Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 85,074 A→G 14.0% intergenic (+52/+78) aroE → / ← PP_0075 shikimate 5‑dehydrogenase/choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294785,0740AG14.0% 84.4 / 6.7 37intergenic (+52/+78)aroE/PP_0075shikimate 5‑dehydrogenase/choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base A (15/16);  new base G (3/2);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

GGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTG  >  NC_002947/84932‑85217
                                                                                                                                              |                                                                                                                                                
ggcagCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑cg                                                                                                                                           <  1:233381/149‑1 (MQ=255)
   agCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTACCACAAAGTA‑cgtcg                                                                                                                                        >  1:1714/1‑149 (MQ=255)
        aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGc                                                                                                                                   <  7:210966/149‑1 (MQ=255)
              tcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACg                                                                                                                                    <  2:288760/142‑1 (MQ=255)
              tcttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGATGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACg                                                                                                                                    >  1:288760/1‑142 (MQ=255)
               cttTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCct                                                                                                                            >  5:346385/1‑149 (MQ=255)
                  tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctc                                                                                                                           <  5:184516/147‑1 (MQ=255)
                  tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctc                                                                                                                           <  4:160081/147‑1 (MQ=255)
                        gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg                                                                                                                       <  5:214303/145‑1 (MQ=255)
                        gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg                                                                                                                       <  7:282581/145‑1 (MQ=255)
                        gcggggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg                                                                                                                       <  5:219522/145‑1 (MQ=255)
                             gcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCCCTTTGCGGCCCAATCGCGACGCAAGGCCGTTACAACAAAGTA‑CCTCGTACGCCGCTCCTCATTGGGAgccgcc                                                                                                              >  8:144556/1‑149 (MQ=255)
                               gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCtt                                                                                                            <  7:144556/149‑1 (MQ=255)
                               gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCtt                                                                                                            <  5:8884/149‑1 (MQ=21)
                                             ggCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGCCGCAAGGCCGTTCCCACACAGTA‑CCTCCTACGCCGGGGCTCAGTGGGAGCGGGCGTGGGGCGCGATGGGc                                                                                              >  2:189188/1‑149 (MQ=255)
                                               cgcCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTg                                                                                            <  6:342210/149‑1 (MQ=255)
                                                       tgggcgcaaCGGCGCCGGCAACTCGCTCCGCGCTGAGCGGGCCGGGGCCGCTTGGCGGCCCGACCGCGACGCCAGGCCGTGCCCACAAAGTA‑CCTCGTACGCCGCTCCTCAGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCa                                                                                     <  5:184312/141‑1 (MQ=255)
                                                                     ccGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGATCCCCCAAAGTA‑CGTCGTACCCCGATCCTCAGTGGGAGCGGCCTTGCCTCGCGATGGGCTGCAAAGCAGAACCAGAAACCCaa                                                                      >  6:103771/1‑149 (MQ=39)
                                                                            ctcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAAt                                                                           >  5:157166/1‑137 (MQ=255)
                                                                            ctcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGGCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAAt                                                                           <  6:157166/137‑1 (MQ=255)
                                                                                           gCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc                                                      >  7:275379/1‑143 (MQ=255)
                                                                                           gCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc                                                      <  8:275379/143‑1 (MQ=255)
                                                                                               gatagggCCGCCAAGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGca                                            >  5:330392/5‑149 (MQ=255)
                                                                                                        gCTTGGCGGCCATATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGCATCCCAACCCCATTCCGCAAACCGGATCGGGCACAACTcccc                                   >  4:256172/1‑147 (MQ=255)
                                                                                                                  cccaTCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc                         >  2:104283/4‑149 (MQ=17)
                                                                                                                  ccTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc                         >  3:296073/1‑149 (MQ=255)
                                                                                                                   ccaTCGCGACGCAAGGCCGCTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCa                           >  3:78503/3‑146 (MQ=255)
                                                                                                                   ccaTCGCGACGCAAGGCCGCTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCa                           <  4:78503/144‑1 (MQ=255)
                                                                                                                        gcgACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTg                    >  6:317638/1‑148 (MQ=255)
                                                                                                                         cgACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc                   >  5:322090/1‑148 (MQ=255)
                                                                                                                               aaGGCCGTTCCCACAATGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcct            <  5:103771/149‑1 (MQ=255)
                                                                                                                                 ggCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATACCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCg          <  2:239445/149‑1 (MQ=255)
                                                                                                                                  gCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcctc           >  2:77986/1‑147 (MQ=255)
                                                                                                                                  gCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATAGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGa         >  2:97975/1‑149 (MQ=21)
                                                                                                                                      ctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATc                                                 >  8:70928/2‑105 (MQ=17)
                                                                                                                                      ctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATc                                                 <  7:70928/104‑1 (MQ=17)
                                                                                                                                         ccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTg  >  4:129832/1‑149 (MQ=255)
                                                                                                                                             aaaGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTc                                                           <  6:23290/88‑1 (MQ=255)
                                                                                                                                             aaaGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTc                                                           >  5:23290/1‑88 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GGCAGCTGAAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTG  >  NC_002947/84932‑85217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑