Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,598,834 C→G 7.4% intergenic (‑73/+58) dctB ← / ← bglX C4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,598,8340CG 7.4% 167.1 / 3.4 54intergenic (‑73/+58)dctB/bglXC4‑dicarboxylate transport sensor protein/beta‑D‑glucoside glucohydrolase
Reads supporting (aligned to +/- strand):  ref base C (27/23);  new base G (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01

AGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCTGC  >  NC_002947/1598694‑1598979
                                                                                                                                            |                                                                                                                                                 
aGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGTGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCt                                                                                                                                            <  2:215301/148‑1 (MQ=255)
   gCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTa                                                                                                                                         <  1:117007/148‑1 (MQ=255)
           cAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGtt                                                                                                                                >  7:217919/1‑149 (MQ=255)
             gCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGttt                                                                                                                               >  2:236524/1‑148 (MQ=255)
                   tCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCcgc                                                                                                                        >  8:220457/1‑149 (MQ=255)
                   tCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCcgc                                                                                                                        >  7:16532/1‑149 (MQ=255)
                   tCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCcgc                                                                                                                        >  2:221886/1‑149 (MQ=255)
                       ctgggTGTTCAGCCGCAGACGCAGGGCGGGTATCGAATAGGCGTTCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTGCTGCAGAGTACCTGTAg                                                                                                                                        <  3:125490/127‑1 (MQ=16)
                                 aGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGaa                                                                                                           >  6:309758/1‑148 (MQ=255)
                                   ccccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAg                                                                                                        >  7:96172/1‑149 (MQ=255)
                                     ccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggc                                                                                                      >  7:330092/1‑149 (MQ=255)
                                     ccAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAggc                                                                                                      >  1:32297/1‑149 (MQ=255)
                                         aCGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCCCGAAAAGGCCGGAACAGGCGGcc                                                                                                  >  6:224701/1‑149 (MQ=255)
                                            cAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCttt                                                                                               <  1:236524/149‑1 (MQ=255)
                                                       aGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAg                                                                                    <  5:309758/149‑1 (MQ=255)
                                                         gAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGc                                                                                   >  2:243932/1‑148 (MQ=255)
                                                                  aTCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCAGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAg                                                                          >  7:225627/1‑148 (MQ=255)
                                                                      ggggCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCa                                                                             <  3:305098/141‑1 (MQ=255)
                                                                      ggggCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCa                                                                             >  4:305098/1‑141 (MQ=255)
                                                                           ggATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTctgctgct                                                                <  8:96172/149‑1 (MQ=255)
                                                                                    ggtggGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCt                                                       <  2:32297/149‑1 (MQ=255)
                                                                                      tggGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTgg                                                     <  6:123366/149‑1 (MQ=255)
                                                                                           tGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATc                                                <  8:217919/149‑1 (MQ=255)
                                                                                           tGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATc                                                <  7:220457/149‑1 (MQ=255)
                                                                                            gTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATc                                                >  4:76319/1‑148 (MQ=255)
                                                                                             tCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCa                                              <  8:16532/149‑1 (MQ=255)
                                                                                                tctgctgcctATTGTCGGGTATACCCGCGAGTAATGGTAGTCTGCTGCTCCTGTAGGCGCGTTTTTACCCTCGAAAAGGCCTTAAGAGGCGGCCTTTTTCAGTCAATGCTATTCATTTCTATGCTGCTGGACCTGGTTGGAATCCatg                                            <  4:59415/144‑3 (MQ=255)
                                                                                                 ttccggcctTTTCGCGGGTAAAACCGCGCCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTACCCGCGAAGAGGCAGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGccc                                          >  5:60433/6‑149 (MQ=17)
                                                                                                    ctgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAACGCTCTGCTGCTGCACCGCCTGGGAATCCAGccagat                                       >  3:59415/1‑145 (MQ=255)
                                                                                                    ctgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTgg                                                     >  8:251913/1‑135 (MQ=255)
                                                                                                    ctgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTgg                                                     <  7:251913/135‑1 (MQ=255)
                                                                                                    ctgcctCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTAc                                       <  6:73666/149‑1 (MQ=255)
                                                                                                            tCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCGGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCGTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACAt                               >  2:337237/1‑149 (MQ=255)
                                                                                                              gcgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGa                                                   >  2:203117/1‑127 (MQ=255)
                                                                                                              gcgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACAtt                              >  3:188321/1‑148 (MQ=255)
                                                                                                              gcgGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATACAAAGCTCTGCTGCTGCACCGCCTGGGa                                                   <  1:203117/127‑1 (MQ=255)
                                                                                                                gggTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGaa                           <  5:224701/149‑1 (MQ=255)
                                                                                                                  gTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGaa                                                                                                           >  3:56287/1‑67 (MQ=255)
                                                                                                                  gTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGaa                                                                                                           <  4:56287/67‑1 (MQ=255)
                                                                                                                   tAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTc                        <  1:221886/149‑1 (MQ=255)
                                                                                                                     aaCCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTcc                       <  8:330092/148‑1 (MQ=255)
                                                                                                                      aCCCGCGCCTACAGGTACTCTGCAGGACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGc                                            >  5:52671/1‑126 (MQ=255)
                                                                                                                      aCCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGc                                            <  6:52671/126‑1 (MQ=255)
                                                                                                                        ccGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCAGGACATTGAACTCCCCTg                   >  1:186385/1‑149 (MQ=255)
                                                                                                                          gcgcCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACAtt                              <  7:313851/136‑1 (MQ=25)
                                                                                                                          gcgcCTACAGGTGCTGCAGAGTACCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACAtt                              >  8:313851/1‑136 (MQ=25)
                                                                                                                              cTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGATGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCACCTGGTTCg              >  3:42716/1‑148 (MQ=255)
                                                                                                                                 cAGGTTCTCTGCAGCACCTGTAGTCGCGGTGTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCaa          <  4:42716/149‑1 (MQ=255)
                                                                                                                                   ggTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAAcc        >  8:260720/1‑149 (MQ=255)
                                                                                                                                    gTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAAccc       >  2:118041/1‑149 (MQ=255)
                                                                                                                                        tctGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCTg   >  4:325230/1‑149 (MQ=255)
                                                                                                                                         ctGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCTGc  <  8:225627/149‑1 (MQ=255)
                                                                                                                                          tGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCGTACCTGGACATTGAACTCCCCTGGtt                >  5:308595/1‑134 (MQ=255)
                                                                                                                                          tGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGtt                <  6:308595/134‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
AGTGCGGTACCCAGCACGATCAGCAGGGTGATCAGCCCCAGACGCAGGGCGCGAAAGGAAAAGGGCATCGGGGGCGGATCCGGTGGTGGGATGTCGGGGCCTGCCTCTTCGCGGGTAAACCCGCGCCTACAGGTACTCTGCAGCACCTGTAGGCGCGGTTTTACCCGCGAAAAGGCCGGAACAGGCGGCCTTTTTCAGTCAAAGCAATTCAAAGCTCTGCTGCTGCACCGCCTGGGAATCCAGCCCTACCTGGACATTGAACTCCCCTGGTTCGGCAACCCGCTGC  >  NC_002947/1598694‑1598979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑