Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,691,783 C→A 9.1% intergenic (+50/‑121) PP_1487 → / → PP_1488 hypothetical protein/methyl‑accepting chemotaxis transducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,691,7830CA 9.1% 167.0 / 5.5 55intergenic (+50/‑121)PP_1487/PP_1488hypothetical protein/methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base C (23/27);  new base A (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

ATGGAGCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCTTGCGCCAA  >  NC_002947/1691635‑1691927
                                                                                                                                                    |                                                                                                                                                
aTGGAGCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCgccgcc                                                                                                                                                  >  2:358305/1‑149 (MQ=255)
 tGGAGCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGccc                                                                                                                                                 <  2:67038/149‑1 (MQ=255)
     gCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTg                                                                                                                                              <  8:298195/148‑1 (MQ=255)
         gCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTgcggcg                                                                                                                                         >  7:243368/1‑149 (MQ=255)
           cTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCt                                                                                                                                       <  4:286547/149‑1 (MQ=255)
              ccTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTcc                                                                                                                                     <  1:358305/148‑1 (MQ=255)
               cTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCGTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGGCGGCGCTcgat                                                                                                                                   <  3:21263/149‑4 (MQ=39)
                     gAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTcaca                                                                                                                             <  7:29355/149‑1 (MQ=255)
                          ggCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGgcg                                                                                                                         >  4:246884/1‑148 (MQ=255)
                             ccGCCAGCTGCGGCTGGCCCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGTGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGCGATCGAGCGCCGCCCGTGAGGCGCTCCGTCTCACAGGCGCaa                                                                                                                      >  3:131483/1‑148 (MQ=255)
                              cGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCaaaa                                                                                                                    <  7:276900/149‑1 (MQ=255)
                                       cGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTgac                                                                                                            >  4:84937/1‑148 (MQ=255)
                                           tGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACaa                                                                                                       >  6:361966/1‑149 (MQ=255)
                                              aCCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATca                                                                                                    >  6:28164/1‑149 (MQ=255)
                                               cccGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATca                                                                                                    >  5:160444/1‑148 (MQ=255)
                                                ccGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTATGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTcc                                                                                                                >  8:54420/1‑135 (MQ=255)
                                                ccGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTATGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTcc                                                                                                                <  7:54420/135‑1 (MQ=255)
                                                  gggCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATc                                                                                                <  3:246884/149‑1 (MQ=255)
                                                  gggCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATc                                                                                                <  3:84937/149‑1 (MQ=255)
                                                  gggCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATc                                                                                                <  6:160444/149‑1 (MQ=255)
                                                  gggCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATc                                                                                                <  8:243368/149‑1 (MQ=255)
                                                      ttGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATa                                                                                            >  3:349336/1‑149 (MQ=255)
                                                             aGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGgcg                                                                                                                                            >  3:2464/1‑94 (MQ=38)
                                                             aGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGACGGAGCGCCGCACGGgcg                                                                                                                                            <  4:2464/94‑1 (MQ=38)
                                                                     gAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTgg                                                                             >  3:305103/1‑149 (MQ=255)
                                                                      aaaGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTggg                                                                            >  3:191822/1‑149 (MQ=255)
                                                                           cAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCtata                                                                       >  7:43665/1‑149 (MQ=255)
                                                                             ggTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACt                                                                     >  5:327526/1‑149 (MQ=255)
                                                                               tGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGc                                                                           >  7:172862/1‑141 (MQ=255)
                                                                               tGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGc                                                                           <  8:172862/141‑1 (MQ=255)
                                                                                  ggTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGc                                                                <  6:327526/149‑1 (MQ=255)
                                                                                       gCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAttt                                                           <  4:305103/149‑1 (MQ=255)
                                                                                         tGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAtttt                                                          >  6:264030/1‑148 (MQ=255)
                                                                                          gCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCta                                                                         >  8:272178/1‑132 (MQ=255)
                                                                                          gCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCta                                                                         <  7:272178/132‑1 (MQ=255)
                                                                                           cAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGAttttt                                                         <  3:272937/147‑1 (MQ=255)
                                                                                            aGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTccc                                                      <  8:43665/149‑1 (MQ=255)
                                                                                                  gTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCg                                                 <  8:13614/148‑1 (MQ=255)
                                                                                                   tGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGtt                                               <  5:264030/149‑1 (MQ=255)
                                                                                                     gCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTggg                                                                            >  2:242063/1‑118 (MQ=255)
                                                                                                     gCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTggg                                                                            <  1:242063/118‑1 (MQ=255)
                                                                                                       ggTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGa                                           <  4:172619/149‑1 (MQ=255)
                                                                                                              gCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCg                                                               <  5:129229/122‑1 (MQ=255)
                                                                                                              gCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCg                                                               >  6:129229/1‑122 (MQ=255)
                                                                                                              gCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTcc                                      <  5:28164/147‑1 (MQ=255)
                                                                                                                cACGGCCTTTTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAg                                  <  4:191822/149‑1 (MQ=255)
                                                                                                                cACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTggg                                                                            <  4:242499/107‑1 (MQ=255)
                                                                                                                cACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTggg                                                                            >  3:242499/1‑107 (MQ=255)
                                                                                                                    gCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCt                              >  2:15443/1‑149 (MQ=255)
                                                                                                                    gCCTATTGAGCGCCGGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCt                              <  4:131483/149‑1 (MQ=255)
                                                                                                                                 cTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAAc                 >  4:218213/1‑149 (MQ=255)
                                                                                                                                   gtgAGACGGAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAggg                                <  2:287436/132‑1 (MQ=38)
                                                                                                                                   gtgAGACGGAGCGCCGCACGGGCGGCGCTCGATCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAggg                                >  1:287436/1‑132 (MQ=38)
                                                                                                                                    tgAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTtgt                                             <  5:283308/118‑1 (MQ=255)
                                                                                                                                    tgAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTtgt                                             >  6:283308/1‑118 (MQ=255)
                                                                                                                                        aTCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCt          >  1:289245/1‑149 (MQ=255)
                                                                                                                                           gAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCTTgc       <  2:289245/149‑1 (MQ=255)
                                                                                                                                                ccgccCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCTTGCGCCaa  >  1:25559/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATGGAGCGTGCCTACCTGGACGAGCTGGCCCGCCAGCTGCGGCTGGACCCGGGCTTGCGCCAGGAACTGGAAAGCCAGGTGCGGTTGGCTGCAGCGTAGTGGCGGTGCTTGCCACGGCCTATTGAGCGCCTGTGAGATCGAGCGCCGCCCGTGCGGCGCTCCGTCTCACAGGCGCAAAATTCCTGACGACAATCACATCAATACCGTAAGACCTCTGGGCTATACTTCGGCGATTTTTCCCGCTCGTTGTGAATTCCTGAGGGCTGAATGTGAAGAACTGGACCTTGCGCCAA  >  NC_002947/1691635‑1691927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑