Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,509,663 C→A 7.0% L30L (CTG→CTT) ‡ PP_1324 ← endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,509,6630CA 7.0% 180.0 / 4.0 57L30L (CTG→CTT) ‡PP_1324endonuclease
Reads supporting (aligned to +/- strand):  ref base C (27/26);  new base A (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

ACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTGGCCGCCGCTAAACTCGGCCC  >  NC_002947/1509518‑1509801
                                                                                                                                                 |                                                                                                                                           
aCCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGc                                                                                                                                          <  7:214928/149‑1 (MQ=255)
  cGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCaa                                                                                                                                        <  3:119940/149‑1 (MQ=255)
          cTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGcc                                                                                                                                >  5:155916/1‑149 (MQ=255)
          cTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGc                                                                                                                                 <  5:298101/148‑1 (MQ=255)
           ttGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGccc                                                                                                                               >  4:314420/1‑149 (MQ=255)
           ttGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGccc                                                                                                                               >  4:37418/1‑149 (MQ=255)
              cgcCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCtt                                                                                                                             <  6:15289/148‑1 (MQ=255)
                   gTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCt                                                                                                                       >  7:123780/1‑149 (MQ=255)
                   gTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCt                                                                                                                       >  3:2235/1‑149 (MQ=255)
                           tGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTAc                                                                                                               >  7:139102/1‑149 (MQ=255)
                           tGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTAc                                                                                                               >  5:344786/1‑149 (MQ=255)
                            gTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACt                                                                                                              >  6:340917/1‑149 (MQ=255)
                                   gcgcCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGc                                                                                                       <  6:344786/149‑1 (MQ=255)
                                     gcCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCtt                                                                                                     >  2:176023/1‑149 (MQ=255)
                                        tCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTg                                                                                                    <  2:270257/147‑1 (MQ=255)
                                        tCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTg                                                                                                    >  1:270257/1‑147 (MQ=255)
                                           gAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCTCCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGttt                                                                                               >  2:111746/1‑149 (MQ=255)
                                           gAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGttt                                                                                               >  6:9978/1‑149 (MQ=255)
                                             aGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCt                                                                                             <  3:152331/149‑1 (MQ=255)
                                                ccGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCTTGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCTGGCACGCAACTggcgat                                                                                                                                >  1:272819/1‑106 (MQ=255)
                                                ccGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCTGGCACGCAACTggcgat                                                                                                                                <  2:272819/111‑6 (MQ=255)
                                                ccGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGcc                                                                                          >  2:241332/1‑149 (MQ=255)
                                                  gcgTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGc                                                                                        <  2:329997/149‑1 (MQ=255)
                                                         aCCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCg                                                                                 <  1:257550/149‑1 (MQ=255)
                                                             gTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCAc                                                                             <  5:9978/149‑1 (MQ=255)
                                                               aGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTTCCCCTGAAGGTAATCAAGGGCTTGGGTTTCTGCAGATTTCCCGGCACgg                                                                           >  5:298376/1‑149 (MQ=255)
                                                                      ttCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGc                                                                                                                           <  3:222903/94‑1 (MQ=255)
                                                                      ttCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGc                                                                                                                           >  4:222903/1‑94 (MQ=255)
                                                                      ttCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGc                                                                     >  5:13605/1‑148 (MQ=255)
                                                                        cgacgaATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCg                                                                    <  6:155916/147‑1 (MQ=255)
                                                                             aaTACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCTGGCACGCAACTggcg                                                                                                                                  >  2:326833/1‑77 (MQ=38)
                                                                             aaTACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCTGGCACGCAACTggcg                                                                                                                                  <  1:326833/80‑4 (MQ=38)
                                                                              aTACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGACCGGCACGGGTAGGCGATGCTGCg                                                            <  1:241332/149‑1 (MQ=255)
                                                                                  tactGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTa                                                                        >  7:167779/1‑133 (MQ=255)
                                                                                  tactGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTa                                                                        <  8:167779/133‑1 (MQ=255)
                                                                                   actGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCAt                                                       <  3:37418/149‑1 (MQ=255)
                                                                                    ctGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCAt                                                       >  6:240226/1‑148 (MQ=255)
                                                                                           ggCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCa                                                <  1:289256/148‑1 (MQ=255)
                                                                                           ggCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAc                                               <  8:123780/149‑1 (MQ=255)
                                                                                            gCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑g                                             <  4:2235/149‑1 (MQ=255)
                                                                                             cGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑gg                                            <  1:176023/149‑1 (MQ=255)
                                                                                              gTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑Ggt                                           <  6:13605/149‑1 (MQ=255)
                                                                                                    cATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTcagg                                     <  6:285972/149‑1 (MQ=255)
                                                                                                         ccAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCaggcaggc                                >  7:267164/1‑149 (MQ=255)
                                                                                                              tCAAGCTCACCGCCTTTCCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCAGAAGGTACTCAAGCGCTTGGGTTTCTCCCGCTTGCCCGCCCAGGGTAGCCGATGCTGCGGCCCTCAACGAACTGGTG‑AAGGCACGCGCTTg                           >  4:202671/1‑149 (MQ=255)
                                                                                                                aaGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGAc                         <  8:139102/149‑1 (MQ=255)
                                                                                                                  gCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTcagg                                     >  4:123373/1‑135 (MQ=255)
                                                                                                                  gCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTcagg                                     <  3:123373/135‑1 (MQ=255)
                                                                                                                  gCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCtt                            >  4:37999/1‑144 (MQ=255)
                                                                                                                  gCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCtt                            <  3:37999/144‑1 (MQ=255)
                                                                                                                    tCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTgg                     >  1:295575/1‑149 (MQ=255)
                                                                                                                      acatccTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTGgc                    <  6:298376/144‑1 (MQ=255)
                                                                                                                      aCCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTGgc                    <  5:340917/148‑1 (MQ=255)
                                                                                                                              tGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTGGCCGCCGCTa            >  1:230010/1‑148 (MQ=255)
                                                                                                                                 aTCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTGGCCGCCGCTAAACt        >  2:257336/1‑149 (MQ=255)
                                                                                                                                 aTCGCCACTTGCGTGCCAGCAACTGCAGCCCAAGCCCCTGAAGGTACTCAAGCGCTAGGGTTTCTGCCGCTACCCCGGCACGCGTAGGAGATGCTGCGGCCAACAACGCAC‑GCTCTCAGGCAGCCGATTGACCTGGACGCCGCTAAACt        >  4:175597/1‑149 (MQ=255)
                                                                                                                                         ttGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTGGCCGCCGCTAAACTCGGccc  >  6:14534/1‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
ACCGTTTCTGCTTGCGCCCGTCGATACTGTCGAGAGCGCCTCCGAAGCCCGCGTGCAACCGGTAGCGGACTTCGACGAATACTACTGTATCGGCGTCGAGCATGACCAGATCAAGCTCACCGCCTTTGCATCGCCAGTTGCGTGCCAGCAACTGCAGCCCTTGCCCCTGAAGGTACTCAAGGGCTTGGGTTTCTGCCGCTTGCCCGGCACGGGTAGGCGATGCTGCGGCCATCAACGCAC‑GGTGTCAGGCAGGCGCTTGACCTGGCCGCCGCTAAACTCGGCCC  >  NC_002947/1509518‑1509801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑