Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,418,249 G→T 7.4% L236L (CTG→CTT)  purC → phosphoribosylaminoimidazole‑succinocarboxamide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,418,2490GT 7.4% 181.1 / 3.9 62L236L (CTG→CTT) purCphosphoribosylaminoimidazole‑succinocarboxamide synthase
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base T (2/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

GGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTG  >  NC_002947/1418109‑1418389
                                                                                                                                            |                                                                                                                                             
ggCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCg                                                                                                                                         <  2:145563/147‑1 (MQ=255)
              cccGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAgc                                                                                                                         <  2:113347/149‑1 (MQ=255)
                cGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAgcgc                                                                                                                       >  7:254698/1‑149 (MQ=255)
                cGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAgcgc                                                                                                                       <  4:344973/149‑1 (MQ=255)
                 ggACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGcc                                                                                                                      >  6:139985/1‑149 (MQ=255)
                       cTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATAc                                                                                                                >  8:294873/1‑149 (MQ=255)
                        tGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATAc                                                                                                                >  7:209014/1‑148 (MQ=255)
                                     aCAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGAttttttt                                                                                                  >  8:249888/1‑149 (MQ=255)
                                       aaGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTg                                                                                                 >  4:138170/1‑148 (MQ=255)
                                       aaGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTg                                                                                                 >  2:259308/1‑148 (MQ=255)
                                         ggAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCGGTTAGCGGCACGTTAACGCAAGCGCCTGATAACACGCGATTTTTTTGaa                                                                                               >  5:48536/1‑148 (MQ=255)
                                               ccGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGa                                                                                       <  7:294873/149‑1 (MQ=255)
                                                 gCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCAGAAACCACGCGATTTTTTTGAAAAAA‑AGAtt                                                                                     >  2:138199/1‑149 (MQ=255)
                                                       agaTGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGtt                                                                               <  6:227292/149‑1 (MQ=255)
                                                        gaTGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAg                                                                                                                                           <  8:11473/89‑1 (MQ=255)
                                                         aTGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGttt                                                                              >  3:347424/1‑148 (MQ=255)
                                                             aCAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTaa                                                                           >  6:71312/1‑147 (MQ=255)
                                                              cAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTaa                                                                           >  4:347746/1‑146 (MQ=255)
                                                               aaGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCGGGAAGCGGCCCGTTCACCCCAGCGCCGGATACCCCGCGATTTTTTTTAAAAAA‑AGATTTGCGTTTTaaaat                                                                        >  1:351751/1‑147 (MQ=255)
                                                                    ccGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCt                                                                  <  4:347424/149‑1 (MQ=255)
                                                                            gCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTaaaa                                                                         <  2:154689/134‑1 (MQ=255)
                                                                            gCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTaaaa                                                                         >  1:154689/1‑134 (MQ=255)
                                                                            gCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTaaaa                                                                         >  3:150134/1‑134 (MQ=255)
                                                                            gCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTaaaa                                                                         <  4:150134/134‑1 (MQ=255)
                                                                                     tGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCAt                                                 >  8:236950/1‑149 (MQ=255)
                                                                                       ggCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTg                                               <  7:249888/149‑1 (MQ=255)
                                                                                          gACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTg                                               >  6:2206/1‑146 (MQ=255)
                                                                                           aCGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgaga                                           <  5:265709/149‑1 (MQ=255)
                                                                                            cGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAAGAGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgaga                                           <  1:259308/149‑1 (MQ=255)
                                                                                            cGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGgagag                                          <  7:130459/149‑1 (MQ=255)
                                                                                                   gAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTACCGCAAGCGCCTGGTACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTTTGTTGAGCGGCATTGGATCGATGCggg                                   >  4:202469/1‑149 (MQ=255)
                                                                                                    aaGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGa                                  <  7:236950/149‑1 (MQ=255)
                                                                                                       ccTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTg                               <  3:347746/149‑1 (MQ=255)
                                                                                                         tACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGt                             >  7:165273/1‑149 (MQ=255)
                                                                                                           cGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTc                            <  8:209014/148‑1 (MQ=255)
                                                                                                             aagaagTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGaa                         >  3:273802/1‑149 (MQ=255)
                                                                                                               gaagTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACg                       <  4:273802/149‑1 (MQ=255)
                                                                                                                 agTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACggg                     <  8:254698/149‑1 (MQ=255)
                                                                                                                 agTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACgg                      <  5:2206/148‑1 (MQ=255)
                                                                                                                  gTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGa                    >  1:196839/1‑149 (MQ=255)
                                                                                                                  gTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTAACGCAAGCGCCGGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGa                    >  6:308288/1‑149 (MQ=255)
                                                                                                                     gCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATgcgc                                                      <  7:219462/112‑1 (MQ=255)
                                                                                                                      ccAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTc                            <  2:220727/137‑1 (MQ=255)
                                                                                                                      ccAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTc                            >  1:220727/1‑137 (MQ=255)
                                                                                                                       cAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGAt               >  3:308168/1‑149 (MQ=255)
                                                                                                                        aaGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGAtt              >  8:21672/1‑149 (MQ=255)
                                                                                                                          gcgcCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTc             >  8:175585/1‑148 (MQ=255)
                                                                                                                              cTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTTAAAGAACGCTGGTATGATGCGCGGGATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGaaa         >  5:228319/1‑148 (MQ=255)
                                                                                                                              cTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAAt        >  3:107840/1‑149 (MQ=255)
                                                                                                                                 ggCGTGCCTCTTTAAGCGGCACGTTCACGCAAGCGCCTGATACCAGGCGATTTTTTTGAAAAAA‑ATATTTGCGTTTTAAAAGAACGCTGGTATAATGCGCGGCATTGGAGAGATGCCGGAGGGGTCGAACGGGACGGATTCGAAATCCg     <  1:138199/149‑1 (MQ=255)
                                                                                                                                 ggCGTGCCGCTGTACGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATc       <  2:351751/147‑1 (MQ=255)
                                                                                                                                 ggCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATcc      <  8:165273/148‑1 (MQ=255)
                                                                                                                                 ggCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATc       <  5:71312/147‑1 (MQ=255)
                                                                                                                                   cGTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGt    <  2:20964/148‑1 (MQ=255)
                                                                                                                                   cGTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGt    >  1:20964/1‑148 (MQ=255)
                                                                                                                                   cGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt   <  7:21672/149‑1 (MQ=255)
                                                                                                                                   cGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt   <  1:139534/149‑1 (MQ=255)
                                                                                                                                   cGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt   <  3:138170/149‑1 (MQ=255)
                                                                                                                                   cGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt   <  2:196839/149‑1 (MQ=255)
                                                                                                                                    gTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt   >  2:310709/1‑148 (MQ=255)
                                                                                                                                    gTGCCGCTTACAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGtt   <  1:310709/148‑1 (MQ=255)
                                                                                                                                     tGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTg  >  3:31355/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                             
GGCGACGAGTTCAGCCCGGACGGCTGCCGCCTGTGGGACAAGGAAACCCGCAAGAAGATGGACAAGGACCGCTTCCGCCAGGGCCTGGGCGACGTGATCGAAGCCTACGAAGAAGTTGCCAAGCGCCTGGGCGTGCCGCTGTAAGCGGCACGTTCACGCAAGCGCCTGATACCACGCGATTTTTTTGAAAAAA‑AGATTTGCGTTTTAAAAGAACGCTGGTATGATGCGCGGCATTGGAGAGATGCCGGAGTGGTCGAACGGGACGGATTCGAAATCCGTTG  >  NC_002947/1418109‑1418389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑