Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,671,167 G→T 7.5% V574V (GTC→GTA)  PP_1467 ← NhaP‑type Na+(K+)/H+ antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,671,1670GT 7.5% 214.2 / 6.5 67V574V (GTC→GTA) PP_1467NhaP‑type Na+(K+)/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base G (30/32);  new base T (3/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01

AGCTTCGTCGCCATCGACGAGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACGGTTGGCCAGTTGCAGA  >  NC_002947/1671023‑1671308
                                                                                                                                                |                                                                                                                                             
aGCTTCGTCGCCATCGACGAGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGAccc                                                                                                                                           >  7:61401/1‑149 (MQ=255)
       tcgCCATCGACGAGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTg                                                                                                                                    >  5:337833/1‑149 (MQ=255)
        cgatggcgacgaGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGccc                                                                                                                                      >  6:83194/7‑146 (MQ=255)
        cgatggcgacgaGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGccc                                                                                                                                      <  5:83194/140‑1 (MQ=255)
         gCCATCGACGAGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGc                                                                                                                                  >  6:56651/1‑149 (MQ=255)
            aTCGACGAGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAAGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCAt                                                                                                                               >  8:256288/1‑149 (MQ=255)
                acgaGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATcgcg                                                                                                                           <  6:162741/149‑1 (MQ=255)
                  gaGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCggg                                                                                                                         >  2:193096/1‑149 (MQ=255)
                  gaGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGAGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCggg                                                                                                                         >  8:149241/1‑149 (MQ=255)
                   aGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGc                                                                                                                        <  5:56651/149‑1 (MQ=255)
                     gACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTTGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCGGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCg                                                                                                                       >  6:369531/1‑148 (MQ=255)
                       cAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGCTCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGaa                                                                                                                     >  7:40355/1‑148 (MQ=255)
                       cAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAAc                                                                                                                    <  5:127198/149‑1 (MQ=255)
                        aaGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGGTCCATGTagccagcc                                                                                                                                       <  2:1689/129‑1 (MQ=255)
                        aaGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGGTCCATGTagccagcc                                                                                                                                       >  1:1689/1‑129 (MQ=255)
                        aaGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACa                                                                                                                   >  4:57556/1‑149 (MQ=255)
                              ccGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAgt                                                                                                             <  1:42769/149‑1 (MQ=255)
                               cGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCgg                                                                                                                          >  2:201971/1‑135 (MQ=255)
                               cGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCgg                                                                                                                          <  1:201971/135‑1 (MQ=255)
                                           tgcCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGc                                                                                                 >  4:52460/1‑148 (MQ=255)
                                           tgcCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGc                                                                                                 >  4:52462/1‑148 (MQ=255)
                                             ccAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGCTCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGcc                                                                                                <  8:40355/147‑1 (MQ=255)
                                             ccAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGcc                                                                                                <  3:309013/147‑1 (MQ=255)
                                                ggCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATg                                                                                             >  4:56002/1‑147 (MQ=255)
                                                   gcCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTg                                                                                        >  1:279192/1‑149 (MQ=255)
                                                      tGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCt                                                                                     >  5:214653/1‑149 (MQ=255)
                                                        aGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTgg                                                                                   <  4:164283/149‑1 (MQ=255)
                                                         gTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGt                                                                                  <  6:337833/149‑1 (MQ=255)
                                                         gTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGt                                                                                  <  3:52462/149‑1 (MQ=255)
                                                         gTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGt                                                                                  <  3:52460/149‑1 (MQ=255)
                                                         gTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGt                                                                                  <  2:279192/149‑1 (MQ=255)
                                                          tATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTa                                                                                 <  3:57556/149‑1 (MQ=255)
                                                          tATCACGGCTCCGGTGCAGGCTCGATCAAAGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTa                                                                                 <  7:256288/149‑1 (MQ=255)
                                                                      ggTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGcc                                                                     <  3:56002/149‑1 (MQ=255)
                                                                              cTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGAGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCtt                                                             <  7:149241/149‑1 (MQ=255)
                                                                                    cAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGt                                                         >  5:647/1‑147 (MQ=255)
                                                                                     aaGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCa                                                       <  6:214653/148‑1 (MQ=255)
                                                                                       ggCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGAccc                                                                                                                                           >  5:85223/1‑62 (MQ=255)
                                                                                       ggCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGAccc                                                                                                                                           <  6:85223/62‑1 (MQ=255)
                                                                                       ggCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGt                                                    <  8:61401/149‑1 (MQ=255)
                                                                                             caGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTAACAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCtt                                                             >  3:201442/1‑134 (MQ=255)
                                                                                             caGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTAACAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCtt                                                             <  4:201442/134‑1 (MQ=255)
                                                                                                 gTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCtata                                          <  3:276690/149‑1 (MQ=255)
                                                                                                  tCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCtata                                          >  6:86648/1‑148 (MQ=255)
                                                                                                   ccAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATcc                                                                          <  3:150269/115‑1 (MQ=255)
                                                                                                   ccAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATcc                                                                          >  4:150269/1‑115 (MQ=255)
                                                                                                     aGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGACCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCTTGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTg                                               <  4:82159/140‑1 (MQ=255)
                                                                                                     aGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGACCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTg                                               >  3:82159/1‑140 (MQ=255)
                                                                                                      gCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCt                                     <  1:193096/149‑1 (MQ=255)
                                                                                                        ccGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCg                                   <  3:178070/149‑1 (MQ=255)
                                                                                                           gctgcggctgcAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGgcg                                >  6:183081/1‑149 (MQ=255)
                                                                                                             tgcggctgcAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGcc                              >  1:102130/1‑149 (MQ=255)
                                                                                                               cggctgcAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCt                             <  5:183081/148‑1 (MQ=255)
                                                                                                                      aaCGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATg                        >  4:62889/1‑146 (MQ=255)
                                                                                                                       aCGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGa                    >  3:174399/1‑149 (MQ=255)
                                                                                                                         gggTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCt                             >  6:258347/1‑138 (MQ=255)
                                                                                                                         gggTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCt                             <  5:258347/138‑1 (MQ=255)
                                                                                                                         gggTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACg                  <  5:86648/149‑1 (MQ=255)
                                                                                                                           gTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGTGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCg                                                           >  7:79516/1‑106 (MQ=255)
                                                                                                                           gTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGTGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCg                                                           <  8:79516/106‑1 (MQ=255)
                                                                                                                              gccAGGGCTGGGTCCATGTAGCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACGGTTgg             >  1:34802/3‑149 (MQ=255)
                                                                                                                                 aGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACGGTTGGc            >  8:822/1‑147 (MQ=255)
                                                                                                                                   ggctgggtCCATGTAGCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACg                  >  7:305562/1‑139 (MQ=255)
                                                                                                                                   ggctgggtCCATGTAGCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACg                  <  8:305562/139‑1 (MQ=255)
                                                                                                                                    gctggctACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACGGTTGGCCAGtt       <  6:647/149‑1 (MQ=255)
                                                                                                                                    gctggctACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACGGTTGGCCAGtt       <  1:204880/149‑1 (MQ=255)
                                                                                                                                          tACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACGGTTGGCCAGTTGCAGa  >  7:18729/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                             
AGCTTCGTCGCCATCGACGAGGACAAGCGCCCGGTGCCCGTGCTGCCAGGCGCCTGAGTATCACGGCTCCGGTGCAGGCTCGATCAAGGCCACCAGGGTCCAGCCCGGCTGCGGCTGCAACGGGTTATCAGGGCTGGCTACATGGACCCAGCCCTGGCCATCGCGGGCGAACAGCAAGTGTGCCCGCTCGCCATGCAGTGCCTGGTAGGCATCCCAGCCAAAGCCTTCGGTCAGGTTGGTGCTATACAGCTCGGCGCCTTGATGCAGACGGTTGGCCAGTTGCAGA  >  NC_002947/1671023‑1671308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑