Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,366,656 A→G 7.5% L15P (CTC→CCC) ‡ PP_1191 ← S4 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,366,6560AG 7.5% 147.1 / 2.1 53L15P (CTC→CCC) ‡PP_1191S4 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (25/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGCTCCAGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCATTTTGGACACATAGCGCGG  >  NC_002947/1366509‑1366801
                                                                                                                                                    |                                                                                                                                                  
aCCGGCTCCAGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGa                                                                                                                                                    <  1:22418/148‑1 (MQ=255)
aCCGGCTCCAGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAg                                                                                                                                                   <  3:221790/149‑1 (MQ=255)
       ccAGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTc                                                                                                                                             >  1:148708/1‑148 (MQ=255)
         aGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACTCGCTCGGCCTCGCGGCGGGAGCAGCCGAgctgc                                                                                                                                               <  1:223623/145‑1 (MQ=255)
         aGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAgctgc                                                                                                                                               >  2:223623/1‑144 (MQ=255)
         aGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGAt                                                                                                                                          >  1:73139/1‑149 (MQ=255)
             tttCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAgg                                                                                                                                      <  7:130948/149‑1 (MQ=255)
                cGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGt                                                                                                                                  >  5:5589/1‑149 (MQ=255)
                       ggCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACa                                                                                                                           <  2:73139/149‑1 (MQ=255)
                              ggCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCgggg                                                                                                                    >  8:36807/1‑147 (MQ=255)
                                   cagcTCGAGGCTCTGCTTCTCAAGGTTTAACTGCGGCTGCTCGACCACCACGCCTTCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGTGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGtt                                                                                                               <  7:127384/149‑1 (MQ=255)
                                                 gctgctCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGCCCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGGTCGGACATGGGGCaa                                                                                                 >  1:90963/1‑149 (MQ=255)
                                                     ctCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAAtt                                                                                               <  1:12405/147‑1 (MQ=255)
                                                     ctCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCg                                                                                             <  4:246939/149‑1 (MQ=255)
                                                        aaCCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGc                                                                                            >  5:256400/1‑147 (MQ=255)
                                                          ccTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTAAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAgg                                                                                        >  3:292040/1‑149 (MQ=255)
                                                           cTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCTCGGCTGCTcccgc                                                                                                                                         <  8:62638/100‑5 (MQ=255)
                                                           cTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCTCGGCTGCTcccgc                                                                                                                                         >  7:62638/1‑96 (MQ=255)
                                                           cTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAgg                                                                                        >  2:107309/1‑148 (MQ=255)
                                                              gAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAg                                                                                     <  2:90963/148‑1 (MQ=255)
                                                               aaCTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCACTGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTGCGGACATGGGGCAATTCGCAAGGCAgg                                                                                    <  2:113541/148‑1 (MQ=255)
                                                                    cGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAg                                                                               <  6:5589/148‑1 (MQ=255)
                                                                           tCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCAt                                                                       >  7:335467/1‑149 (MQ=255)
                                                                           tCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCAt                                                                       >  6:152031/1‑149 (MQ=255)
                                                                             gACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGCGGCCTTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCAtt                                                                      <  1:107309/149‑1 (MQ=255)
                                                                             gACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGa                                                                                                           <  2:123982/111‑1 (MQ=255)
                                                                             gACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGa                                                                                                           >  1:123982/1‑111 (MQ=255)
                                                                               ccaccaCGCCATCGACCCTTACCCAGCCTCCTTGAATGTACT‑GCTCGTCCTCGCGTCGGGAGCAGCCTAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTa                                                                   <  7:222150/149‑1 (MQ=255)
                                                                                   cacGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTAcctg                                                               >  6:134510/1‑149 (MQ=255)
                                                                                         aTCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATg                                                                                                       >  5:89680/1‑103 (MQ=255)
                                                                                         aTCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATg                                                                                                       <  6:89680/103‑1 (MQ=255)
                                                                                          tCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCaa                                                        >  7:286230/1‑149 (MQ=255)
                                                                                              ccGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCgg                                                    <  8:286230/149‑1 (MQ=255)
                                                                                               cGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCgg                                                    >  6:19074/1‑148 (MQ=255)
                                                                                                     ccAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAgatga                                             >  7:245427/1‑149 (MQ=255)
                                                                                                        gCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATgg                                          <  2:148708/149‑1 (MQ=255)
                                                                                                           tcctTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACctgcctgc                                                          >  4:295067/1‑130 (MQ=255)
                                                                                                           tcctTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACctgcctgc                                                          <  3:295067/130‑1 (MQ=255)
                                                                                                            cctTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAAt                                      >  7:274701/1‑149 (MQ=255)
                                                                                                              ttCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCt                                    >  7:59652/1‑149 (MQ=255)
                                                                                                               tCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGa                                                                                                           >  4:97513/1‑77 (MQ=255)
                                                                                                               tCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGa                                                                                                           <  3:97513/77‑1 (MQ=255)
                                                                                                               tCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCtg                                   >  5:364089/1‑149 (MQ=255)
                                                                                                                     tACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCATGGAAAGGCCTCCATTGTAGCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCa                              <  6:43119/148‑1 (MQ=255)
                                                                                                                        t‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTgtgt                                >  7:256417/1‑143 (MQ=255)
                                                                                                                        t‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTgtgt                                <  8:256417/143‑1 (MQ=255)
                                                                                                                                 cTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCAttt                  <  5:19074/149‑1 (MQ=255)
                                                                                                                                 cTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCAttt                  >  8:63244/1‑149 (MQ=255)
                                                                                                                                         gggAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCATTTTGGAcaca          <  8:59652/149‑1 (MQ=255)
                                                                                                                                           gAGCAGCTCGGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGt                                                                                                                <  2:350054/45‑1 (MQ=255)
                                                                                                                                           gAGCAGCTCGGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGt                                                                                                                >  1:350054/1‑45 (MQ=255)
                                                                                                                                              cagcCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGGTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCATTTTGGACACATAgcg     <  8:335467/149‑1 (MQ=255)
                                                                                                                                                 ccGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCATTTTGGACACATAGCGCgg  <  6:256400/149‑1 (MQ=255)
                                                                                                                                                 ccGAGCTGCTCGATGAGG‑CGTTTGGAAAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCATTTTGGACACATAGCGAgg  >  1:61511/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACCGGCTCCAGGGTTTCGGCACGGGCGCCCGGCAGCAGCTCGACGCGCTGCTGCTCAACCTTGAACTGCGGCTGCTCGACCACCACGCCATCGACCGTTACCCAGCCTCCTTCAATGTACT‑GCTCGGCCTCGCGGCGGGAGCAGCCGAGCTGCTCGATGAGG‑CGTTTGGACAGGCGGACGGGTTCGGACATGGGGCAATTCGCAAGGCAGGGAAAGGCCTCCATTGTACCTGCCTGCAAGCGGTAGATGATGGGAATCTGTGTCAGATGCGCCATTTTGGACACATAGCGCGG  >  NC_002947/1366509‑1366801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑