Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,486,293 G→T 8.0% G25V (GGC→GTC)  yhdZ → amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,486,2930GT 8.0% 146.2 / 3.9 50G25V (GGC→GTC) yhdZamino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (27/19);  new base T (2/2);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

GTTTCGGTATGTCCCGCTACTCCATGCATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGAGCACCAGCAAG  >  NC_002947/1486145‑1486424
                                                                                                                                                    |                                                                                                                                   
gTTTCGGTATGTCCCGCTACTCCATGCATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACgg                                                                                                                                     >  4:134687/1‑149 (MQ=255)
 tttCAGTATGTCCCGCTACTCCATGCATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGTAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGc                                                                                                                                    >  8:126865/1‑149 (MQ=255)
      gTATGTCCCGCTACTACATGAATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGACTTTCTAAATGAGTGAAGCGATCAAGCAGCCTGCTGGACCCGAAGGCATCATCCAGATGCAGGGGGTCAACAAGTGGTACTGCAAGt                                                                                                                                >  4:186193/1‑148 (MQ=255)
           tCCCGCTACTCCATGCATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCAt                                                                                                                           <  5:212583/148‑1 (MQ=255)
             ccGCTACTCCATGCATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCAtgtg                                                                                                                        >  4:171757/1‑149 (MQ=255)
                cTACTCCATGCATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGTAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTc                                                                                                                     <  7:126865/149‑1 (MQ=255)
                                gAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAAc                                                                                                         <  2:36903/145‑1 (MQ=255)
                                   cgcAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACg                                                                                                  >  4:31714/1‑149 (MQ=255)
                                          tGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTgcgc                                                                                             >  5:150135/1‑147 (MQ=255)
                                            gACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAgg                                                                                         >  7:74257/1‑149 (MQ=255)
                                                   gCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAAc                                                                                   <  3:171757/148‑1 (MQ=255)
                                                     cacaAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCa                                                                                >  4:212408/1‑149 (MQ=255)
                                                             ttAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCtg                                                                        >  5:203824/1‑149 (MQ=255)
                                                                   gTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCggg                                                                  <  3:134687/149‑1 (MQ=255)
                                                                    tttCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGc                                                                 >  1:206374/1‑149 (MQ=255)
                                                                               gTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAggg                                                                                        >  2:104152/1‑115 (MQ=255)
                                                                                tGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCt                                                                                                       >  3:14510/1‑99 (MQ=255)
                                                                                tGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCt                                                                                                       <  4:14510/99‑1 (MQ=255)
                                                                                   aGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGc                                                   >  1:232950/1‑148 (MQ=255)
                                                                                       aTCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCACCCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTc                                              <  3:186193/149‑1 (MQ=255)
                                                                                          aaGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCCCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTccac                                           >  1:294949/1‑149 (MQ=255)
                                                                                            gcagcCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCGAGGTCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAg                                                <  3:290895/142‑1 (MQ=255)
                                                                                            gcagcCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAg                                                >  8:127285/1‑142 (MQ=255)
                                                                                            gcagcCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAg                                                <  7:127285/142‑1 (MQ=255)
                                                                                            gcagcCTGCTGGCCCCGAAGGCATCATCCACATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGAGGGCCGTCCGGCTCGGGCAAg                                                >  4:290895/1‑142 (MQ=255)
                                                                                               gcCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTccaccacca                                      <  2:206374/149‑1 (MQ=255)
                                                                                                   gctgGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCg                                  >  2:90410/1‑149 (MQ=255)
                                                                                                     tgGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGTCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGc                                                                 >  3:274712/1‑116 (MQ=255)
                                                                                                     tgGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGTCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGc                                                                 >  3:269871/1‑116 (MQ=255)
                                                                                                     tgGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGTCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGc                                                                 <  4:274712/116‑1 (MQ=255)
                                                                                                     tgGACCCTAAGTCATGATCCATATGCATGGTGTGAACAATTGGTTCGTACATTTCCATGTTGTCAAGGACATCAACCTGTACGTGCGCCAGGGCGAACGCATCGTGCTGTGCggtc                                                                 <  4:269871/116‑3 (MQ=19)
                                                                                                      ggCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTg                               >  1:338771/1‑149 (MQ=255)
                                                                                                        ccccGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGcc                             <  2:294949/149‑1 (MQ=255)
                                                                                                         cccGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCt                            >  8:23860/1‑149 (MQ=255)
                                                                                                          ccGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCt                            >  2:310984/1‑148 (MQ=255)
                                                                                                                gCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGc                     <  7:23860/149‑1 (MQ=255)
                                                                                                                gCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCAGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGc                     >  7:93107/1‑149 (MQ=255)
                                                                                                                  atcatcCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCt                   >  3:223060/1‑149 (MQ=255)
                                                                                                                   tcatcCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTg                  >  2:148545/1‑149 (MQ=255)
                                                                                                                     atcCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTgg                 >  8:175788/1‑148 (MQ=255)
                                                                                                                     atcCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTgg                 <  1:322040/148‑1 (MQ=255)
                                                                                                                      tcCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTgg                 >  8:162511/1‑147 (MQ=255)
                                                                                                                        cAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAaga             <  4:223060/149‑1 (MQ=255)
                                                                                                                        cAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAag              <  2:232950/148‑1 (MQ=255)
                                                                                                                           aTGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCCCCACCATCCGCTGCCTTAGCCGCGTGGAGGAGCa          >  4:32418/1‑149 (MQ=255)
                                                                                                                           aTGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTCTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGATCa          >  8:73625/1‑149 (MQ=255)
                                                                                                                            tGCAGGGTGTGAACAAGTGGTAGGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGAGCAc         <  8:74257/149‑1 (MQ=255)
                                                                                                                            tGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGAGCAc         <  3:212408/149‑1 (MQ=255)
                                                                                                                                   tgtgAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGAGCACCAGCAAg  <  3:32418/149‑1 (MQ=255)
                                                                                                                                      gAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGAGCACCAGCaa   >  4:85833/1‑145 (MQ=255)
                                                                                                                                      gAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGAGCACCAGCaa   <  3:85833/145‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                   
GTTTCGGTATGTCCCGCTACTCCATGCATCTGGAGCGCAAGCTGGACACTGGCCACAAGCGTTAGGAGTTTCGAAATGAGTGAAGCGATCAAGCAGCCTGCTGGCCCCGAAGGCATCATCCAGATGCAGGGTGTGAACAAGTGGTACGGCCAGTTCCATGTGCTCAAGGACATCAACCTGAACGTGCGCCAGGGCGAACGCATCGTGCTGTGCGGGCCGTCCGGCTCGGGCAAGTCCACCACCATCCGCTGCCTTAACCGCCTGGAAGAGCACCAGCAAG  >  NC_002947/1486145‑1486424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑