| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,505,541 | T→A | 12.3% | V218D (GTC→GAC) | petB → | cytochrome b |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,505,541 | 0 | T | A | 12.3% | 99.4 / 3.6 | 39 | V218D (GTC→GAC) | petB | cytochrome b |
| Reads supporting (aligned to +/- strand): ref base T (21/13); new base A (2/3); total (23/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGG > NC_002947/1505397‑1505684 | ttCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGAc < 1:164166/149‑1 (MQ=255) tgACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCaggg < 6:312258/149‑4 (MQ=255) cTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCag > 1:216261/1‑139 (MQ=255) cTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCag < 2:216261/140‑2 (MQ=255) aTCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCagg > 7:53933/1‑136 (MQ=255) aTCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGTCGACGCCATCagg < 8:53933/138‑3 (MQ=255) tGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCg > 1:38940/1‑149 (MQ=255) aaCCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCt > 4:217191/1‑149 (MQ=255) ccGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTg > 4:172924/1‑148 (MQ=255) cttcttCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATACTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCATGATGGCGTCGACATCAAGAAGAAAAAGGAc > 3:146529/1‑127 (MQ=255) cttcGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTa > 2:180761/1‑149 (MQ=255) gCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCaag > 8:209597/1‑108 (MQ=255) gCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCaag < 7:209597/108‑1 (MQ=255) cccTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCg > 8:52824/1‑149 (MQ=255) cATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTcc < 1:180761/148‑1 (MQ=255) cccTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGtt < 8:324762/136‑1 (MQ=255) cccTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGtt > 7:324762/1‑136 (MQ=255) tGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACAcc > 1:317984/1‑149 (MQ=255) cAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTc < 3:290761/111‑1 (MQ=255) cAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTc > 4:290761/1‑111 (MQ=255) cAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGtt < 3:217191/149‑1 (MQ=255) tcGGCCTGCTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCATGATGACGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTc > 6:349992/1‑149 (MQ=255) gCCTGGTTGTATTCCACATCCTCGCCTTGCACGAAGTGGGATCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGc < 8:274148/149‑1 (MQ=255) cTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTCCCTGTTCGTGTTCt > 1:321426/1‑149 (MQ=255) gCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTg < 2:38940/147‑1 (MQ=255) aCGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGc < 4:330630/69‑1 (MQ=255) aCGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGc > 3:330630/1‑69 (MQ=255) tGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCgtggtg > 3:239394/1‑149 (MQ=255) ggTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGATATTGTCGGCGTGGTGGTGTTCCTGt > 1:63477/1‑127 (MQ=255) ggTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGATATTGTCGGCGTGGTGGTGTTCCTGt < 2:63477/127‑1 (MQ=255) ttCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGACGGGGTGttct > 8:333848/1‑149 (MQ=255) aacaacCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttcttctt > 4:264940/1‑149 (MQ=255) ccTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTg > 3:293146/1‑148 (MQ=255) tGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCgtg < 4:245046/130‑1 (MQ=255) tGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCgtg > 3:245046/1‑130 (MQ=255) ggCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtggtgg < 8:103642/100‑1 (MQ=255) ggCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCgtggtgg > 7:103642/1‑100 (MQ=255) ggCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATgg < 4:293146/148‑1 (MQ=255) gTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGGTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATGGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttg > 7:54511/1‑128 (MQ=255) ctCGACATCAAGAAGAAAAAGCACGAAAACGGTATTCCGCTGTATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGttc < 8:54511/128‑1 (MQ=255) | TTCGTGGTGACTACCTGATCTCGGGCATCACCCTGAACCGCTTCTTCGCCCTGCATGTGGTTGCCCTGCCAATCGTGATTCTCGGCCTGGTGGTATTGCACATCCTCGCCTTGCACGAAGTGGGTTCGAACAACCCTGATGGCGTCGACATCAAGAAGAAAAAGGACGAAAACGGTATTCCGCTGGATGGTATTCCGTTCCACCCGTACTACACCGTTAAAGACATTGTCGGCGTGGTGGTGTTCCTGTTCGTGTTCTGCGCCGTGGTGTTCTTCTTCCCTGAAATGG > NC_002947/1505397‑1505684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |