Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,488,066 T→A 6.4% Y86F (TAC→TTC)  degS ← serine endoprotease DegS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,488,0660TA 6.4% 191.1 / 2.3 62Y86F (TAC→TTC) degSserine endoprotease DegS
Reads supporting (aligned to +/- strand):  ref base T (29/29);  new base A (2/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTGGATGA  >  NC_002947/1487921‑1488210
                                                                                                                                                 |                                                                                                                                                
cagGTAGCCCTCTGGGCTCATGGTTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGTGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTACACCACCAAGGTGGTGAAc                                                                                                                                                >  2:216992/1‑148 (MQ=255)
cagGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACa                                                                                                                                               <  2:291575/149‑1 (MQ=255)
      gCCCTCTGGGCTCATGGTTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGTGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTACACCACCAAGGTGGTGAACaaaagc                                                                                                                                          <  1:216992/148‑6 (MQ=255)
         ctctGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCa                                                                                                                                      >  4:325289/1‑149 (MQ=255)
               gCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCAccgcc                                                                                                                                 <  2:347522/148‑1 (MQ=255)
                 tCATGATTACCGCGGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGATGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGAGCTTTTCTTCACCACGTTGGTGGTGTACAGGTTGGACACCGCCggg                                                                                                                              >  6:204387/1‑149 (MQ=255)
                     gATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcgg                                                                                                                          >  1:220626/1‑149 (MQ=255)
                      aTTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGggcggc                                                                                                                         >  4:300949/1‑149 (MQ=255)
                        tACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGGTGGTGAAc                                                                                                                                                <  8:198960/124‑1 (MQ=255)
                        tACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCAAGGTGGTGAAc                                                                                                                                                >  7:198960/1‑124 (MQ=255)
                          ccgccgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAgg                                                                                                                     >  3:278397/1‑149 (MQ=255)
                           cgccgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAggg                                                                                                                    >  5:353464/1‑149 (MQ=255)
                           cgccgAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAggg                                                                                                                    >  1:296510/1‑149 (MQ=255)
                                     aGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtc                                                                                                          >  4:209205/1‑149 (MQ=255)
                                       gCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCAcggcgtcgg                                                                                                        <  4:278397/149‑1 (MQ=255)
                                          tGATTCCCAGCGGCGCTGTATGGGCAGGTTATCTCCGAAGAAGCGGAGGAACTGCGGGTCTACGAACAGCCCGTCGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGCCGGCCAGGGTCAcggcgtcgtag                                                                                                      >  8:40758/1‑145 (MQ=255)
                                             ttCCCAGCGGCGCTGTTTGGGCAGGTAATCGCCGAAGAAGCGGCGGAACTGCGGGTATTCGAACAGCTGGTGGCCGCTTTTGTTCACCACCTTGGTGGTGTAAAGGTTGGCCACCGCCGGCGCGTCCAGGCTCACGGCGTAGGAGTAc                                                                                                   >  5:99309/1‑148 (MQ=255)
                                                    cggcgCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAgg                                                                                                                     <  7:300855/123‑1 (MQ=255)
                                                    cggcgCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAgg                                                                                                                     >  8:300855/1‑123 (MQ=255)
                                                      gcgcTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGc                                                                                         >  8:320238/1‑149 (MQ=255)
                                                                 gCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCtt                                                                                      >  2:171659/1‑141 (MQ=255)
                                                                 gCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCtt                                                                                      <  1:171659/141‑1 (MQ=255)
                                                                 gCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATc                                                                              >  1:167189/1‑149 (MQ=255)
                                                                    ggTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                           <  3:325289/149‑1 (MQ=255)
                                                                     gTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCggg                                                                          >  6:320764/1‑149 (MQ=255)
                                                                     gTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCggg                                                                          <  3:300949/149‑1 (MQ=255)
                                                                     gTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                           >  1:250511/1‑148 (MQ=255)
                                                                     gTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCgg                                                                           <  2:250511/148‑1 (MQ=255)
                                                                      ttATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCggg                                                                          >  3:179863/1‑148 (MQ=255)
                                                                         tCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGt                                                                      <  3:209205/149‑1 (MQ=255)
                                                                         tCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGt                                                                      >  4:158111/1‑149 (MQ=255)
                                                                              gaagaaGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCtgtg                                                                 <  8:139207/149‑1 (MQ=255)
                                                                                agaagCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGCTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGgc                                                               <  2:108796/149‑1 (MQ=255)
                                                                                    gcggcgGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCtt                                                           <  5:320764/149‑1 (MQ=255)
                                                                                       gcggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtgct                                                        >  5:182373/1‑149 (MQ=255)
                                                                                         ggAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTtgctgc                                                      <  2:220626/149‑1 (MQ=255)
                                                                                              tGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGtt                                                 >  2:179675/1‑149 (MQ=255)
                                                                                               gCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGtt                                                 >  6:211570/1‑148 (MQ=255)
                                                                                                cGGGTCTTCGAACAGGGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACTGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAgg                                                   <  5:204387/145‑1 (MQ=255)
                                                                                                 aacacTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACGCCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGAc                                              <  2:42211/145‑1 (MQ=255)
                                                                                                   gTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAg                                                    <  4:134068/141‑1 (MQ=255)
                                                                                                   gTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAg                                                    >  3:134068/1‑141 (MQ=255)
                                                                                                      ttCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCct                                         >  6:213163/1‑149 (MQ=255)
                                                                                                            cAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCctggctgg                                   <  2:161701/149‑1 (MQ=255)
                                                                                                               cGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTggg                                  >  6:67098/1‑147 (MQ=255)
                                                                                                                       cgcTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGtt                                                 <  2:125256/124‑1 (MQ=255)
                                                                                                                       cgcTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGtt                                                 >  1:125256/1‑124 (MQ=255)
                                                                                                                       cgcTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGAcc                        >  4:257375/1‑149 (MQ=255)
                                                                                                                              gTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGc                  <  7:240453/148‑1 (MQ=255)
                                                                                                                              gTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGc                  <  3:158111/148‑1 (MQ=255)
                                                                                                                               ttCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCgg                <  1:179675/149‑1 (MQ=255)
                                                                                                                                tCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCgg                >  2:239816/1‑148 (MQ=255)
                                                                                                                                 caccacCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGa               >  7:45263/1‑148 (MQ=255)
                                                                                                                                  accaccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaaa             <  7:40758/149‑1 (MQ=255)
                                                                                                                                  accaccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaa              >  5:97164/1‑148 (MQ=255)
                                                                                                                                  accaccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGaa              <  1:239816/148‑1 (MQ=255)
                                                                                                                                      ccTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGc         <  5:213163/149‑1 (MQ=255)
                                                                                                                                       cTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt        <  6:182373/149‑1 (MQ=255)
                                                                                                                                       cTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt        <  4:179863/149‑1 (MQ=255)
                                                                                                                                       cTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCt        <  2:167189/149‑1 (MQ=255)
                                                                                                                                          ggtggtGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTg       <  3:257375/147‑1 (MQ=255)
                                                                                                                                             ggtgTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTGgatga  <  2:296510/149‑1 (MQ=255)
                                                                                                                                              gtgtACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTggg                                  >  5:241557/1‑116 (MQ=255)
                                                                                                                                              gtgtACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTggg                                  <  6:241557/116‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGGTAGCCCTCTGGGCTCATGATTACCGCCGAACCCAGGCTTGATTCCCAGCGGCGCTGTTTGGGCAGGTTATCGCCGAAGAAGCGGCGGAACTGCGGGTCTTCGAACAGCGGGTGGGCGCTTTTGTTCACCACCTTGGTGGTGTACAGGTTGGCCACCGCCGGGGCGGCCAGGGTCACGGCGTCGGCGTACGACACCGGGCCTTGCATGATCCGGGTAGTCTGTGGCGCTTGCTGCAGGTTGACGTCCTGGCTGGGCAGCCCGACCCATTGCGGAAAACGCTGGATGA  >  NC_002947/1487921‑1488210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑