Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,575,201 T→A 7.5% intergenic (‑187/+23) pcaP ← / ← galP‑II porin/porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,575,2010TA 7.5% 193.3 / 3.6 66intergenic (‑187/+23)pcaP/galP‑IIporin/porin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base A (3/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACT  >  NC_002947/1575054‑1575345
                                                                                                                                                   |                                                                                                                                                
gATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTa                                                                                                                                                 >  1:165733/1‑149 (MQ=255)
   aGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATcc                                                                                                                                              >  1:108404/1‑149 (MQ=255)
   aGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATcc                                                                                                                                              >  5:239146/1‑149 (MQ=255)
       aTCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATAcgcg                                                                                                                                           >  5:36185/1‑148 (MQ=255)
           ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAagag                                                                                                                                      >  3:85107/1‑149 (MQ=255)
            gATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAgg                                                                                                                                     >  1:146889/1‑149 (MQ=255)
                  cTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAACTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               <  5:200402/149‑1 (MQ=255)
                     cAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAg                                                                                                                            >  3:347245/1‑149 (MQ=255)
                          accaccATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTcac                                                                                                                        <  2:165733/148‑1 (MQ=255)
                             accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               >  8:4776/1‑138 (MQ=255)
                             accaTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               <  7:4776/138‑1 (MQ=255)
                               catcatCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGTGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               <  7:147356/136‑1 (MQ=255)
                               catcatCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGTGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               >  8:147356/1‑136 (MQ=255)
                               catcatCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACagaaga                                                                                                                  <  4:347245/149‑1 (MQ=255)
                                 tcatcaGCTCAGCGAATTGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCTGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGAtt                                                                                                                <  1:187796/149‑1 (MQ=255)
                                 tcatcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               <  5:55367/134‑1 (MQ=255)
                                 tcatcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               >  6:55367/1‑134 (MQ=255)
                                   atcaGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTCCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCa                                                                                                              <  8:189902/149‑1 (MQ=255)
                                    tcagctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                             <  2:146889/149‑1 (MQ=255)
                                    tcagctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                             >  3:344505/1‑149 (MQ=255)
                                         tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               <  6:328630/126‑1 (MQ=255)
                                         tcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               >  5:328630/1‑126 (MQ=255)
                                          cagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAgg                                                                                                                                     <  7:261640/119‑1 (MQ=38)
                                          cagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAgg                                                                                                                                     >  8:261640/1‑119 (MQ=38)
                                               aaTGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTca                                                                                                  <  7:39202/149‑1 (MQ=255)
                                                   gcggcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACaa                                                                                               <  4:85107/148‑1 (MQ=255)
                                                          cTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCgg                                                                                       >  3:220195/1‑149 (MQ=255)
                                                              cACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtct                                                                                   <  2:108404/149‑1 (MQ=255)
                                                                    gCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               <  5:102896/99‑1 (MQ=255)
                                                                    gCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                               >  6:102896/1‑99 (MQ=255)
                                                                     cAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGaa                                                                            >  3:297767/1‑149 (MQ=255)
                                                                           aTGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGt                                                                       <  6:322447/148‑1 (MQ=255)
                                                                           aTGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGt                                                                       <  3:237737/148‑1 (MQ=255)
                                                                               cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTg                                                                  <  4:344505/149‑1 (MQ=255)
                                                                               cAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTg                                                                  >  8:325167/1‑149 (MQ=255)
                                                                                  ggTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCt                                                                >  2:206071/1‑148 (MQ=255)
                                                                                    ttttCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTg                                                               >  6:300560/1‑147 (MQ=255)
                                                                                         atcaCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGAGCTGAAGTcgc                                                        >  6:41861/1‑149 (MQ=255)
                                                                                           caCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGc                                                                                                            <  5:102984/95‑1 (MQ=255)
                                                                                           caCGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGc                                                                                                            >  6:102984/1‑95 (MQ=255)
                                                                                              gCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGc                                                   <  4:297767/149‑1 (MQ=255)
                                                                                                     ccAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCCTGGTc                                             <  7:325167/148‑1 (MQ=255)
                                                                                                           atgCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGAGCTGAAGTCGCGACGCATGGTCGAGTTg                                       <  5:41861/148‑1 (MQ=255)
                                                                                                               cTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGt                                                                       <  4:218467/112‑1 (MQ=255)
                                                                                                               cTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGt                                                                       >  3:218467/1‑112 (MQ=255)
                                                                                                               cTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCa                                  >  1:173832/1‑149 (MQ=255)
                                                                                                                ttCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAg                                                            <  8:281663/122‑1 (MQ=255)
                                                                                                                ttCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                 >  4:207911/1‑149 (MQ=255)
                                                                                                                 tCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                      <  3:109312/143‑1 (MQ=255)
                                                                                                                 tCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                      >  4:109312/1‑143 (MQ=255)
                                                                                                                   aaaaGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAttt                               >  5:100707/1‑148 (MQ=255)
                                                                                                                   aaaaGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAttt                               >  3:17410/1‑148 (MQ=255)
                                                                                                                     aaccgagggCGTCTGGCCTAATCGCGGCTTAAGACGCGAAGAGGCCAATTGACTCACACAAGATTCAGCCGATAACTCACAATCCCGCGGCTCTCATCGCACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAg                            <  4:220195/145‑1 (MQ=255)
                                                                                                                      aggtagggCGTCTGGCCTATTCGCTGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGc                           <  6:36185/149‑1 (MQ=255)
                                                                                                                          agggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGa                         >  2:347415/1‑147 (MQ=255)
                                                                                                                               gTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTcc                  <  1:206071/149‑1 (MQ=255)
                                                                                                                                    gCCTCTTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTg               >  2:183688/1‑147 (MQ=255)
                                                                                                                                     ccTCTTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAg            >  3:168794/1‑149 (MQ=255)
                                                                                                                                     ccTATTCGCGTGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAg            <  6:239146/149‑1 (MQ=255)
                                                                                                                                      cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAg                            <  6:154301/132‑1 (MQ=255)
                                                                                                                                      cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAg                            >  5:154301/1‑132 (MQ=255)
                                                                                                                                       tATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCt          >  3:70905/1‑149 (MQ=255)
                                                                                                                                            gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc      >  4:99607/1‑148 (MQ=255)
                                                                                                                                            gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc      <  5:300560/148‑1 (MQ=255)
                                                                                                                                             cgtgTTTATCAGCGAAGAGGCCAATAGAGTCACAGAAGATTCAGCGGATACCTCACAATCAAGCGGATCTAATCGAACGCGTTGGTGCTGAAGTCGCGACGCCTGGTCGAGTTGCGCCATTTCAGCCACAGGTCCTTGAACCTGCCCg     >  2:281085/4‑148 (MQ=255)
                                                                                                                                               ggTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACt  <  2:173832/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGACT  >  NC_002947/1575054‑1575345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑