Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,280,163 T→C 7.1% intergenic (‑168/‑439) PP_1117 ← / → PP_5742 hypothetical protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,280,1630TC 7.1% 158.0 / 2.5 56intergenic (‑168/‑439)PP_1117/PP_5742hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base C (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CTTGGGATAGTGTTAGGGCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTAAAGCGCACTGCAGGAG  >  NC_002947/1280017‑1280305
                                                                                                                                                  |                                                                                                                                              
cTTGGGATAGTGTTAGGGCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTtct                                                                                                                                              >  1:359136/1‑149 (MQ=255)
   gggATAGTGTTAGGGCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCa                                                                                                                                           >  7:189933/1‑149 (MQ=255)
           gtTAGGGCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGGGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTcc                                                                                                                                            >  1:253234/1‑140 (MQ=255)
           gtTAGGGCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGGGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTcc                                                                                                                                            <  2:253234/140‑1 (MQ=255)
            ttAGGGCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGAtgt                                                                                                                                  >  8:281636/1‑149 (MQ=255)
               gggCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGtt                                                                                                                               <  2:359136/149‑1 (MQ=255)
                     tgtAGCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAg                                                                                                                         <  1:1196/149‑1 (MQ=255)
                         gCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAAt                                                                                                                      <  7:281636/148‑1 (MQ=255)
                             cATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTAtt                                                                                                                 <  8:213524/149‑1 (MQ=255)
                                  tGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGAc                                                                                                             >  6:117406/1‑148 (MQ=255)
                                    gCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGa                                                                                                           >  5:269878/1‑148 (MQ=255)
                                               gtgtAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCaa                                                                                               <  8:189933/149‑1 (MQ=255)
                                                  tAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGc                                                                                             <  6:269878/148‑1 (MQ=255)
                                                      ggcgATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTtg                                                                                        <  7:30966/149‑1 (MQ=255)
                                                      ggcgATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTtg                                                                                        >  6:67540/1‑149 (MQ=255)
                                                       gcgATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTtgt                                                                                       >  4:195016/1‑149 (MQ=255)
                                                               cTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATGTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGcc                                                                               >  6:193763/1‑149 (MQ=255)
                                                                tACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATa                                                                                                         >  8:98394/1‑122 (MQ=255)
                                                                tACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATa                                                                                                         <  7:98394/122‑1 (MQ=255)
                                                                  cAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGt                                                                                                     >  3:96945/1‑124 (MQ=255)
                                                                  cAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGt                                                                                                     <  4:96945/124‑1 (MQ=255)
                                                                   aaaCTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGa                                                                                                                            <  8:13390/100‑1 (MQ=255)
                                                                   aaaCTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGa                                                                                                                            >  7:13390/1‑100 (MQ=255)
                                                                     aCTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTtctc                                                                                                                                             >  5:173084/1‑81 (MQ=255)
                                                                     aCTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTtctc                                                                                                                                             <  6:173084/81‑1 (MQ=255)
                                                                     aCTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTcaca                                                                          <  3:141924/148‑1 (MQ=255)
                                                                     aCTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTca                                                                            >  4:141924/1‑146 (MQ=255)
                                                                           tCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACCTGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTcacaagagc                                                                     <  3:195016/147‑6 (MQ=255)
                                                                             aaGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTc                                                                   >  7:197690/1‑147 (MQ=255)
                                                                               gCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAg                                                                >  6:345650/1‑148 (MQ=255)
                                                                                  gtgtTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGt                                                            >  3:203939/1‑149 (MQ=255)
                                                                                  gtgtTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGt                                                            >  3:334914/1‑149 (MQ=255)
                                                                                     ttGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATg                                                                                                                     >  1:104168/1‑89 (MQ=255)
                                                                                     ttGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATg                                                                                                                     <  2:104168/89‑1 (MQ=255)
                                                                                       ggCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGa                                                                                     <  8:158552/119‑1 (MQ=255)
                                                                                       ggCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGa                                                                                     >  7:158552/1‑119 (MQ=255)
                                                                                        gCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGgcg                                                                                 >  2:87721/1‑122 (MQ=255)
                                                                                        gCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGgcg                                                                                 <  1:87721/122‑1 (MQ=255)
                                                                                        gCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTgg                                                       <  4:334914/148‑1 (MQ=255)
                                                                                          aaGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCATGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGta                                                    >  3:52613/1‑149 (MQ=255)
                                                                                               ttGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTAtagta                                               <  6:53109/149‑1 (MQ=255)
                                                                                                   ccTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTATCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGTGTATAGTAGGGt                                           <  8:197690/149‑1 (MQ=255)
                                                                                                    ctccagaaaCAGTCCGCACCGCTTGGATTGGCCGTTCATAGCATTACCTCCATATAGATGTGTTGGAAACAATGTAGTAGACGCTTGCGTCTGCAACCTCTTGTGTGGCGCCTCACAGGCTTTCTACAAGTGCTggg                                                      <  8:318085/131‑1 (MQ=255)
                                                                                                    ctccacaAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTCCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTggg                                                      <  8:318087/134‑1 (MQ=255)
                                                                                                    cTGCACAAACATGCCGGCACGTTTGGATTTGGCGTTCATAGAATTTCCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCGTCACAGGCTTTCTAGAAGTGGTGGGtagattcgg                                             >  7:318085/1‑139 (MQ=255)
                                                                                                    cTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTCCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTggg                                                      >  7:318087/1‑137 (MQ=255)
                                                                                                       cacaAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTgg                                                       >  5:273875/1‑133 (MQ=255)
                                                                                                       cacaAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTgg                                                       <  6:273875/133‑1 (MQ=255)
                                                                                                                   gccACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCg                           <  5:345650/149‑1 (MQ=255)
                                                                                                                    ccACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCgg                          >  8:282847/1‑149 (MQ=255)
                                                                                                                      aCGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGc                        <  5:67540/149‑1 (MQ=255)
                                                                                                                        gTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGt                      >  8:161006/1‑149 (MQ=255)
                                                                                                                         tttGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTc                     <  4:203939/149‑1 (MQ=255)
                                                                                                                              aTTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTaaa                <  5:117406/149‑1 (MQ=255)
                                                                                                                               tttGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTaaa                <  7:161006/148‑1 (MQ=255)
                                                                                                                                     gTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTAAAGCGCACt         >  2:102483/1‑149 (MQ=255)
                                                                                                                                         aTAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTAAAGCGCACTGCa      <  5:193763/148‑1 (MQ=255)
                                                                                                                                          tAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTAAAGCGCACTGCAg     >  2:290376/1‑148 (MQ=255)
                                                                                                                                            gAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTAAAGCGCACTGCAGGAg  <  7:282847/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CTTGGGATAGTGTTAGGGCGGTGTAGCGGCATAGTGGCGAACAGAACGTGTAGCGGCGATTCGCTACAAACTCTTTCAAGCAGTGTTGGCAAGCGTTGACCTGCACAAACATGCCGCCACGTTTGGATTTGGCGTTCATAGAATTTTCTCCATATTGATGTGTTGGAAAGAATGTATTAGACGATAGCGTCTGCAAGCTCTTGTGAGGCGCCTCACAGGCTTTCTAGAAGTGCTGGGTATAGTAGGGTATGCCGCCTGCCAGCGGGCGTCTTAAAGCGCACTGCAGGAG  >  NC_002947/1280017‑1280305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑