Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,383,254 A→T 9.5% L16Q (CTG→CAG)  PP_1204 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,383,2540AT 9.5% 163.6 / 7.2 63L16Q (CTG→CAG) PP_1204lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (29/27);  new base T (2/4);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

GTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCT  >  NC_002947/1383111‑1383394
                                                                                                                                               |                                                                                                                                            
gTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTgcg                                                                                                                                         >  8:5665/1‑149 (MQ=255)
  cGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTgcggc                                                                                                                                       <  7:70034/149‑1 (MQ=255)
     tGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGcc                                                                                                                                    >  7:80404/1‑149 (MQ=255)
       cGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTGCGGCGccc                                                                                                                                   <  2:8283/148‑1 (MQ=255)
       cGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTGCGGCGcc                                                                                                                                    <  6:164546/147‑1 (MQ=255)
         gAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCACTGACGAGCAGGGCGCCGCACTGTGCGGCGccct                                                                                                                                  <  8:216891/147‑2 (MQ=255)
         gAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCACTGACGAGCAGGGCGCCGCACTGTGCGGCGccct                                                                                                                                  >  7:216891/1‑146 (MQ=255)
          aaGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCaa                                                                                                                               >  1:127888/1‑149 (MQ=255)
                cATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTa                                                                                                                          >  4:115536/1‑148 (MQ=255)
                 aTCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTaaa                                                                                                                        <  7:5665/149‑1 (MQ=255)
                    aCCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAgg                                                                                                                      >  5:349777/1‑148 (MQ=255)
                         cGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACtt                                                                                                                 <  3:30210/148‑1 (MQ=255)
                         cGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACtt                                                                                                                 <  5:90611/148‑1 (MQ=255)
                          ggCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCCCAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCg                                                                                                               >  7:159743/1‑149 (MQ=255)
                                 cGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTGCGGCGcc                                                                                                                                    <  7:186526/121‑1 (MQ=255)
                                 cGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACTGTGCGGCGcc                                                                                                                                    >  8:186526/1‑121 (MQ=255)
                                     aTCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGa                                                                                                    >  6:121842/1‑149 (MQ=255)
                                     aTCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGa                                                                                                    >  3:113992/1‑149 (MQ=255)
                                       cGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTaaa                                                                                                                        <  1:134110/127‑1 (MQ=255)
                                       cGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTaaa                                                                                                                        >  2:134110/1‑127 (MQ=255)
                                        gATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATc                                                                                                  <  2:127888/148‑1 (MQ=255)
                                         aTCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCt                                                                                                             <  3:250641/136‑1 (MQ=255)
                                         aTCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCt                                                                                                             >  4:250641/1‑136 (MQ=255)
                                         aTCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTGGGCGCAGCCGCGGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCAc                                                                                                <  2:258077/149‑1 (MQ=255)
                                          tCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAACGGGAAAGGTTCTTGGCTTGTTCATGGTCACt                                                                                               >  4:145000/1‑149 (MQ=255)
                                            tGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACGTCGCTGGGACATGATGACTCg                                                                                             >  8:268123/1‑149 (MQ=255)
                                             gCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTAACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGt                                                                                            >  5:349159/1‑149 (MQ=255)
                                                       cTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGTCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGtt                                                                                   <  3:115536/148‑1 (MQ=255)
                                                              aGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAgcgg                                                                           <  6:368370/149‑1 (MQ=255)
                                                               gTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAgcggc                                                                          <  4:113992/149‑1 (MQ=255)
                                                               gTTTGCGCACGACGCGCACCTCATGGGCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAgcggc                                                                          <  2:75260/149‑1 (MQ=255)
                                                                     gcTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGaa                                                                       >  5:276628/1‑146 (MQ=255)
                                                                     gcTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGaa                                                                       <  6:276628/146‑1 (MQ=255)
                                                                       tCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                  <  5:121842/149‑1 (MQ=255)
                                                                       tCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGGAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                  >  3:59709/1‑149 (MQ=255)
                                                                         gACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCGTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                  <  7:268123/147‑1 (MQ=255)
                                                                                tcctcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAg                                                          >  6:136496/1‑148 (MQ=255)
                                                                                   tcATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAg                                                      >  1:296219/1‑149 (MQ=255)
                                                                                     aTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGc                                                            <  1:274047/141‑1 (MQ=255)
                                                                                     aTGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGc                                                            >  2:274047/1‑141 (MQ=255)
                                                                                      tGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTc                                                   <  2:1187/149‑1 (MQ=255)
                                                                                       ggTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTAc                                                                                                        >  8:236494/1‑95 (MQ=255)
                                                                                       ggTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTAc                                                                                                        <  7:236494/95‑1 (MQ=255)
                                                                                        gTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACtt                                                                                                                 >  7:76006/1‑85 (MQ=255)
                                                                                        gTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACtt                                                                                                                 <  8:76006/85‑1 (MQ=255)
                                                                                         tCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                  >  3:203469/1‑131 (MQ=255)
                                                                                         tCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCgg                                                                  <  4:203469/131‑1 (MQ=255)
                                                                                         tCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGc                                                            <  6:70680/137‑1 (MQ=255)
                                                                                         tCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGc                                                            >  5:70680/1‑137 (MQ=255)
                                                                                           gctgcgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCAc                                              <  5:359426/149‑1 (MQ=255)
                                                                                           gctgcgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCCCACAGTGCGGCGCCCCCAAGGGTAAAAGTACTTCGCTTGTACATGATCACTCGTGCATTTGGTATAAGCGGCGAACGCGCGCCTGCAGGTAGGTAAGCAc                                              >  4:185665/1‑149 (MQ=255)
                                                                                               cgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACtt                                            <  6:349777/147‑1 (MQ=255)
                                                                                               cgctgGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCa                                          <  5:136496/149‑1 (MQ=255)
                                                                                                 cggggACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACCGGGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCAtt                                        <  8:80404/147‑1 (MQ=255)
                                                                                                     gACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGc                                    <  2:296219/149‑1 (MQ=255)
                                                                                                          cTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTAGCTTGTACATTAACAATAGTGCATTGGTTATCAGCGGCGAACGCGGGCCAGCACGAAggg                                                    >  2:256567/1‑127 (MQ=255)
                                                                                                           tGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAAc                              >  1:241556/1‑149 (MQ=255)
                                                                                                             gTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGc                            >  8:28763/1‑149 (MQ=255)
                                                                                                                 gcgcAGCCGCTTACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTgg                        <  6:349159/149‑1 (MQ=255)
                                                                                                                   gcAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGc                      <  7:28763/149‑1 (MQ=255)
                                                                                                                                   aGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTcggtgcgg      >  5:143175/1‑149 (MQ=255)
                                                                                                                                    gggCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTcggtgcggt     >  6:137078/1‑149 (MQ=255)
                                                                                                                                       cgccgcACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCt  >  8:300033/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                            
GTCGCTGCGGAAGCTCCATCACCTTCGGCTCGCCGACATCGATCTGCAGGGTATGCTCGAGCAGTTTGCGCTCGACGCGCTCCTCATGGTCGCTGCGCTGGGACATCTGGTTGGCGCAGCCGCTGACGAGCAGGGCGCCGCACAGTGCGGCGCCCCCAAGGGTAAAGGTACTTCGCTTGTACATGATCACTCGTGCATTGGTTATCAGCGGCGAACGCGGGCCTGCAGGAAGGTCAGCACTTCATTGAGCGGCAACGCCTGGGCGTCCTGCTCGGTGCGGTGCT  >  NC_002947/1383111‑1383394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑