| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 85,066 | T→C | 13.6% | intergenic (+44/+86) | aroE → / ← PP_0075 | shikimate 5‑dehydrogenase/choline sulfate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 85,066 | 0 | T | C | 13.6% | 88.3 / 6.0 | 38 | intergenic (+44/+86) | aroE/PP_0075 | shikimate 5‑dehydrogenase/choline sulfate transporter |
| Reads supporting (aligned to +/- strand): ref base T (14/18); new base C (3/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
CTGGCTGAGCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGA > NC_002947/84921‑85210 | ctggctgAGCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTcc < 2:92858/149‑1 (MQ=255) gctgAGCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCcac < 7:329733/149‑1 (MQ=255) tgAGCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTccc > 4:63605/1‑145 (MQ=255) tgAGCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTccc < 3:63605/145‑1 (MQ=255) ggcagCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑cg < 1:233381/149‑1 (MQ=255) agCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTACCACAAAGTA‑cgtcg > 1:1714/1‑149 (MQ=255) aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGc < 7:210966/149‑1 (MQ=255) tcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACg < 2:288760/142‑1 (MQ=255) tcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGATGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACg > 1:288760/1‑142 (MQ=255) cttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCct > 5:346385/1‑149 (MQ=255) tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctc < 5:184516/147‑1 (MQ=255) tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctc < 4:160081/147‑1 (MQ=255) gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg < 7:282581/145‑1 (MQ=255) gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg < 5:214303/145‑1 (MQ=255) gcggggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg < 5:219522/145‑1 (MQ=255) gcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCCCTTTGCGGCCCAATCGCGACGCAAGGCCGTTACAACAAAGTA‑CCTCGTACGCCGCTCCTCATTGGGAgccgcc > 8:144556/1‑149 (MQ=255) gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCtt < 7:144556/149‑1 (MQ=255) gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCtt < 5:8884/149‑1 (MQ=21) ggCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGCCGCAAGGCCGTTCCCACACAGTA‑CCTCCTACGCCGGGGCTCAGTGGGAGCGGGCGTGGGGCGCGATGGGc > 2:189188/1‑149 (MQ=255) cgcCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTg < 6:342210/149‑1 (MQ=255) tgggcgcaaCGGCGCCGGCAACTCGCTCCGCGCTGAGCGGGCCGGGGCCGCTTGGCGGCCCGACCGCGACGCCAGGCCGTGCCCACAAAGTA‑CCTCGTACGCCGCTCCTCAGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCa < 5:184312/141‑1 (MQ=255) ccGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGATCCCCCAAAGTA‑CGTCGTACCCCGATCCTCAGTGGGAGCGGCCTTGCCTCGCGATGGGCTGCAAAGCAGAACCAGAAACCCaa > 6:103771/1‑149 (MQ=39) ctcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAAt > 5:157166/1‑137 (MQ=255) ctcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGGCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAAt < 6:157166/137‑1 (MQ=255) gCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc < 8:275379/143‑1 (MQ=255) gCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc > 7:275379/1‑143 (MQ=255) gatagggCCGCCAAGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGca > 5:330392/5‑149 (MQ=255) gCTTGGCGGCCATATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGCATCCCAACCCCATTCCGCAAACCGGATCGGGCACAACTcccc > 4:256172/1‑147 (MQ=255) cccaTCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc > 2:104283/4‑149 (MQ=17) ccTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc > 3:296073/1‑149 (MQ=255) ccaTCGCGACGCAAGGCCGCTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCa < 4:78503/144‑1 (MQ=255) ccaTCGCGACGCAAGGCCGCTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCa > 3:78503/3‑146 (MQ=255) gcgACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTg > 6:317638/1‑148 (MQ=255) cgACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc > 5:322090/1‑148 (MQ=255) aaGGCCGTTCCCACAATGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcct < 5:103771/149‑1 (MQ=255) ggCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATACCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCg < 2:239445/149‑1 (MQ=255) gCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcctc > 2:77986/1‑147 (MQ=255) gCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATAGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGa > 2:97975/1‑149 (MQ=21) | CTGGCTGAGCAGGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGA > NC_002947/84921‑85210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |