Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 85,096 C→T 13.5% intergenic (+74/+56) aroE → / ← PP_0075 shikimate 5‑dehydrogenase/choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294785,0960CT13.5% 92.8 / 5.3 37intergenic (+74/+56)aroE/PP_0075shikimate 5‑dehydrogenase/choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base C (16/16);  new base T (3/2);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01

TATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTCAAAC  >  NC_002947/84950‑85243
                                                                                                                                                   |                                                                                                                                                   
tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctc                                                                                                                                                     <  5:184516/147‑1 (MQ=255)
tATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCctc                                                                                                                                                     <  4:160081/147‑1 (MQ=255)
      gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg                                                                                                                                                 <  7:282581/145‑1 (MQ=255)
      gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg                                                                                                                                                 <  5:214303/145‑1 (MQ=255)
      gcggggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg                                                                                                                                                 <  5:219522/145‑1 (MQ=255)
           gcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCCCTTTGCGGCCCAATCGCGACGCAAGGCCGTTACAACAAAGTA‑CCTCGTACGCCGCTCCTCATTGGGAgccgcc                                                                                                                                        >  8:144556/1‑149 (MQ=255)
             gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCtt                                                                                                                                      <  7:144556/149‑1 (MQ=255)
             gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCtt                                                                                                                                      <  5:8884/149‑1 (MQ=21)
                           ggCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGCCGCAAGGCCGTTCCCACACAGTA‑CCTCCTACGCCGGGGCTCAGTGGGAGCGGGCGTGGGGCGCGATGGGc                                                                                                                        >  2:189188/1‑149 (MQ=255)
                             cgcCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTg                                                                                                                      <  6:342210/149‑1 (MQ=255)
                                     tgggcgcaaCGGCGCCGGCAACTCGCTCCGCGCTGAGCGGGCCGGGGCCGCTTGGCGGCCCGACCGCGACGCCAGGCCGTGCCCACAAAGTA‑CCTCGTACGCCGCTCCTCAGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCa                                                                                                               <  5:184312/141‑1 (MQ=255)
                                                   ccGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGATCCCCCAAAGTA‑CGTCGTACCCCGATCCTCAGTGGGAGCGGCCTTGCCTCGCGATGGGCTGCAAAGCAGAACCAGAAACCCaa                                                                                                >  6:103771/1‑149 (MQ=39)
                                                          ctcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAAt                                                                                                     >  5:157166/1‑137 (MQ=255)
                                                          ctcgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGGCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAAt                                                                                                     <  6:157166/137‑1 (MQ=255)
                                                                         gCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc                                                                                <  8:275379/143‑1 (MQ=255)
                                                                         gCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAcc                                                                                >  7:275379/1‑143 (MQ=255)
                                                                             gatagggCCGCCAAGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGca                                                                      >  5:330392/5‑149 (MQ=255)
                                                                                      gCTTGGCGGCCATATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGCATCCCAACCCCATTCCGCAAACCGGATCGGGCACAACTcccc                                                             >  4:256172/1‑147 (MQ=255)
                                                                                                cccaTCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc                                                   >  2:104283/4‑149 (MQ=17)
                                                                                                ccTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGc                                                   >  3:296073/1‑149 (MQ=255)
                                                                                                 ccaTCGCGACGCAAGGCCGCTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCa                                                     <  4:78503/144‑1 (MQ=255)
                                                                                                 ccaTCGCGACGCAAGGCCGCTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCa                                                     >  3:78503/3‑146 (MQ=255)
                                                                                                      gcgACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTg                                              >  6:317638/1‑148 (MQ=255)
                                                                                                       cgACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc                                             >  5:322090/1‑148 (MQ=255)
                                                                                                             aaGGCCGTTCCCACAATGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcct                                      <  5:103771/149‑1 (MQ=255)
                                                                                                               ggCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATACCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCg                                    <  2:239445/149‑1 (MQ=255)
                                                                                                                gCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcctc                                     >  2:77986/1‑147 (MQ=255)
                                                                                                                gCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACGGCCTTGCGTCGCGATAGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGa                                   >  2:97975/1‑149 (MQ=21)
                                                                                                                    ctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATc                                                                           >  8:70928/2‑105 (MQ=17)
                                                                                                                    ctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATc                                                                           <  7:70928/104‑1 (MQ=17)
                                                                                                                       ccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTg                            >  4:129832/1‑149 (MQ=255)
                                                                                                                           aaaGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTc                                                                                     <  6:23290/88‑1 (MQ=255)
                                                                                                                           aaaGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTc                                                                                     >  5:23290/1‑88 (MQ=255)
                                                                                                                              gTA‑CGTCGTACGCCTATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCCCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTg                     <  3:256172/149‑1 (MQ=255)
                                                                                                                                      gtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGt              <  6:322090/149‑1 (MQ=255)
                                                                                                                                            cGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACCACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCggagagcg         >  1:62363/1‑142 (MQ=255)
                                                                                                                                            cGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCCCCTGCGGCCTGGCCCGGTGCAAgg        >  7:248735/1‑149 (MQ=255)
                                                                                                                                            cGAGCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGGCTGGCCCg                <  2:62363/141‑1 (MQ=255)
                                                                                                                                              aTCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTc      <  5:317638/149‑1 (MQ=255)
                                                                                                                                                 ctctGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTCaaa   <  1:130721/149‑1 (MQ=255)
                                                                                                                                                  tctGTGGGAGCGGCCTTGTGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTCAAAc  >  7:91081/1‑149 (MQ=255)
                                                                                                                                                  tctGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTCaaa   >  3:300000/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTCAAAC  >  NC_002947/84950‑85243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑