Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,376,322 G→C 13.0% intergenic (+31/+18) kup → / ← PP_1201 potassium transport system protein Kup/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,376,3220GC13.0% 143.7 / 12.7 54intergenic (+31/+18)kup/PP_1201potassium transport system protein Kup/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base C (2/5);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

CGCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTG  >  NC_002947/1376178‑1376467
                                                                                                                                                |                                                                                                                                                 
cgcTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCg                                                                                                                                               <  3:349728/149‑1 (MQ=255)
     gCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCt                                                                                                                                          <  8:41416/149‑1 (MQ=255)
     gCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCGAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCt                                                                                                                                          >  8:262586/1‑149 (MQ=255)
     gCGGGGCAACCTGGTGCCGTTCTTTCTTAATCATTGCAACCGCAAATTTCGGTTCCTGAACCTTCCGTTGAACCGTGGCTTCGTGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCt                                                                                                                                          <  6:189262/149‑1 (MQ=255)
       ggggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTGa                                                                                                                                        <  2:225043/149‑1 (MQ=255)
       ggggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                        <  1:93876/149‑1 (MQ=255)
        gggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCCACCTGCCGGTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTGAg                                                                                                                                       <  6:111620/149‑1 (MQ=255)
        gggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAg                                                                                                                                       >  2:61696/1‑149 (MQ=255)
                     gcgTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTc                                                                                                                                >  6:73560/1‑143 (MQ=255)
                     gcgTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTc                                                                                                                                <  5:73560/143‑1 (MQ=255)
                                  agaaTGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGc                                                                                                              <  4:16439/148‑1 (MQ=255)
                                   gaaTGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTg                                                                                                            >  5:352311/1‑149 (MQ=255)
                                        ccAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACtt                                                                                                       <  4:81912/149‑1 (MQ=255)
                                         cAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTg                                                                                                      >  7:282317/1‑149 (MQ=255)
                                          aaCGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCa                                                                                                                     >  6:150175/1‑133 (MQ=255)
                                          aaCGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCa                                                                                                                     <  5:150175/133‑1 (MQ=255)
                                                    tGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGt                                                                                           >  3:87217/1‑149 (MQ=255)
                                                     gcgcTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGCCTTGCCGTGGCGCGtt                                                                                          >  7:176714/1‑149 (MQ=255)
                                                       gcTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTc                                                                                        <  6:352311/149‑1 (MQ=255)
                                                              cAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGATAAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTTCTCAGCTACGCTGGACTTGACTTGGAGCGTTTCGAtctag                                                                                 >  1:288432/1‑147 (MQ=255)
                                                                  cTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCa                                                                              >  8:44057/1‑148 (MQ=255)
                                                                       gTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAAc                                                                        >  5:216964/1‑149 (MQ=255)
                                                                       gTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAAc                                                                        <  1:61696/149‑1 (MQ=255)
                                                                       gTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAAc                                                                        <  6:56423/149‑1 (MQ=255)
                                                                               gggTGATCTAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGc                                                                <  4:87217/149‑1 (MQ=255)
                                                                                ggTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTc                                                                                        <  4:135810/124‑1 (MQ=255)
                                                                                ggTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTc                                                                                        >  3:135810/1‑124 (MQ=255)
                                                                                 gTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGa                                                              <  3:40428/149‑1 (MQ=255)
                                                                                   gATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGc                                                                                                              >  5:324560/1‑99 (MQ=255)
                                                                                   gATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGc                                                                                                              <  6:324560/99‑1 (MQ=255)
                                                                                      cGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                  >  8:218979/1‑124 (MQ=255)
                                                                                      cGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                  <  7:218979/124‑1 (MQ=255)
                                                                                        aGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCgg                                                       <  7:44057/149‑1 (MQ=255)
                                                                                                aCCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTtct                                               >  5:324929/1‑149 (MQ=255)
                                                                                                   cAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCtcg                                             >  3:267990/1‑148 (MQ=255)
                                                                                                        cGAGATCTGAATGGGGGTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAg                                       <  7:262586/149‑1 (MQ=255)
                                                                                                          agaTCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAgtg                                     <  6:324929/149‑1 (MQ=255)
                                                                                                                 aaTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGt                                                     >  8:252575/1‑126 (MQ=255)
                                                                                                                 aaTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGt                                                     <  7:252575/126‑1 (MQ=255)
                                                                                                                   tGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGc                             >  2:24731/1‑148 (MQ=255)
                                                                                                                      ggTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAgg                         >  7:309413/1‑149 (MQ=255)
                                                                                                                        ttATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCa                       <  8:309413/149‑1 (MQ=255)
                                                                                                                          ataCAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAg                      <  8:282317/148‑1 (MQ=255)
                                                                                                                            aCAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGACTTGGCGCGTTTGGATATCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTGTCGTCGAAGTGGTGCCCGCCAGGCAGtt                    >  5:6596/1‑148 (MQ=255)
                                                                                                                                cagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAgtggt                                   <  8:200929/129‑1 (MQ=255)
                                                                                                                                cagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAgtggt                                   >  7:200929/1‑129 (MQ=255)
                                                                                                                                aagGCTCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCggg                                                      >  2:26366/2‑110 (MQ=255)
                                                                                                                                aagGCTCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCggg                                                      <  1:26366/109‑1 (MQ=255)
                                                                                                                                   gCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATATCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGGTTCAGgcg             >  8:142333/1‑148 (MQ=255)
                                                                                                                                     tCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCt           >  6:102835/1‑148 (MQ=255)
                                                                                                                                     tCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCt           >  7:253849/1‑148 (MQ=255)
                                                                                                                                       aGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCAc        >  2:181951/1‑149 (MQ=255)
                                                                                                                                           ccGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTAc    >  1:58964/1‑149 (MQ=255)
                                                                                                                                             gCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTg  >  6:148909/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CGCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTG  >  NC_002947/1376178‑1376467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑