| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,428,977 | C→G | 8.6% | P360A (CCA→GCA) ‡ | mqo‑II → | malate:quinone oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,428,977 | 0 | C | G | 8.6% | 179.6 / 7.2 | 58 | P360A (CCA→GCA) ‡ | mqo‑II | malate:quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (27/26); new base G (3/2); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCC > NC_002947/1428836‑1429123 | gACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTg < 2:334304/147‑1 (MQ=255) aCGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGCGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTc > 6:120446/1‑149 (MQ=255) ggCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGa < 5:120446/149‑1 (MQ=255) aTCCTGTTTGGCCCGTTCGCTACGTTCTCGACCAAGTTCCTGTAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTg < 5:191035/148‑1 (MQ=255) ccTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGc > 8:140136/1‑148 (MQ=255) ccTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGc > 3:63530/1‑148 (MQ=255) tGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTgg < 6:123334/129‑1 (MQ=255) tGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTgg > 5:123334/1‑129 (MQ=255) ccGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTAc > 7:56520/1‑129 (MQ=255) ccGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTAc < 8:56520/129‑1 (MQ=255) gCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGt < 5:170035/145‑1 (MQ=255) gCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGt > 6:170035/1‑145 (MQ=255) cGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTGGGTACTGGTCGatac > 3:261146/1‑109 (MQ=255) cGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTGGGTACTGGTCGatac < 4:261146/112‑4 (MQ=255) gACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCt > 5:366317/1‑149 (MQ=255) gACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACAATGACCg < 8:47131/147‑1 (MQ=255) aaGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCg > 6:358766/1‑148 (MQ=255) aGTTCCTGAAGAACTGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCTACCAGTACCCACTTGTCGAGTACCTCGCTGGCCAGCTTATGCTGTCTGACGATGACCGCTTCGaa < 1:92912/149‑1 (MQ=255) gaagaaCGGCTCGTACCCGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCAACTGGTCGAGTACCTCGTTGGCCAGCTGCTGCTGTCTGACGATGACCGCTTCGACGCCCTgc > 8:327262/1‑149 (MQ=255) aaCGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCAc < 6:366317/149‑1 (MQ=255) gTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTcc < 5:358766/149‑1 (MQ=255) gTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTcc < 5:335719/149‑1 (MQ=255) gTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTc > 3:142137/1‑148 (MQ=255) gTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTc < 4:142137/148‑1 (MQ=255) ccTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCg > 5:186909/1‑148 (MQ=255) cTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGACGCCCTGCGCACCTCCGTCCCGaa > 8:322427/1‑149 (MQ=255) tGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAAc > 7:46491/1‑149 (MQ=255) ctgctgAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGt < 8:298427/100‑1 (MQ=255) ctgctgAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGt > 7:298427/1‑100 (MQ=255) gctgAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaa < 7:242735/147‑1 (MQ=255) gctgAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaa > 8:242735/1‑147 (MQ=255) gcagcaCCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaagaaag > 4:98487/1‑147 (MQ=255) gcagcaCCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAagaaagaa < 8:46491/149‑1 (MQ=255) cagcaCCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaagaaag > 2:357932/1‑146 (MQ=255) caccaccacCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCtt < 7:4128/105‑1 (MQ=255) caccaccacCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCtt > 8:4128/1‑105 (MQ=255) accaccaccCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATg < 3:98487/149‑1 (MQ=255) ccaccaccCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATgg < 7:140136/149‑1 (MQ=255) caccaccCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGgc < 4:63530/149‑1 (MQ=255) caccaccCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGgc > 8:334200/1‑149 (MQ=255) accaccCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGgcg > 3:95902/1‑149 (MQ=255) ccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGTTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtgt > 6:133181/1‑149 (MQ=255) ccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaaga > 3:109885/1‑131 (MQ=255) ccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaaga < 4:109885/131‑1 (MQ=255) acaACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTgg > 5:328242/1‑149 (MQ=255) aaCGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCa < 6:328242/149‑1 (MQ=255) aaCGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCa < 6:186909/149‑1 (MQ=255) gACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGt < 6:81512/38‑1 (MQ=255) gACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGt > 5:81512/1‑38 (MQ=255) ccAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg > 8:89721/1‑149 (MQ=255) ccAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg < 1:357932/149‑1 (MQ=255) cAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGc < 7:334200/149‑1 (MQ=255) tCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAAt < 2:305848/108‑1 (MQ=37) tCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAAt > 1:305848/1‑108 (MQ=37) tCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCaaa > 2:259518/1‑149 (MQ=255) aCCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTACGAAGCCCTGCGCACCTa < 4:233767/79‑1 (MQ=255) aCCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTACGAAGCCCTGCGCACCTa > 3:233767/1‑79 (MQ=255) ccAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTTGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGt > 5:115968/1‑148 (MQ=255) cccACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatca < 4:130693/134‑1 (MQ=255) cccACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatca > 3:130693/1‑134 (MQ=255) cccACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGcc > 8:118545/1‑148 (MQ=255) ccACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGcc > 3:80428/1‑147 (MQ=255) | GACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCC > NC_002947/1428836‑1429123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |