Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,648,957 T→A 13.3% intergenic (‑236/+60) oprB‑II ← / ← PP_1446 carbohydrate‑selective porin/TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,648,9570TA13.3% 136.5 / 8.1 51intergenic (‑236/+60)oprB‑II/PP_1446carbohydrate‑selective porin/TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (25/19);  new base A (2/5);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGT  >  NC_002947/1648811‑1649103
                                                                                                                                                  |                                                                                                                                                  
tGAATCCTTTCAGCTTGCGGCCGGGAGGTTATAGCGCTAGGTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGCCAGGGGTTGGAGCGGGTTCACCCGCGAATGCGCCGGTTGc                                                                                                                                                  <  4:125063/149‑1 (MQ=255)
          cAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATc                                                                                                                                         <  3:144325/148‑1 (MQ=255)
                   gcggGGAGGATATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATACGATGGTAGGGCAACCGGCGCATTCGCgg                                                                                                                               <  3:216997/149‑1 (MQ=25)
                   gcggGGAGGATATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATACGATGGTAGGGCAACCGGCGCATTCGCgg                                                                                                                               <  2:283003/149‑1 (MQ=25)
                   gcggGGAGGATATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAAGAGCGGGTTCACCCGCGAATACGATGGTAGGGCAACCGGCGCATTCGCgg                                                                                                                               <  1:268999/149‑1 (MQ=25)
                           gaTATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAAccc                                                                                                                       >  2:152113/1‑149 (MQ=255)
                              ataGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAAc                                                                                                                         <  5:70766/144‑1 (MQ=255)
                              ataGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAAc                                                                                                                         >  6:70766/1‑144 (MQ=255)
                                  gggAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTAtt                                                                                                                                    >  4:14050/1‑129 (MQ=255)
                                  gggAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTAtt                                                                                                                                    <  3:14050/129‑1 (MQ=255)
                                    gAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTaca                                                                                                              >  3:297773/1‑149 (MQ=255)
                                    gAAGTCATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTaca                                                                                                              <  1:91818/149‑1 (MQ=255)
                                          ataAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGAt                                                                                                         <  4:297773/148‑1 (MQ=255)
                                              gAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtcg                                                                                                    >  6:93115/1‑149 (MQ=255)
                                               aaaaaaGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtcgt                                                                                                   >  4:156507/1‑149 (MQ=255)
                                                 aaaaGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtcgtcg                                                                                                 >  6:255818/1‑149 (MQ=255)
                                                    aGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCtt                                                                                              >  7:185265/1‑149 (MQ=255)
                                                      aCAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATACGATGGTAGGGCAACCGGCGCATTCGCGGGTGAACCCGCTc                                                                                                                   >  8:102801/1‑126 (MQ=17)
                                                      aCAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATACGATGGTAGGGCAACCGGCGCATTCGCGGGTGAACCCGCTc                                                                                                                   <  7:102801/126‑1 (MQ=17)
                                                        aaaTTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCAACGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTgg                                                                                          >  6:138023/1‑149 (MQ=255)
                                                        aaaTTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCAACCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTgg                                                                                          >  7:336066/1‑149 (MQ=255)
                                                             gTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCa                                                                                      <  8:185265/148‑1 (MQ=255)
                                                                 cAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCtt                                                                                              <  5:148380/136‑1 (MQ=255)
                                                                 cAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCtt                                                                                              >  6:148380/1‑136 (MQ=255)
                                                                      gATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAggtg                                                                               >  7:150915/1‑146 (MQ=255)
                                                                      gATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAggtg                                                                               <  8:150915/146‑1 (MQ=255)
                                                                           ttgttgAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACAcc                                                                                                            <  2:101156/112‑1 (MQ=255)
                                                                           ttgttgAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACAcc                                                                                                            >  1:101156/1‑112 (MQ=255)
                                                                                         aCAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGc                                                         <  3:156507/149‑1 (MQ=255)
                                                                                           aGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTa                                                       >  5:282046/1‑149 (MQ=255)
                                                                                             gcgtcTTTAATCGAGGCAGGTGTAGGAGCGGGTTCACCAGCGAATGCGGCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTAc                                                      <  8:336066/147‑1 (MQ=255)
                                                                                               gtcATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGcc                                                   >  4:147468/1‑149 (MQ=255)
                                                                                                tcATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTcc                                                                                                                  <  4:35532/85‑1 (MQ=255)
                                                                                                tcATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTcc                                                                                                                  >  3:35532/1‑85 (MQ=255)
                                                                                                   ttAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCgg                                               >  3:113417/1‑149 (MQ=255)
                                                                                                      aTCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCAACGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGGGGTACGCAACCCGACTTCCACGCTACGCCCCGgcgc                                            <  5:138023/149‑1 (MQ=255)
                                                                                                             cAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCg                                     <  1:152113/149‑1 (MQ=255)
                                                                                                              aGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGa                                    <  3:147468/149‑1 (MQ=255)
                                                                                                                gtgtAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGata                                  >  5:177182/1‑149 (MQ=255)
                                                                                                                      gaacGGGTTCACCCGCGAATACGATGGTAGGGCAACCGGCGCATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAGCCCGACATCCACGCTACGCCCCGGCGCCGGTGCGATATcgcg                             >  4:93344/4‑148 (MQ=18)
                                                                                                                      gAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCgcgc                            <  6:282046/149‑1 (MQ=255)
                                                                                                                       aGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCa                           <  5:255818/149‑1 (MQ=255)
                                                                                                                        gCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAg                          >  4:104532/1‑149 (MQ=255)
                                                                                                                         cGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACt                                                                <  1:101452/110‑1 (MQ=255)
                                                                                                                         cGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACt                                                                >  2:101452/1‑110 (MQ=255)
                                                                                                                            gTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATc                      >  5:38662/1‑149 (MQ=255)
                                                                                                                            gTTCACCCGCGAATACGATGGTAGGGCATCCGGCGCATTCGCGGGTGAACCCGTTCCTACTCCGATCTTCGTCGCTTGTGGTTCAGAAGGGGGTAGGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATc                      <  3:93344/149‑1 (MQ=21)
                                                                                                                              caaCCGGCGCATTCGCGGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCg                     >  5:277243/3‑148 (MQ=255)
                                                                                                                                            gcCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCa      >  4:236072/1‑149 (MQ=255)
                                                                                                                                             ccGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCAc     >  7:138964/1‑149 (MQ=255)
                                                                                                                                                gTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGTCATAGCGCACGGt  >  6:365907/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATCGAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGT  >  NC_002947/1648811‑1649103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑