Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,456,488 C→T 7.9% M165I (ATG→ATA)  PP_1274 ← short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,456,4880CT 7.9% 182.8 / 4.3 63M165I (ATG→ATA) PP_1274short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (30/28);  new base T (3/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGACGTTGGCTTGTCCTGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTCACCACCAGGCCGCGT  >  NC_002947/1456340‑1456632
                                                                                                                                                    |                                                                                                                                                
ccgACGTTGGCTTGTCCTGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTcc                                                                                                                                                  <  8:261061/149‑1 (MQ=255)
        ggCTTGTCCTGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTAccc                                                                                                                                           <  4:187568/148‑1 (MQ=255)
         gCTTGTCCTGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCg                                                                                                                                          >  4:105238/1‑148 (MQ=255)
          cTTGTCCTGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTa                                                                                                                                        <  4:207743/149‑1 (MQ=255)
               ccTGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACAcc                                                                                                                                    >  5:288665/1‑148 (MQ=255)
                 tGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGaa                                                                                                                                 >  8:222196/1‑149 (MQ=255)
                 tGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGaa                                                                                                                                 >  6:177970/1‑149 (MQ=255)
                 tGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGaa                                                                                                                                 >  4:85906/1‑149 (MQ=255)
                 tGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGaa                                                                                                                                 >  4:151651/1‑149 (MQ=255)
                     aTGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGgc                                                                                                                             >  7:212699/1‑149 (MQ=255)
                        cccgcgcccgcgCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGcc                                                                                                                          <  3:105238/149‑1 (MQ=255)
                         ccgcgcccgcgcCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGgcgc                                                                                                                           >  7:105232/1‑147 (MQ=255)
                         ccgcgcccgcgcCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGcct                                                                                                                         >  8:149390/1‑148 (MQ=255)
                          cgcgcccgcgccTGCACATGCTCCCGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAg                                                                                                                        >  4:92884/1‑149 (MQ=255)
                                  cgccTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGTCGCCAGCACCTGCTGGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGgc                                                                                                                <  7:313175/149‑1 (MQ=255)
                                    ccTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGgcgc                                                                                                              <  2:145160/149‑1 (MQ=255)
                                      ggcctttGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTTCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGgcgc                                                                                                              >  6:268826/7‑147 (MQ=255)
                                         acaTGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAgg                                                                                                          >  1:178570/1‑148 (MQ=255)
                                           aTGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCAt                                                                                                       <  5:115500/149‑1 (MQ=255)
                                             gCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCTATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCg                                                                                                     >  4:125935/1‑149 (MQ=255)
                                             gCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTac                                                                                                                                       <  6:60509/115‑1 (MQ=255)
                                             gCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTac                                                                                                                                       >  5:60509/1‑115 (MQ=255)
                                             gCTCACACAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCg                                                                                                     >  2:200215/1‑149 (MQ=255)
                                             gCTAACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCTATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCg                                                                                                     >  4:130981/1‑149 (MQ=255)
                                                  cGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAg                                                                                                <  2:5965/149‑1 (MQ=255)
                                                       aGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCAt                                                                                           <  6:288665/149‑1 (MQ=255)
                                                         gCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATg                                                                                          >  1:7505/1‑148 (MQ=255)
                                                         gCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGc                                                                                         <  3:85906/149‑1 (MQ=255)
                                                           caccacGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGca                                                                                        >  3:162840/1‑148 (MQ=255)
                                                                   cGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCt                                                                               >  4:65369/1‑149 (MQ=255)
                                                                           ccgccAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAg                                                                          <  4:70641/146‑1 (MQ=255)
                                                                           ccgccAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCACAGGGCATGCACAGCCGCCTTCGAGgcg                                                                       <  1:200215/149‑1 (MQ=255)
                                                                              ccAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCAAGCACAGCCGCCTTCGAGGCGCAg                                                                    >  6:62956/1‑149 (MQ=255)
                                                                               cAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCAAGCACAGCCGCCTTCGAGGCGCAGt                                                                   <  5:62956/149‑1 (MQ=255)
                                                                                   aCCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTAcgc                                                               >  3:182666/1‑149 (MQ=255)
                                                                                    ccTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTAcgcg                                                              >  8:139882/1‑149 (MQ=255)
                                                                                     cTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACgcgc                                                             >  5:138747/1‑149 (MQ=255)
                                                                                     cTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACgcgc                                                             >  3:89573/1‑149 (MQ=255)
                                                                                       gCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACgcgc                                                             >  7:83896/1‑147 (MQ=255)
                                                                                            gCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGaa                                                      <  7:222196/149‑1 (MQ=255)
                                                                                             ccTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAAt                                                     <  2:178570/149‑1 (MQ=255)
                                                                                                ggCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGc                                                  >  5:254752/1‑149 (MQ=255)
                                                                                                  cgcTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGt                                                <  6:254752/149‑1 (MQ=255)
                                                                                                    cTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGa                                              <  3:92884/149‑1 (MQ=255)
                                                                                                       ggCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCa                                           <  6:138747/149‑1 (MQ=255)
                                                                                                          aTTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTa                                        <  8:16626/149‑1 (MQ=255)
                                                                                                               tGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCTAGGCACAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGccc                                   >  2:57585/1‑149 (MQ=255)
                                                                                                                 gCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCg                                  <  3:65369/148‑1 (MQ=255)
                                                                                                                 gCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGa                                 <  7:205955/149‑1 (MQ=255)
                                                                                                                                aTTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCa                                                                                                                         >  7:116990/1‑46 (MQ=255)
                                                                                                                                aTTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCa                                                                                                                         <  8:116990/46‑1 (MQ=255)
                                                                                                                                aTTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATAtt                  <  8:212699/149‑1 (MQ=255)
                                                                                                                                 ttGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTc                 <  5:177970/149‑1 (MQ=255)
                                                                                                                                    cgcCCGGCTGTACTTCTATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTcac               <  3:130981/148‑1 (MQ=255)
                                                                                                                                    cgcCCGGCTGTACTTCTATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTcac               <  3:125935/148‑1 (MQ=255)
                                                                                                                                    cgcCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTcac               <  4:162840/148‑1 (MQ=255)
                                                                                                                                      cccGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTcaccac            >  6:201976/1‑149 (MQ=255)
                                                                                                                                      cccGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTcaccac            >  5:71545/1‑149 (MQ=255)
                                                                                                                                        cGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTCAccacca          <  2:7505/149‑1 (MQ=255)
                                                                                                                                                cTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTCACCACCAGGCCGCGt  <  4:89573/149‑1 (MQ=255)
                                                                                                                                                 ttCTATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTCACCACCAGGCCGCGt  >  5:239639/1‑148 (MQ=255)
                                                                                                                                                 ttCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGa                                 <  4:330613/117‑1 (MQ=255)
                                                                                                                                                 ttCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGa                                 >  3:330613/1‑117 (MQ=255)
                                                                                                                                                 ttCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTCACCACCAGGCCGCGt  >  4:164404/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCGACGTTGGCTTGTCCTGCGATGCCCGCGCCCGCGCCTGCACATGCTCACGCAAAGGCCACCACGCCGAGTCTGCCGCCAGCACCTGCTCGGCCTGGCGCTGGGCATTGCTGGCGAACTGCGAGGCGATTGCGCCCGGCTGTACTTCCATTACCCGTACACCGAACGGCGCCAGTTCCAGGCGCAAGGCATCGCTCAGGGCATGCACAGCCGCCTTCGAGGCGCAGTACGCGCCAGCGAATGGCGTGACCAGTACGCCCGATACACTGCCGATATTCACCACCAGGCCGCGT  >  NC_002947/1456340‑1456632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑