Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,483,878 C→G 7.0% intergenic (+23/‑45) yhdW → / → yhdX amino acid ABC transporter‑binding protein YhdW/amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,483,8780CG 7.0% 172.8 / 2.7 56intergenic (+23/‑45)yhdW/yhdXamino acid ABC transporter‑binding protein YhdW/amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base G (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGTAACTATGGCGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTCCAGATCCTCACGATCGTCTTCGTGGTC  >  NC_002947/1483734‑1484018
                                                                                                                                                 |                                                                                                                                             
tGGGTAACTATGGCGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCccgc                                                                                                                                           <  5:60118/149‑1 (MQ=255)
     aaCTATGGCGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCccgccgccg                                                                                                                                      >  8:90244/1‑149 (MQ=255)
       cTATGGCGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCa                                                                                                                                    <  3:261343/149‑1 (MQ=255)
         aTGGCGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAgg                                                                                                                                  >  7:146781/1‑149 (MQ=255)
         aTGGCGAAGGGTTCGAGAACAACCTCGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCGGGATGCCGCCGCAgg                                                                                                                                  <  3:97554/149‑1 (MQ=255)
            gCGAAGTTTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCTT‑GAACGACCTGTGGAAAAAAGTCTGCATCCAGTACGCGCCTCCAGTGAGCTGATGGCTGCACCCTGCGGCCGCCTCCCGCCGCCGCATGCTg                                                                                                                               >  6:42539/1‑149 (MQ=255)
            gCGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCGGGATGCCGCCGCAgg                                                                                                                                  >  5:4461/1‑146 (MQ=255)
             cGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGt                                                                                                                              >  1:16484/1‑149 (MQ=255)
              gAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCATGGAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGt                                                                                                                              >  1:277868/1‑149 (MQ=255)
              gAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGtt                                                                                                                             <  7:90244/149‑1 (MQ=255)
               aaGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTc                                                                                                                            <  7:209550/149‑1 (MQ=255)
                    ttCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GGACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATc                                                                                                                       >  5:103783/1‑149 (MQ=255)
                      cGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATccc                                                                                                                     >  8:196496/1‑149 (MQ=255)
                       gagaAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATccc                                                                                                                     >  1:353923/1‑148 (MQ=255)
                        agaagaACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTa                                                                                                                   >  8:53614/1‑149 (MQ=255)
                             aaCCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTc                                                                                                              >  1:199596/1‑149 (MQ=255)
                               ccTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GTACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCTCAGGCTGTTCGAATCCCTACTGTCCg                                                                                                            <  1:192633/149‑1 (MQ=255)
                                   ggCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCgggg                                                                                                         <  2:199596/148‑1 (MQ=255)
                                             cTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCACGCCGCCGCAGGCTGTTCGAATACCTACTGTCCGGGGC‑ACTTCATGc                                                                                              >  2:175873/1‑148 (MQ=255)
                                                  ttGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCggg                                                                                                          <  8:132306/132‑1 (MQ=255)
                                                  ttGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCggg                                                                                                          >  7:132306/1‑132 (MQ=255)
                                                    gAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAaaatca                                                                                       <  2:16484/148‑1 (MQ=255)
                                                     aaGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAaaatcaaa                                                                                     <  6:69127/149‑1 (MQ=255)
                                                       gATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAaaatcaaatc                                                                                   >  6:280398/1‑149 (MQ=255)
                                                               gTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCAcc                                                                           <  2:353923/149‑1 (MQ=255)
                                                                tgccAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTCCGGCCGCATCCCGCCGCCCCAGGCTGATCGAATCCCTACTGTCCCGGGC‑ACATCATCAAAAATAAAATACGCGCAccc                                                                          >  8:137824/4‑149 (MQ=255)
                                                                        aaCGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAgggtt                                                                   >  1:132887/1‑147 (MQ=255)
                                                                              cTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTccc                                                             <  7:196496/149‑1 (MQ=255)
                                                                                   gAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGTTTGTCCCTTaa                                                         <  2:132887/148‑1 (MQ=255)
                                                                                    accaccggcgcCCTCCCGTCCCCGCCTCCCGTGCGCTGATGGCGGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTGCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTaa                                                         <  4:294867/142‑1 (MQ=255)
                                                                                    aacaacGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTaa                                                         >  3:294867/1‑147 (MQ=255)
                                                                                    aacaacGGCGGCATCCAGTACGCGACTCCAGTGCGCTGATGTCTGCACCCTGCGGCGGCATCCCGCCGCGGCCGGCTGGTCGACTCCCTACTGTCCCGGGC‑ACTTCATGCAAAATCAAATCGGATAACACAATGGCTTGTCCCTTAAc                                                        >  6:274261/1‑148 (MQ=255)
                                                                                            cggcATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCgt                                               <  2:277868/149‑1 (MQ=255)
                                                                                              gcATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCgtgt                                             <  6:103783/149‑1 (MQ=255)
                                                                                              gcATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCgtgt                                             <  7:137824/149‑1 (MQ=255)
                                                                                                aTCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTgc                                           >  5:310269/1‑149 (MQ=255)
                                                                                                   cAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCAt                                                                                                >  8:189100/1‑93 (MQ=255)
                                                                                                   cAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCAt                                                                                                <  7:189100/93‑1 (MQ=255)
                                                                                                      tACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGt                                     >  5:367356/1‑149 (MQ=255)
                                                                                                       aCGCGGCTGCAGTGCGCTGATGGCTGCACCCTGCGGCTGCATCCCGCAGCCGCAGGGTGTTCTAATCCCTACTGTCCGGTGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGt                                     <  5:42539/148‑1 (MQ=255)
                                                                                                        cgcgCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTg                                    <  6:367356/148‑1 (MQ=255)
                                                                                                         gcgcCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGc                                  <  8:146781/149‑1 (MQ=255)
                                                                                                             cTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCgt                                               >  5:262728/1‑132 (MQ=255)
                                                                                                             cTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCgt                                               <  6:262728/132‑1 (MQ=255)
                                                                                                                cAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTc                             >  4:131553/1‑147 (MQ=255)
                                                                                                                cAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTc                             >  1:337483/1‑147 (MQ=255)
                                                                                                                cAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGACGCAGGCTGTTAGAATCCCTACTGACCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCATGTCCCTTAACGATCCGAGTGTGCGCGAGTGGCTGATc                             >  4:320366/1‑147 (MQ=255)
                                                                                                                 actGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCATGTGCGCGCGTGGCTGTTCCAg                          <  7:53614/147‑1 (MQ=255)
                                                                                                                  gTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTg                                    <  3:60096/138‑1 (MQ=255)
                                                                                                                  gTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTg                                    >  4:60096/1‑138 (MQ=255)
                                                                                                                     cgcTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTCCAGATc                       <  2:337483/148‑1 (MQ=255)
                                                                                                                             gCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTCCAGATCCTCACGATc              <  6:310269/149‑1 (MQ=255)
                                                                                                                               aacagccTGCGGCGGCGGGATGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTcc                            <  5:299850/128‑1 (MQ=39)
                                                                                                                               aacagccTGCGGCGGCGGGATGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTcc                            >  6:299850/6‑133 (MQ=39)
                                                                                                                                          gcggcATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGCAACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTTGCTGTTCCAGATCCTCACGATCGTCTTCGTGGTc  >  1:263242/1‑149 (MQ=255)
                                                                                                                                                tCCCGCCGCCGCAGGCTTTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTACCTGAACGATCCGCGTGTGCGCGCGTGGCTGTTCCAGATCCTCACGATTGt            >  2:123210/1‑132 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TGGGTAACTATGGCGAAGTGTTCGAGAAGAACCTGGGCCAGAGCACTGACTTGAAGATCGACCGTGGCAT‑GAACGCCCTGTGGAACAACGGCGGCATCCAGTACGCGCCTCCAGTGCGCTGATGGCTGCACCCTGCGGCGGCATCCCGCCGCCGCAGGCTGTTCGAATCCCTACTGTCCGGGGC‑ACTTCATGCAAAATCAAATCGGCGCACCCAAGGGCTTGTCCCTTAACGATCCGCGTGTGCGCGCGTGGCTGTTCCAGATCCTCACGATCGTCTTCGTGGTC  >  NC_002947/1483734‑1484018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑