Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	96,132	C→G	7.1%	G185R (GGC→CGC)	PP_0088 ←	alkanal monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	96,132	0	C	G	7.1%	211.2 / 5.9	70	G185R (GGC→CGC)	PP_0088	alkanal monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (35/30);  new base G (2/3);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

TCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGAA  >  NC_002947/95991‑96279                                                                                                                                              |                                                                                                                                                    tCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATg                                                                                                                                              >  5:282492/1‑149 (MQ=255)  cTGCGCCACCACCATGGGATTGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGc                                                                                                                                             >  8:339272/1‑149 (MQ=255)   tGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGTCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGcc                                                                                                                                            >  6:233445/1‑149 (MQ=255)    gcgcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGccc                                                                                                                                           >  8:108174/1‑149 (MQ=255)    gcgcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGccc                                                                                                                                           >  8:48988/1‑149 (MQ=255)    gcgcCACCACCATCGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCTAGGGCTTGAAAAGGATGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGCCTCGAAATGCGAGTCGAAGGCGTACGGCATGCCGCGCATGccc                                                                                                                                           >  5:252348/1‑149 (MQ=255)     cgcCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCGCGGCATGcc                                                                                                                                            <  8:5205/147‑1 (MQ=255)       ccaccaccaTGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGc                                                                                                                                         >  6:89488/1‑148 (MQ=255)       ccaccaccaTGGGAAAGCCCAGCATCACTTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCGCGGCATGccctac                                                                                                                                        <  1:287374/149‑4 (MQ=255)              caTGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTggg                                                                                                                                 >  8:123/1‑149 (MQ=255)                tGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCa                                                                                                                               <  2:244331/149‑1 (MQ=255)                       gCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGaa                                                                                                                          >  4:6864/1‑147 (MQ=255)                         ccAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGACCAgg                                                                                                                      >  6:98135/1‑149 (MQ=255)                         ccAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAgg                                                                                                                      >  7:92471/1‑149 (MQ=255)                          cAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGc                                                                                                                     <  7:339272/149‑1 (MQ=255)                           aGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCt                                                                                                                    >  7:172305/1‑149 (MQ=255)                            gCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTg                                                                                                                   <  7:48988/149‑1 (MQ=255)                            gCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTg                                                                                                                   >  6:230376/1‑149 (MQ=255)                            gCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTg                                                                                                                   <  7:108174/149‑1 (MQ=255)                                 acaTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATcc                                                                                                              <  5:89488/149‑1 (MQ=255)                                 acaTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATcc                                                                                                              >  4:250427/1‑149 (MQ=255)                                   aTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAg                                                                                                                       >  2:222214/1‑138 (MQ=255)                                   aTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAg                                                                                                                       <  1:222214/138‑1 (MQ=255)                                   aTACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCa                                                                                                            <  2:175397/149‑1 (MQ=255)                                   aTACGGCTTGTCCAGCGTGGTCCAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCCGCTGGGCACTGAACAGGCTGGATcc                                                                                                              >  8:67746/1‑147 (MQ=255)                                     aCGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAg                                                                                                           >  1:198510/1‑148 (MQ=255)                                            gTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACcaca                                                                                                   >  2:287186/1‑149 (MQ=255)                                            gTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACcac                                                                                                    <  1:353696/148‑1 (MQ=255)                                             tCCAGCGTGGTCGAGCGCTTGAAATCGTTGCGGTACACGCGAATGGCCTCGAGCATGTAGCGTGGAGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGCATCCCAGCAACCACAt                                                                                                  >  3:47035/1‑149 (MQ=255)                                               cAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGTCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATc                                                                                                 <  5:233445/148‑1 (MQ=255)                                               cAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATc                                                                                                 <  8:92471/148‑1 (MQ=255)                                               cAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCg                                                                                                >  7:46033/1‑149 (MQ=255)                                               cAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCg                                                                                                <  1:166004/149‑1 (MQ=255)                                                 gCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGc                                                                                              <  6:282492/149‑1 (MQ=255)                                                       tCGATGGCTTGTTATGGTTGCGGTACAGGCGAATGGCCTCGTGCATGTTGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCg                                                                                        <  4:47035/149‑1 (MQ=255)                                                         gAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGgt                                                                                      <  3:250427/149‑1 (MQ=255)                                                           gggCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGgtgt                                                                                    <  7:123/149‑1 (MQ=255)                                                            ggCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTc                                                                                   <  1:287186/149‑1 (MQ=255)                                                               ttGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTa                                                                                >  2:335781/1‑149 (MQ=255)                                                                  aaaTGGTTCCCGTACACCCGAATCGCCTCGAGCATCTAGCGTGGAGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCACCTGGGCACTCAACAGGCTCGATCCCAGCAACCACATCTCCACCTCGGTGTCGTAccc                                                                             >  6:272138/1‑149 (MQ=255)                                                                         tGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACg                                                                      >  5:100834/1‑149 (MQ=255)                                                                            ggTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGCCGTACCGGGGTACgaca                                                                   <  2:198510/149‑4 (MQ=255)                                                                             gTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACgg                                                                     <  6:69714/146‑1 (MQ=255)                                                                             gTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACgg                                                                     >  5:69714/1‑146 (MQ=255)                                                                                 acGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCAc                                                              >  1:156479/1‑149 (MQ=255)                                                                                 acGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCAc                                                              >  6:12725/1‑149 (MQ=255)                                                                                   gcgAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACtt                                                            <  5:12725/149‑1 (MQ=255)                                                                                   gcgAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACtt                                                            <  3:6864/149‑1 (MQ=255)                                                                                     gAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACca                                                                                                     <  4:302275/106‑1 (MQ=255)                                                                                     gAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACca                                                                                                     >  3:302275/1‑106 (MQ=255)                                                                                        tGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGa                                                       <  8:172305/149‑1 (MQ=255)                                                                                          gCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGat                                                      <  8:46033/148‑1 (MQ=255)                                                                                            cTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGc                                                                                                                                         >  1:47273/1‑63 (MQ=255)                                                                                            cTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGc                                                                                                                                         <  2:47273/63‑1 (MQ=255)                                                                                                tGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGt                                               >  7:81874/1‑149 (MQ=255)                                                                                                 gCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTg                                              >  8:300813/1‑149 (MQ=255)                                                                                                     gTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTg                                          >  3:3529/1‑149 (MQ=255)                                                                                                      tAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTg                                          <  2:156479/148‑1 (MQ=255)                                                                                                          gTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGAGTGGccc                                      >  3:259142/1‑148 (MQ=255)                                                                                                               gcgAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAgg                                  >  7:3329/1‑147 (MQ=255)                                                                                                                              aaGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGa                                                                  <  1:275967/100‑1 (MQ=255)                                                                                                                              aaGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGa                                                                  >  2:275967/1‑100 (MQ=255)                                                                                                                               aGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCg                <  7:300813/149‑1 (MQ=255)                                                                                                                                 ctggcgggCATGCGCGGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGaa                         >  2:154398/6‑138 (MQ=255)                                                                                                                                 ctggcgggCATGCGCGGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGaa                         <  1:154398/133‑1 (MQ=255)                                                                                                                                   gTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTCTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACa             >  4:227362/1‑148 (MQ=255)                                                                                                                                    gcgggCATGCGCGGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATc           >  8:49610/3‑149 (MQ=255)                                                                                                                                       ggCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTc        <  3:215095/149‑1 (MQ=255)                                                                                                                                         cATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGAGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTg      <  2:228378/149‑1 (MQ=255)                                                                                                                                          aTGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTgg     <  8:81874/149‑1 (MQ=255)                                                                                                                                             ccGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGaa  <  1:335781/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    TCTGCGCCACCACCATGGGAATGCCCAGCATCACATACGGCTTGTCCAGCGTGGTCGAGGGCTTGAAATGGTTGCGGTACACGCGAATGGCCTCGTGCATGTAGCGTGGCGCGAAATGCGAGGCGAAGGCGTAGGGCATGCCGCGCATGCCCGCCAGCTGGGCACTGAACAGGCTGGATCCCAGCAACCACATCGGCACGTCGGTGTCGTACCCCGGTACGGCGATCACTTTTTGATCATCAGTGCGTGGCCCGAGGTAGCGCGAAAGCTCCTCGACATCGTCTGGGAA  >  NC_002947/95991‑96279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑