| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,386,621 | T→G | 7.0% | intergenic (‑165/‑526) | oprD ← / → PP_1207 | basic amino acid specific porin OprD/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,386,621 | 0 | T | G | 7.0% | 176.9 / 5.0 | 57 | intergenic (‑165/‑526) | oprD/PP_1207 | basic amino acid specific porin OprD/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (24/29); new base G (2/2); total (26/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
GTTTTTAGGAAGAGTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTGGTAACCCATGTCGCTGTTGTTA > NC_002947/1386474‑1386759 | gTTTTTAGGAAGAGTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCtt > 7:285392/1‑149 (MQ=255) tttAGGAAGAGTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCtttt < 4:294678/148‑1 (MQ=255) ttAGGAAGAGTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTa < 2:191904/148‑1 (MQ=255) ggAAGAGTACAGCTGAGTCCAATTCATTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGt < 6:245085/148‑1 (MQ=255) gAAGAGTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATACGCACTATTTGCGGCACTTTTTCACCGGGTGGCTGTTAAGTAt > 8:307786/1‑149 (MQ=255) aaGAGTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTAtt < 5:30675/149‑1 (MQ=255) agagTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTTCGGAACTTTTTCACCGTGTCGCTTTTAAGTAttt > 5:359182/1‑149 (MQ=255) tACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCtttt > 4:311907/1‑137 (MQ=255) tACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCtttt < 3:311907/137‑1 (MQ=255) tGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATg < 6:355859/144‑1 (MQ=255) tGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATg > 5:355859/1‑144 (MQ=255) ccAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATc < 5:285506/148‑1 (MQ=255) aaGTCTTTTAGTTATATGGGGCAGCTCTTTGTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGATATAATGAATCTTTGAGGAAATTTTTAACAGTTTCTCTTTTAATTATTTAAATGTCGAAAATCAc > 5:274737/1‑148 (MQ=255) aaGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACg < 6:359182/149‑1 (MQ=255) gTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGcc > 7:20769/1‑149 (MQ=255) aGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGtt < 2:299321/148‑1 (MQ=255) atatGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAgg < 7:307786/148‑1 (MQ=255) ggggCAGATCTTTCTTTTTATGTAGGCGAAAATGTATAGCACGAAGACTGTTGTTGGCGATATGTCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTAGCAAATCACGCCCCGGTTCAGGGGCCg > 2:138125/1‑149 (MQ=255) ggCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGta < 3:150100/149‑1 (MQ=255) gCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAAc < 8:285392/149‑1 (MQ=255) gCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCGTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATaa > 4:224339/1‑148 (MQ=255) gCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACAGTGTCGCGTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAAc > 4:228838/1‑149 (MQ=255) tctttcttTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCAGTATAACCt > 6:336480/1‑149 (MQ=255) tttctttTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCt > 2:341774/1‑147 (MQ=255) aTGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTAtt < 8:20769/148‑1 (MQ=255) tGTCGGCGAAAATGTATAGCACGCAGACTGGTGTTGGCGATATGGCTATAAGCACTCTTTGCGTAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATCTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTc < 2:268695/148‑1 (MQ=255) ggCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCAAGGTTCAGGGTCAGTATAACATAAGCGCTATTCCTga > 7:186618/1‑147 (MQ=255) gAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTAtt > 2:31129/1‑140 (MQ=255) gAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTAtt < 1:31129/140‑1 (MQ=255) aaaTGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTAt < 3:13566/84‑1 (MQ=255) aaaTGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTAt > 4:13566/1‑84 (MQ=255) gTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGTCGTATAACCTCAGCGCTATTCCTGTc > 6:202940/1‑140 (MQ=255) gTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGTCGTATAACCTCAGCGCTATTCCTGTc < 5:202940/140‑1 (MQ=255) taGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCGTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAAt < 3:228838/149‑1 (MQ=255) taGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCGTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAAt < 3:224339/149‑1 (MQ=255) aGCACGCAGACTGTTGTTGCCGATATGGCTATAAGCACACTTTGCGGAACTTTTTAACCGTGTCGCTTTTAAGTATTTCAATGTCGCAAATCACGCCCAGGTTCAGGTGCCGTATAAAGTCAGCGCTATTCATCTAAAAGTAACGAATc > 5:279882/1‑149 (MQ=255) aCGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTg > 5:90794/1‑148 (MQ=255) tgttgtTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAActgct > 5:22113/1‑148 (MQ=255) tgttgtTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAActgct > 7:75337/1‑148 (MQ=255) ttgGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTcc > 1:96292/1‑149 (MQ=255) ttgGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTcc > 5:190720/1‑149 (MQ=255) tgGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCTCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTcc < 8:75337/148‑1 (MQ=255) ggCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGTGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCg < 1:138125/148‑1 (MQ=255) gCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCgg < 6:22113/148‑1 (MQ=255) tatGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAg < 6:90794/149‑1 (MQ=255) tatGGCGATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAAGCACGCCCCGGTTCAGGGGCCGGATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGa < 8:83872/148‑1 (MQ=255) atGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAAt < 6:361596/124‑1 (MQ=255) atGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAAt > 5:361596/1‑124 (MQ=255) atGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAg > 8:277439/1‑148 (MQ=255) gCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTg < 7:277439/149‑1 (MQ=255) gCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTg > 2:232043/1‑149 (MQ=255) gCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTg < 1:341774/149‑1 (MQ=255) cACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTgg < 6:190720/149‑1 (MQ=255) cACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTgg < 1:232043/149‑1 (MQ=255) tttCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAAc < 2:240958/125‑1 (MQ=255) tttCACAGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAAc > 1:240958/1‑125 (MQ=255) ttCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTGGTAACCCATGTCGCtgttg > 3:169923/1‑149 (MQ=255) tCACCGTGTCGCTTTTAAGTATTTTCTTGTAGCAAATCACGCACCGGTTCTGGGGCCGTATAACCTCAGCGCTAATCCTTTCTGCGTATCGAATCTCTAACTGCTGATTGCGGTTGTGGAAACTGCCTGGTAAACCATGTCGCttttg < 6:279882/148‑1 (MQ=255) aCCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTGGt < 4:175866/129‑1 (MQ=255) aCCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTGGt > 3:175866/1‑129 (MQ=255) aCCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTGGTAACCCATGTCGCTGTTGTTa < 2:96292/149‑1 (MQ=255) | GTTTTTAGGAAGAGTACCGCTGCGTCCAAGTCTTTTAGTTATATGGGGCAGCTCTTTCTTTTTATGTCGGCGAAAATGTATAGCACGCAGACTGTTGTTGGCGATATGGCTATAAGCACTCTTTGCGGAACTTTTTCACCGTGTCGCTTTTAAGTATTTCCATGTCGCAAATCACGCCCCGGTTCAGGGGCCGTATAACCTCAGCGCTATTCCTGTCACCGTAACGAATCTGTAACTGCTGATTCCGGTTGAGGAAACTCCCTGGTAACCCATGTCGCTGTTGTTA > NC_002947/1386474‑1386759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |