Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,294,115 A→T 9.1% intergenic (+16/+24) pdxH → / ← PP_1130 pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,294,1150AT 9.1% 154.7 / 5.4 55intergenic (+16/+24)pdxH/PP_1130pyridoxine/pyridoxamine 5'‑phosphate oxidase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base T (3/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

AGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAG  >  NC_002947/1293969‑1294251
                                                                                                                                                  |                                                                                                                                        
aGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAg                                                                                                                                        >  5:4481/1‑149 (MQ=255)
         ggggATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCTTGGCGGCCCCAg                                                                                                                               <  4:349783/149‑1 (MQ=255)
            gATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCa                                                                                                                            >  7:38596/1‑149 (MQ=255)
                     tgttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCaa                                                                                                                    <  1:282959/148‑1 (MQ=255)
                       ttgCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGc                                                                                                                  <  5:86760/148‑1 (MQ=255)
                          cccGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                          >  5:262309/1‑137 (MQ=255)
                          cccGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCAtt                                                                                                                          <  6:262309/137‑1 (MQ=255)
                           ccGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGAC‑GGCAGTGGGGGCGGGGGCGGCGGGCGCCCGGGGCAACTGGGGCCGCCAAGCGGCCCCCGCCATTGGTGAAggggtggg                                                                                                            >  8:54876/1‑142 (MQ=255)
                             gAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGACATTGATCAAGCTACCCCg                                                                                                           >  3:174721/1‑149 (MQ=255)
                                  cATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATaa                                                                                                      <  8:130073/149‑1 (MQ=255)
                                         ttCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTgccg                                                                                               >  1:195320/1‑149 (MQ=255)
                                              gCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTcg                                                                                          >  6:258334/1‑149 (MQ=255)
                                                   ggcgggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTc                                                                                     <  8:38596/149‑1 (MQ=255)
                                                    catggcAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTcc                                                                                    <  6:65596/146‑1 (MQ=255)
                                                                   gCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGt                                                                     >  8:123183/1‑149 (MQ=255)
                                                                     aTGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGAt                                                                                                                       >  6:301427/1‑97 (MQ=255)
                                                                     aTGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGAt                                                                                                                       <  5:301427/97‑1 (MQ=255)
                                                                      tGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGc                                                                  <  1:176633/149‑1 (MQ=255)
                                                                            tttGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGc                                                            <  6:4481/149‑1 (MQ=255)
                                                                                 aCTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCAc                                                       <  5:213147/149‑1 (MQ=255)
                                                                                   tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGccc                                                          <  2:53596/144‑1 (MQ=255)
                                                                                   tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGccc                                                          >  1:53596/1‑144 (MQ=255)
                                                                                   tATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCt                                                     >  3:41686/1‑149 (MQ=255)
                                                                                        gCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTcc                                                <  4:41686/149‑1 (MQ=255)
                                                                                         cTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCt                                               >  1:320503/1‑149 (MQ=255)
                                                                                          tggtggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTg                                              >  5:68774/1‑149 (MQ=255)
                                                                                            gtggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGcc                                            <  2:195320/149‑1 (MQ=255)
                                                                                             tggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCcg                                           >  4:213783/1‑149 (MQ=255)
                                                                                              ggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAGGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTAAGCCCCACCTTCTCCTGCcgc                                          >  4:179915/1‑149 (MQ=255)
                                                                                              ggACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGTTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCcgc                                          >  2:45822/1‑149 (MQ=255)
                                                                                                aCGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGCATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCgcgc                                        <  5:258334/149‑1 (MQ=255)
                                                                                                    gCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAg                                                                                                                   <  2:351792/70‑1 (MQ=255)
                                                                                                    gCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAg                                                                                                                   >  1:351792/1‑70 (MQ=255)
                                                                                                    gCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTgc                                    >  6:282241/1‑149 (MQ=255)
                                                                                                          gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTgc                                                                                                 >  4:338683/1‑82 (MQ=255)
                                                                                                          gCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTgc                                                                                                 <  3:338683/82‑1 (MQ=255)
                                                                                                               gagaGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTc                         <  2:320503/149‑1 (MQ=255)
                                                                                                               gagaGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTc                         >  1:155993/1‑149 (MQ=255)
                                                                                                                 gagCGGCTGGCGCCCTGAGCAACTGGGGCGGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCa                       <  3:100781/149‑1 (MQ=255)
                                                                                                                    cGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTtctc                                                 >  1:207173/1‑120 (MQ=255)
                                                                                                                    cGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTtctc                                                 <  2:207173/120‑1 (MQ=255)
                                                                                                                     ggCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                   <  6:68774/149‑1 (MQ=255)
                                                                                                                     ggCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                   >  3:200929/1‑149 (MQ=255)
                                                                                                                      gCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCCGCAt                   >  2:291458/1‑148 (MQ=255)
                                                                                                                           cgcCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGTTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgc             <  1:45822/149‑1 (MQ=255)
                                                                                                                             cccTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAAcgcgc           <  7:123183/149‑1 (MQ=255)
                                                                                                                                     ggcTGGGGCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGttttt     >  6:21976/3‑147 (MQ=255)
                                                                                                                                     aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTc                         >  7:45748/1‑127 (MQ=255)
                                                                                                                                     aaCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTc                         <  8:45748/127‑1 (MQ=255)
                                                                                                                                      gcTGGGGCCGCTTGGCGGCCCCAGTCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTc    >  7:337379/2‑147 (MQ=255)
                                                                                                                                      gcTGGGGCCGCTTGGCGGCCCCAGTCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTc    <  8:337379/146‑1 (MQ=255)
                                                                                                                                      aCTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg  >  8:180408/1‑149 (MQ=255)
                                                                                                                                       cTGGGGCCGCTTGGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAg  >  3:333535/1‑148 (MQ=255)
                                                                                                                                       cTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                   <  7:215384/131‑1 (MQ=255)
                                                                                                                                       cTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCAt                   >  8:215384/1‑131 (MQ=255)
                                                                                                                                                  |                                                                                                                                        
AGCATTGGGGGGGATACCGTTTGTTGCCCGAGCGCATCGAGTTCTGGCAGGGGCGGGCAAGTCGTCTGCATGATCGTTTGAACTATCGGCTGGTGGACGGGCAGTGGCTGCGAGAGCGGCTGGCGCCCTGAGCAACTGGGGCCGCCAAGCGGCCCCAGCCATTGATCAAGCTACCCCGGATAACCTGCCGCCTCGCGCTCCAACCACCCCGCCAGTTGCTTACGCCCCACCTTCTCCTGCCGCGCCTGCGCCAACCTTTCCAGCATGAACGCGCGTTTTTCAG  >  NC_002947/1293969‑1294251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑