Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,563,828 A→C 8.0% intergenic (+94/+28) PP_1372 → / ← pitB hypothetical protein/phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,563,8280AC 8.0% 144.4 / 5.2 50intergenic (+94/+28)PP_1372/pitBhypothetical protein/phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base C (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-01

CGGCAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTT  >  NC_002947/1563681‑1563962
                                                                                                                                                   |                                                                                                                                      
cggcAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAg                                                                                                                                       <  4:3661/149‑1 (MQ=255)
  gcAGTTGGTTCGCGGGTTGATGGGTTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCTCCATACCCTTGGTAGCACAAGCCCGCTCCGTCAAAGAACAAGGTGGCGTAGGCATAAATGATATGAAAAATGCGCCAGatct                                                                                                                                     >  2:66983/1‑146 (MQ=255)
        ggTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCtt                                                                                                                               >  4:146804/1‑149 (MQ=255)
            cgcgGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCttcat                                                                                                                            >  1:293317/1‑148 (MQ=255)
               gggTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGTCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCtttttcata                                                                                                                        <  5:51051/149‑8 (MQ=255)
                 gTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGAAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTg                                                                                                                      >  8:115592/1‑149 (MQ=255)
                      tGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGgt                                                                                                                  <  4:468/148‑1 (MQ=255)
                        gggTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTc                                                                                                               <  1:7652/149‑1 (MQ=255)
                        gggTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTc                                                                                                               <  3:146804/149‑1 (MQ=255)
                                   aGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGCCGACCGAGGGCGCCTTCATTCATTGCGgtgt                                                                                                                >  3:248860/1‑137 (MQ=255)
                                    gggggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAAt                                                                                                    <  2:293317/148‑1 (MQ=255)
                                     ggggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATgg                                                                                                  <  1:14667/149‑1 (MQ=255)
                                     ggggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGGACGGATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCg                                                                                                                    >  2:204535/1‑131 (MQ=255)
                                     ggggCAACAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCGCGAGGGCGCCTTCATTCATTGCg                                                                                                                    <  1:204535/131‑1 (MQ=255)
                                       ggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGTCGCCCGAGGGCGCCttca                                                                                                                             >  1:328515/1‑120 (MQ=255)
                                       ggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCttca                                                                                                                             <  2:328515/120‑1 (MQ=255)
                                       ggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCt                                                                                                >  5:98901/1‑149 (MQ=255)
                                        gCAAGAAAAGGTAGTCTTACTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCtt                                                                                               <  8:216967/149‑1 (MQ=255)
                                           aGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCtttttc                                                                                                                           <  3:254546/118‑5 (MQ=255)
                                           aGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCtttttc                                                                                                                           >  4:254546/1‑114 (MQ=255)
                                                     gTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgccagc                                                                                    >  6:232270/1‑147 (MQ=255)
                                                     gTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCAGTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgccagc                                                                                    >  2:207355/1‑147 (MQ=255)
                                                         tCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGaa                                                                               >  4:240250/1‑148 (MQ=255)
                                                          ccTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCttcattc                                                                                                                          >  6:334431/1‑104 (MQ=255)
                                                          ccTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCttcattc                                                                                                                          <  5:334431/104‑1 (MQ=255)
                                                               gccgcgccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGa                                                                                                                                        >  6:320169/1‑85 (MQ=255)
                                                               gccgcgccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGa                                                                                                                                        <  5:320169/85‑1 (MQ=255)
                                                               gccgcgccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAggccg                                                                        <  6:98901/149‑1 (MQ=255)
                                                                     ccATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGgccgc                                                                   >  5:278825/1‑148 (MQ=255)
                                                                           ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGcc                                                             >  2:46344/1‑148 (MQ=255)
                                                                           ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGcc                                                             >  6:11270/1‑148 (MQ=255)
                                                                                    cacaAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggca                                                   <  5:232270/149‑1 (MQ=255)
                                                                                     acaAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCATTGGCTTTGGACgcca                                                                                      >  6:302505/1‑113 (MQ=255)
                                                                                     acaAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggcag                                                  <  5:11270/149‑1 (MQ=255)
                                                                                     acaAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggcag                                                  >  7:15454/1‑149 (MQ=255)
                                                                                      caAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAgg                                                               <  5:155572/135‑1 (MQ=255)
                                                                                      caAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAgg                                                               >  6:155572/1‑135 (MQ=255)
                                                                                         gCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGgcaggcag                                              >  4:27921/1‑149 (MQ=255)
                                                                                                 cGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAg                                        <  3:27921/147‑1 (MQ=255)
                                                                                                  ggCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGAc                                     <  3:123900/149‑1 (MQ=255)
                                                                                                   gCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGAcc                                    >  4:96842/1‑149 (MQ=255)
                                                                                                     aaaGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATc                                                         >  4:259699/1‑126 (MQ=255)
                                                                                                     aaaGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATc                                                         <  3:259699/126‑1 (MQ=255)
                                                                                                          aCAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAg                                                                            >  7:180643/1‑102 (MQ=255)
                                                                                                            aaGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCcagc                           <  1:46344/149‑1 (MQ=255)
                                                                                                              ggTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCcagcag                         >  1:316867/1‑149 (MQ=255)
                                                                                                                           tAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTcaca            <  3:96842/149‑1 (MQ=255)
                                                                                                                               ggCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTgc        >  8:42917/1‑149 (MQ=255)
                                                                                                                                gCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTgcc       <  3:240250/149‑1 (MQ=255)
                                                                                                                                gCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTgcc       <  2:316867/149‑1 (MQ=255)
                                                                                                                                     aatgaaGGCGCCCTCGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCtt  >  4:315371/5‑149 (MQ=255)
                                                                                                                                       aaaaGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGAc                                     >  7:33228/1‑112 (MQ=255)
                                                                                                                                       aaaaGCCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGAc                                     <  8:33228/112‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                      
CGGCAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTT  >  NC_002947/1563681‑1563962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑