Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,748,902	A→T	5.8%	intergenic (‑235/‑97)	PP_1548 ← / → PP_5744	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,748,902	0	A	T	5.8%	218.4 / 2.8	69	intergenic (‑235/‑97)	PP_1548/PP_5744	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/26);  new base T (2/2);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

ATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGA  >  NC_002947/1748761‑1749048                                                                                                                                              |                                                                                                                                                      aTGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAATTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCa                                                                                                                                                <  5:254321/149‑1 (MQ=255)  tGTCGAGCGAGGAAGCTTGCTACGTTGCGGAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCAt                                                                                                                                               <  4:270857/149‑1 (MQ=255)      gagcgagGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTta                                                                                                                                            >  2:173518/1‑148 (MQ=255)               aGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATg                                                                                                                                  >  8:59528/1‑149 (MQ=255)                      tACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑ttt                                                                                                                          >  3:326120/1‑149 (MQ=255)                         gTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑tttttt                                                                                                                       <  4:132012/149‑1 (MQ=255)                            gCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACt                                                                                                                                                    >  5:57975/1‑118 (MQ=255)                            gCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACt                                                                                                                                                    <  6:57975/118‑1 (MQ=255)                              gTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑At                                                                                                                 >  7:274294/1‑148 (MQ=255)                              agatgagcccccaggcgccgagttaatccggcgattgagttctggtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑At                                                                                                                 >  8:319277‑M2/49‑148 (MQ=255)                               tAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGc                                                                                                               <  4:326120/149‑1 (MQ=255)                                  gagccaccaggcgccgagttaatccggcgattgagttctggtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTaa‑‑a                                                                                                                  <  6:23782‑M2/99‑1 (MQ=255)                                  gagccaccaggcgccgagttaatccggcgattgagttctggtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTaa‑‑a                                                                                                                  <  4:150121‑M2/99‑1 (MQ=255)                                  gagccaccaggcgccgagttaatccggcgattgagttctggtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTaa‑‑a                                                                                                                  >  3:150121‑M2/45‑143 (MQ=255)                                  gagccaccaggcgccgagttaatccggcgattgagttctggtggCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTaa‑‑a                                                                                                                  >  5:23782‑M2/45‑143 (MQ=255)                                    tCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCa                                                                                                          >  1:336805/1‑149 (MQ=255)                                         CAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCAttt                                                                                                                                             >  2:354718‑M1/1‑112 (MQ=255)                                         CAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCAttt                                                                                                                                             <  1:354718‑M1/112‑1 (MQ=255)                                           GAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTtgt                                                                                                   >  7:59528‑M1/1‑149 (MQ=255)                                               tCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGc                                                                                                                                 >  4:340757/1‑118 (MQ=255)                                               tCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGc                                                                                                                                 <  3:340757/118‑1 (MQ=255)                                               tCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTaa                                                                                               >  3:248708/1‑149 (MQ=255)                                               tCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTaa                                                                                               >  1:177358/1‑149 (MQ=255)                                                cgagttaatccggcgattgagttctggtggCTCATCTGCTGGTTTTTAATGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAAt                                                                                              <  7:319277‑M2/119‑1 (MQ=255)                                                cAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAAt                                                                                              >  1:146633/1‑149 (MQ=255)                                                 aaTCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAAGCATGCTTTTATAA‑‑ATGCAAGTTCGCTtgcatttg                                                                                              <  4:58751/148‑7 (MQ=21)                                                 aaTCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAAGCATGCTTTTATAA‑‑ATGCAAGTTCGCTtgcatttg                                                                                              >  3:58751/1‑142 (MQ=21)                                                    cGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑At                                                                                                                 >  3:327280/1‑126 (MQ=255)                                                    cGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑At                                                                                                                 <  4:327280/126‑1 (MQ=255)                                                     gCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTtc                                                                                          >  3:48088/1‑148 (MQ=255)                                                      ccGGATTAACTCGGTGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCaa                                                                                                             >  6:60619/1‑128 (MQ=255)                                                      ccGGATTAACTCGGTGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCaa                                                                                                             <  5:60619/128‑1 (MQ=255)                                                               cTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTAt                                                                               <  1:173518/149‑1 (MQ=255)                                                                tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAAGCATGCTTTTATAA‑‑ATGCAAGTTCGCTtgcattt                                                                                               <  3:29097/132‑6 (MQ=18)                                                                tCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGTGCTTGCATTTA‑AAAAACAAGCATGCTTTTATAA‑‑ATGCAAGTTCGCTtgcattt                                                                                               >  4:29097/1‑127 (MQ=18)                                                                     gcCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGt                                                                         >  5:67130/1‑149 (MQ=255)                                                                                cATCTGCTGGTTTTTAAAGATCTGTGGCTGCCTGAGCTGACATCTCGATTCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTa                                                               <  6:67130/148‑1 (MQ=255)                                                                                       tgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCttt                                                       >  7:289554/1‑149 (MQ=255)                                                                                       tgGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCttt                                                       >  8:270012/1‑149 (MQ=255)                                                                                          tttttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCCTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCg                                                     >  1:240366/1‑148 (MQ=255)                                                                                           ttttAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGa                                                   >  1:302822/1‑149 (MQ=255)                                                                                                aaGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCg                                              >  3:289101/1‑149 (MQ=255)                                                                                                aaGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCg                                              >  7:156236/1‑149 (MQ=255)                                                                                                    gCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgtgt                                                                  <  4:121439/125‑1 (MQ=255)                                                                                                    gCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAACGAACATGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAgtgt                                                                  >  3:121439/1‑125 (MQ=255)                                                                                                          gCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGaaa                                    <  4:48088/149‑1 (MQ=255)                                                                                                          gCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGaaa                                    <  8:274294/149‑1 (MQ=255)                                                                                                            tGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAaga                                  <  7:270012/149‑1 (MQ=255)                                                                                                             gCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAagag                                 >  2:84886/1‑149 (MQ=255)                                                                                                              ccGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAAGGGTGCAAGCACGATTGTGTAATTTTCTATTACTTTATGGATGTCAAGTGTGTAGGAGGTTTCGTATGTCGGCGCAGTaaa                                    >  1:107292/1‑147 (MQ=255)                                                                                                               cGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAagag                                 >  8:7366/1‑147 (MQ=255)                                                                                                                ggAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTATGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAg                              >  8:142504/1‑149 (MQ=255)                                                                                                                     tGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGAt                                                  >  3:223562/1‑124 (MQ=255)                                                                                                                     tGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGAt                                                  <  4:223562/124‑1 (MQ=255)                                                                                                                       ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                       >  7:56566/1‑149 (MQ=255)                                                                                                                       ccAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTAt                       >  7:61513/1‑149 (MQ=255)                                                                                                                        cAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATg                      <  8:56566/149‑1 (MQ=255)                                                                                                                        cAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATg                      <  8:61513/149‑1 (MQ=255)                                                                                                                            tCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTAGTGTATGGATGTCAAGTGTGTAGGAGCTTTCGGATCTCGGAGAAGGAAAGAGCAGTACGTATGGaa                   >  8:305678/1‑148 (MQ=255)                                                                                                                              gATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAaga                 <  8:289554/148‑1 (MQ=255)                                                                                                                               atgcaaatgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCCTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGc               <  2:240366/149‑1 (MQ=255)                                                                                                                                  caaatgcaaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTGAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTaa            >  1:321460/1‑149 (MQ=255)                                                                                                                                         aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGcc      <  2:302822/148‑1 (MQ=255)                                                                                                                                         aaGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCt     >  3:265978/1‑149 (MQ=255)                                                                                                                                          aGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCtt    <  4:248708/149‑1 (MQ=255)                                                                                                                                          aGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCtt    >  3:309517/1‑149 (MQ=255)                                                                                                                                           gCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCtt    >  3:42404/1‑148 (MQ=255)                                                                                                                                           gCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTg   >  7:325313/1‑149 (MQ=255)                                                                                                                                            cGAACTTGCATTTATATAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGATAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGa  <  2:146633/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                      ATGTCGAGCGAGGAAGCTTGCTACGTTGCGTAACGTCCACCAGAACTCAATCGCCGGATTAACTCGGCGCCTGGTGGCTCATCTGCTGGTTTTTAAAGAGCGGTGGCTGCCGGAGCTGCCAGCTCGATGCAAATGCAAGCGAACTTGCATTTATAAAAGCATGCTTG‑TTTTTTAA‑‑ATGCAAGCACGCTTGTGTAATTTTCTCTTACTGTATGGATGTCCAGTGTGTAGGAGCTTTCGGATGTCGGCGCAGGAAAGAGCAGTTCGTATGGAAGAGCTAACGAGCCTTGA  >  NC_002947/1748761‑1749048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑