Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,528,302	A→G	6.5%	T215A (ACC→GCC)	ftsA →	cell division protein FtsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,528,302	0	A	G	6.5%	175.2 / 2.1	61	T215A (ACC→GCC)	ftsA	cell division protein FtsA
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base G (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

AACGCCGCCCAGAACATCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTG  >  NC_002947/1528155‑1528447                                                                                                                                                     |                                                                                                                                                   aaCGCCGCCCAGAACATCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGcac                                                                                                                                                   >  3:218638/1‑149 (MQ=255)    gccgccCAGAACATCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGcaccac                                                                                                                                                >  7:144879/1‑149 (MQ=255)      cgccCAGAACATCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCg                                                                                                                                              >  1:237578/1‑149 (MQ=255)         ccAGAACATCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACa                                                                                                                                           >  8:27467/1‑149 (MQ=255)                aTCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCAt                                                                                                                                    >  5:300726/1‑149 (MQ=255)                aTCGAGAAGTGTGTGCGCCGCTGCGGCCAGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATAGGTGGCGGCACCACcgccatcgccat                                                                                                                                    >  1:198977/1‑149 (MQ=255)                 tCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATc                                                                                                                                   >  8:307173/1‑149 (MQ=255)                  cGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCt                                                                                                                                  >  2:354309/1‑149 (MQ=255)                   gagaAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCtt                                                                                                                                 >  6:104770/1‑149 (MQ=255)                    agaAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCtt                                                                                                                                 >  2:351012/1‑148 (MQ=255)                      aaGTGTGTGCGCCGCTGCGGGCTGGAAATCGCCGACCTCATCCTCGCGCAGCTGGCTTCGGCCTACTCGGTACTGACGGATGATCAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGTTGGCGGCACCACCGACATCTCCTTCTTCAc                                                                                                                              <  2:198977/149‑1 (MQ=255)                      aaGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCAc                                                                                                                              <  2:237578/149‑1 (MQ=255)                      aaGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCAc                                                                                                                              <  4:168975/149‑1 (MQ=255)                         tgtgtgCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGa                                                                                                                           <  1:354309/149‑1 (MQ=255)                             tgCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCaccacc                                                                                                                                               >  6:300071/1‑125 (MQ=255)                             tgCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCaccacc                                                                                                                                               <  5:300071/125‑1 (MQ=255)                             tgCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAggg                                                                                                                        <  2:100895/148‑1 (MQ=255)                             tgCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGt                                                                                                                       >  5:192511/1‑149 (MQ=255)                               cgccgcTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGc                                                                                                                     >  5:165761/1‑149 (MQ=255)                                    cTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATcc                                                                                                                <  6:180667/149‑1 (MQ=255)                                       cGGCCTGGAAATCGACGACATCATCCGCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGc                                                                                                              <  8:193722/148‑1 (MQ=255)                                         gCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCAATCCGCcac                                                                                                           >  7:143236/1‑149 (MQ=255)                                              gAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACAcggc                                                                                                      >  6:192556/1‑149 (MQ=255)                                                      cgacATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATcc                                                                                              <  7:29714/149‑1 (MQ=255)                                                      cgacATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATcc                                                                                              <  5:192556/149‑1 (MQ=255)                                                      cgacATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATcc                                                                                              <  4:218638/149‑1 (MQ=255)                                                         catcatCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCAACGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGa                                                                                           >  8:265249/1‑149 (MQ=255)                                                             atcCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAGCAGCTGGGCGTGTGCCTGGTGGACATCGGTGGTGCCGCCACCGa                                                                                                                                             <  1:69801/96‑1 (MQ=255)                                                             atcCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGTGCCGCCACCGa                                                                                                                                             >  2:69801/1‑95 (MQ=255)                                                              tcCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCt                                                                                      >  7:212056/1‑149 (MQ=255)                                                                       agcTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGTTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAg                                                                             <  6:192511/149‑1 (MQ=255)                                                                        gcTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGTCATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAgg                                                                            >  2:234152/1‑149 (MQ=255)                                                                         cTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGt                                                                           <  6:165761/149‑1 (MQ=255)                                                                            gCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGa                                                                         <  8:212056/148‑1 (MQ=255)                                                                            gCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGAc                                                                        <  6:300726/149‑1 (MQ=255)                                                                                 ggCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAAc                                                                    <  7:27467/148‑1 (MQ=255)                                                                                    cTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACa                                                               >  5:135051/1‑149 (MQ=255)                                                                                       cTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTg                                                                                     >  8:116424/1‑125 (MQ=255)                                                                                       cTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTg                                                                                     <  7:116424/125‑1 (MQ=255)                                                                                          ggTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCa                                                         >  7:4820/1‑149 (MQ=255)                                                                                             aCTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATgg                                                      >  1:314541/1‑149 (MQ=255)                                                                                                 aCCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGGGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCt                                                  >  6:130320/1‑149 (MQ=255)                                                                                                 aCCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAg                                                                             >  3:168540/1‑123 (MQ=255)                                                                                                 aCCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAg                                                                             <  4:168540/123‑1 (MQ=255)                                                                                                 aCCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGcc                                                    <  7:307173/147‑1 (MQ=255)                                                                                                 aCCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGcc                                                    <  1:351012/147‑1 (MQ=255)                                                                                                           aaaaaG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCg                                        >  5:295369/1‑149 (MQ=255)                                                                                                           aaaaaG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCg                                        >  4:188444/1‑149 (MQ=255)                                                                                                                   gCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGGGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGt                                 <  5:130320/149‑1 (MQ=255)                                                                                                                   gCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGt                                 <  7:265249/149‑1 (MQ=255)                                                                                                                    cTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGt                                 <  6:295369/148‑1 (MQ=255)                                                                                                                       ggCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACGCACATCGCCATGGCCCTGCGTACCCCGACCCAGTAc                               <  1:234152/148‑1 (MQ=255)                                                                                                                        gCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGt                                                                                                                       <  6:107935/59‑1 (MQ=255)                                                                                                                        gCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGt                                                                                                                       >  5:107935/1‑59 (MQ=255)                                                                                                                           tgtgCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCgaa                         <  2:314541/149‑1 (MQ=255)                                                                                                                            gtgCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCg                           >  1:89077/1‑146 (MQ=255)                                                                                                                                    tggACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTAcccc                                         >  2:350257/1‑124 (MQ=255)                                                                                                                                    tggACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTAcccc                                         <  1:350257/124‑1 (MQ=255)                                                                                                                                         gtCGGTGGTGCCGCCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCgaa                         >  6:341059/2‑135 (MQ=255)                                                                                                                                         gtCGGTGGTGCCGCCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCgaa                         <  5:341059/134‑1 (MQ=255)                                                                                                                                               ggcggcACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGc     >  6:275405/1‑149 (MQ=255)                                                                                                                                               ggcggcACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGc     <  3:188444/149‑1 (MQ=255)                                                                                                                                                gcggcACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGc     <  2:89077/148‑1 (MQ=255)                                                                                                                                                  ggcACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCtg  >  8:297039/1‑149 (MQ=255)                                                                                                                                                  ggcACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGGACGCCGAAGAAATCAAGATCCGCTACGCCtt  >  5:193807/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AACGCCGCCCAGAACATCGAGAAGTGTGTGCGCCGCTGCGGCCTGGAAATCGACGACATCATCCTCGAGCAGCTGGCTTCGGCCTACTCGGTACTGACCGATGATGAAAAAG‑AGCTGGGCGTGTGCCTGGTGGACATCGGTGGCGGCACCACCGACATCGCCATCTTCACCGAGGGTGCCATCCGCCACACGGCGGTCATCCCGATTGCTGGCGACCAGGTGACCAACG‑ACATCGCCATGGCCCTGCGTACCCCGACCCAGTACGCCGAAGAAATCAAGATCCGCTACGCCTG  >  NC_002947/1528155‑1528447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑