Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,384,505	G→T	7.8%	R176S (CGC→AGC)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,384,505	0	G	T	7.8%	198.5 / 4.7	64	R176S (CGC→AGC)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (32/27);  new base T (3/2);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CGCTGAAGATCACGTCATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGT  >  NC_002947/1384358‑1384653                                                                                                                                                    |                                                                                                                                                     tggtGAAGATGACGTGATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGa                                                                                                                                                     >  5:121419/4‑149 (MQ=255) cGCTGAAGATCTCGTCATCTTCAGCAGACTCTGCCAGGACGTGGAACTCGTGCGAGTCGCTGCCCCCGAGCGAGACGGTGGCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACTCATTGCTGTACGCCTGGTGCATGCGa                                                                                                                                                     <  2:116404/149‑1 (MQ=255)  gCTGAAGATCACGTCATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGAt                                                                                                                                                    <  7:130256/149‑1 (MQ=255)      aaGATCACGTCATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCa                                                                                                                                                  >  6:156071/1‑147 (MQ=255)          tCACGTCATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGtt                                                                                                                                            >  3:2821/1‑149 (MQ=255)             cGTCATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTcc                                                                                                                                         >  4:67722/1‑149 (MQ=255)             cGTCATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTcc                                                                                                                                         <  6:234033/149‑1 (MQ=255)                 aTCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTg                                                                                                                                       >  1:51983/1‑147 (MQ=255)                    ttCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAgg                                                                                                                                   >  3:314838/1‑148 (MQ=255)                       aCCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGaa                                                                                                                                <  5:156071/148‑1 (MQ=255)                       aCCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAg                                                                                                                               >  3:154709/1‑149 (MQ=255)                          aGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCTTAGGTTTCCTGCAGGGAAGCCt                                                                                                                            >  8:60425/1‑149 (MQ=255)                           gACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTg                                                                                                                           >  2:341540/1‑149 (MQ=255)                                  ccAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCgg                                                                                                                      <  4:154709/147‑1 (MQ=255)                                  ccAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCa                                                                                                                    >  7:339679/1‑149 (MQ=255)                                  ccAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCa                                                                                                                    >  6:293689/1‑149 (MQ=255)                                    aGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATg                                                                                                                  >  4:121268/1‑149 (MQ=255)                                      cACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGa                                                                                                                <  4:2821/149‑1 (MQ=255)                                        cGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAAc                                                                                                              <  8:339679/149‑1 (MQ=255)                                          tGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAAc                                                                                                              >  8:312739/1‑147 (MQ=255)                                             aaCTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTa                                                                                                         <  1:341540/149‑1 (MQ=255)                                                    gCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTcc                                                                                                  >  8:8260/1‑149 (MQ=255)                                                        gTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGt                                                                                                                         >  4:158151/1‑122 (MQ=255)                                                           gCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCatga                                                                                           <  7:8260/149‑1 (MQ=255)                                                               cccccGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGt                                                                                                          <  2:46460/130‑1 (MQ=255)                                                               cccccGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGt                                                                                                          >  1:46460/1‑130 (MQ=255)                                                                ccccGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAGGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAAc                                                                                      <  2:51983/149‑1 (MQ=255)                                                                 cccGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTc                                                                                                                        <  3:6845/114‑1 (MQ=255)                                                                 cccGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTc                                                                                                                        >  4:6845/1‑114 (MQ=255)                                                                 cccGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACt                                                                                     >  5:111609/1‑149 (MQ=255)                                                                  ccGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTc                                                                                    >  7:26706/1‑149 (MQ=255)                                                                    gATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCTATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTcgc                                                                                  >  4:162802/1‑149 (MQ=255)                                                                    gATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCTATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTcgc                                                                                  >  4:168372/1‑149 (MQ=255)                                                                    gATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCTTAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTcgc                                                                                  <  7:60425/149‑1 (MQ=255)                                                                    gATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTcgc                                                                                  <  3:67722/149‑1 (MQ=255)                                                                                gtCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCa                                                                      <  8:26706/149‑1 (MQ=255)                                                                                   ggCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGc                                                                   <  5:293689/149‑1 (MQ=255)                                                                                    gCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAGGCCTGGTCgg                                                                                                                      >  6:60062/1‑97 (MQ=255)                                                                                    gCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAGGCCTGGTCgg                                                                                                                      <  5:60062/97‑1 (MQ=255)                                                                                         cACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCATGGACGGCATGGAACGAGTAGGCGTCCTACATGATGAAAACGCGCCCACGCATCCGGCCGaacc                                                             >  4:139766/1‑147 (MQ=255)                                                                                            tGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGCTGCATGCGATCAAAGGTTTCCTGCAGGGAAGCCTGGTCGGAATGGAAAGAGTAGGCGTCATTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGcgcg                                                          >  5:159686/1‑149 (MQ=255)                                                                                               gCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCgcgggc                                                       >  1:340961/1‑149 (MQ=255)                                                                                                        caggcccaggcGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGAtc                                                  <  5:142296/145‑1 (MQ=255)                                                                                                        caggcccaggcGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGAtc                                                  >  6:142296/1‑145 (MQ=255)                                                                                                        caggcccaggcGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCATCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGt                                              >  1:116180/1‑149 (MQ=255)                                                                                                             ccaggcGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACg                                          >  5:123174/1‑148 (MQ=255)                                                                                                                    ggTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTgg                                  <  7:312739/149‑1 (MQ=255)                                                                                                                        aaCACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTg                              >  3:259932/1‑149 (MQ=255)                                                                                                                          cacaTTGCTGTACGTATTGTGAATGCTATAATAGGTTTCCTGCAGGGAAGCCTGGTCAGCATGTAAAGAGTAGGCTTCCTTCATGATGAACTAGCGGCCAAGCATCAGGCAGAATCTCGGGCGGATCTCGACACGGAACTTTGtattga                            >  8:318752/1‑144 (MQ=255)                                                                                                                             aTTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCt                         <  6:111609/149‑1 (MQ=255)                                                                                                                             aTTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCt                         >  4:55132/1‑149 (MQ=255)                                                                                                                             aTTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCt                         >  8:32491/1‑149 (MQ=255)                                                                                                                             aTTGCTCTACGCCTGGTGCATGCGATCATAGCATTCCTGCAGGGAAGCCTGGTCGGCATCCAACGAGTAGGCGACCTTCATCATGAACACGCGCCCACGCAACAGGCCGAAGCGCGGCCGGATCTCGTCACGGACCTAGGTCTGGATCt                         >  3:215380/1‑149 (MQ=255)                                                                                                                                gCTGTACGCCTGGTGCATGCTATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTgg                       <  3:168372/148‑1 (MQ=255)                                                                                                                                gCTGTACGCCTGGTGCATGCTATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTgg                       <  3:162802/148‑1 (MQ=255)                                                                                                                                 cTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTa                     <  3:121268/149‑1 (MQ=255)                                                                                                                                    tACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTAc                    <  6:123174/147‑1 (MQ=255)                                                                                                                                     aCGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAgg                 >  1:8907/1‑149 (MQ=255)                                                                                                                                            gTGGATTCGTTCATATGTTTCCTGGATGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAg            <  3:139766/147‑1 (MQ=255)                                                                                                                                            gTGCATGCGATCATAGTTTTCCTGCAGGGAAGGCTGGTCGGCTTGGAACGAGTAGGCGTCGTTCATGATGAACTCGCGGCCTCGCATCAGGCCGTAGCGCGGGCGGAGCTCGTCACGGATGTTGGTCTGGATCTGGTAGAGGTTGAGc           <  4:215380/148‑1 (MQ=255)                                                                                                                                            gTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAg            <  2:8907/147‑1 (MQ=255)                                                                                                                                            gTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAg            <  2:116180/147‑1 (MQ=255)                                                                                                                                            gTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGc           <  4:314838/148‑1 (MQ=255)                                                                                                                                              gCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCgg         >  7:218039/1‑148 (MQ=255)                                                                                                                                                    gATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGt  <  2:343017/149‑1 (MQ=255)                                                                                                                                                    gATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGt  >  2:138247/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CGCTGAAGATCACGTCATCTTCACCAGACTCTGCCAGCACGTGGAACTCGTGCGAGTAGCTGCCCCCGATCGAGCCGGTGTCGGCCTGCACTGGGCGGAAGTCCAGGCCCAGGCGGGTGAACACATTGCTGTACGCCTGGTGCATGCGATCATAGGTTTCCTGCAGGGAAGCCTGGTCGGCATGGAACGAGTAGGCGTCCTTCATGATGAACTCGCGGCCACGCATCAGGCCGAAGCGCGGGCGGATCTCGTCACGGAACTTGGTCTGGATCTGGTACAGGTTGAGCGGCAGCTGT  >  NC_002947/1384358‑1384653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑