149 (MQ=255)                                                       tCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCt                                                                                        <  3:174674/149‑1 (MQ=255)                                                         gCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCtt                                                                                       >  1:146118/1‑148 (MQ=255)                                                         gCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCGGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTc                                                                                      <  5:188650/149‑1 (MQ=255)                                                          ccAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCa                                                                                     >  6:75249/1‑149 (MQ=255)                                                          ccAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCa                                                                                     <  2:146118/149‑1 (MQ=255)                                                          ccAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTACGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCa                                                                                     >  6:296686/1‑149 (MQ=255)                                                           cAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCAt                                                                                    >  8:114594/1‑149 (MQ=255)                                                                   ccTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGccc                                                                             >  7:229806/1‑146 (MQ=255)                                                                         gggAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCATGCATGCCCCCCCACGCTGCGCCATCCGCTGGCTTCATCCTGCACCAGTAGc                                                                      >  5:25730/1‑149 (MQ=255)                                                                            aGTAACGCAGCGGCTGCTTGCTTCTGAGCAACTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAgg                                                                   >  1:32006/1‑149 (MQ=255)                                                                                 cgccgcGGCTGCTTGCTTCTGAGCCGCTTGGCCAGTGGCGTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCAtt                                                              <  7:224964/149‑1 (MQ=255)                                                                                 cGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCAt                                                                                                 >  6:44790/1‑114 (MQ=255)                                                                                 cGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCAt                                                                                                 <  5:44790/114‑1 (MQ=255)                                                                                  gCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCAtt                                                              >  5:220278/1‑148 (MQ=255)                                                                                  gCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTg                                                             <  5:75249/149‑1 (MQ=255)                                                                                    aGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTACGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAg                                                           <  6:313229/149‑1 (MQ=255)                                                                                         ctgctTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTa                                                      <  2:32006/149‑1 (MQ=255)                                                                                            cttgcttCTGAGCAGCTTTGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGAGCCGTTCATGCCCGCCCACGCTGCGCCATACGCTGGATTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTAtt                                                    <  1:194129/148‑1 (MQ=255)                                                                                            cttgctgCTGAGCAGCTTGGCCAGTGGCTGCAGGGGCTGCCAC‑AGGGTGGTTCGTGCGGTGATCGTGAAAGTGCGCCATGCATGCCCACCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTg                                                   <  6:25730/149‑1 (MQ=255)                                                                                             ttgcttCTGAGCAGCTTGGCCAGTGGCTTCTGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTg                                                   >  1:30405/1‑148 (MQ=255)                                                                                                 ttCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTTTTGCATAc                                              >  4:29235/1‑149 (MQ=255)                                                                                                 ttCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATAc                                              >  5:331953/1‑149 (MQ=255)                                                                                                        cagcTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAgg                                                          <  3:187642/130‑1 (MQ=255)                                                                                                        cagcTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAgg                                                          >  4:187642/1‑130 (MQ=255)                                                                                                          gcTTGGCCAGTGGCTTCAGGTGCTGCCACGAGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCAt                                                >  1:26949/1‑139 (MQ=255)                                                                                                          gcTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCAt                                                <  2:26949/138‑1 (MQ=255)                                                                                                           cTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACg                                        <  8:9076/145‑1 (MQ=255)                                                                                                           cTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACg                                        >  7:9076/1‑145 (MQ=255)                                                                                                              ggCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGaa                                 >  5:298138/1‑149 (MQ=255)                                                                                                               gCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAAt                                >  5:171933/1‑149 (MQ=255)                                                                                                                 cAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAAt                                <  7:5008/147‑1 (MQ=255)                                                                                                                 cAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAAt                                >  8:5008/1‑147 (MQ=255)                                                                                                                 cAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATg                               >  1:208573/1‑148 (MQ=255)                                                                                                                   gTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAAc                            <  2:30405/149‑1 (MQ=255)                                                                                                                   gTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAAc                            <  6:298138/149‑1 (MQ=255)                                                                                                                   gTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTACATACGACACGTTGCGAATGAAc                            <  8:229806/149‑1 (MQ=255)                                                                                                                         tCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAggcgg                      >  6:255767/1‑149 (MQ=255)                                                                                                                                    cac‑aGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTggg           <  7:114594/149‑1 (MQ=255)                                                                                                                                    cac‑aGGTTGGTACGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTggt           <  5:296686/149‑2 (MQ=255)                                                                                                                                        aGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGtt        >  3:198104/1‑149 (MQ=255)                                                                                                                                          gttggttCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGt      >  3:8054/1‑149 (MQ=255)                                                                                                                                              gttCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGAc  >  7:304995/1‑149 (MQ=255)                                                                                                                                              gttCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGAc  >  1:92083/1‑149 (MQ=255)                                                                                                                                              gttCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCAGCCCAAGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGACGCGTATAGCATACGACACGTTGAGAATGAAAAGGCGGATGTCATTGGGGTTGTCGAc  >  5:120523/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                   GGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGAGTAACGCAGCGGCTGCTTGCTTCTGAGCAGCTTGGCCAGTGGCTTCAGGTGCTGCCAC‑AGGTTGGTTCGTGCGGTGATCTTGAAAGTGCGCCGTGCATGCCCGCCCACGCTGCGCCATACGCTGGCTTCATCCTGCACCAGTAGCAGGTCATTGAGGCGTATTGCATACGACACGTTGCGAATGAACAGGCGGCTGTCATTGGGGTTGTCGAC  >  NC_002947/1273605‑1273893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑