Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,784,031	C→G	6.3%	A16P (GCG→CCG)	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,784,031	0	C	G	6.3%	203.3 / 3.5	63	A16P (GCG→CCG)	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (28/31);  new base G (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.64e-01

GCAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAG  >  NC_002947/1783884‑1784176                                                                                                                                                    |                                                                                                                                                  gCAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGcc                                                                                                                                                  >  3:267826/1‑149 (MQ=255)  cAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCt                                                                                                                                                 <  2:32690/149‑1 (MQ=255)   aGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTg                                                                                                                                                <  6:191607/149‑1 (MQ=255)    ggATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCt                                                                                                                                                 <  1:306892/147‑1 (MQ=255)         ttGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTg                                                                                                                                          >  6:55068/1‑149 (MQ=255)           gTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTgg                                                                                                                                         <  4:182516/148‑1 (MQ=255)           gTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGc                                                                                                                                        >  4:130277/1‑149 (MQ=255)            tCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCTCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGcc                                                                                                                                       <  8:195224/149‑1 (MQ=255)            tCGACGAGCCAGGCGCGGGCCTCGATCACCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGc                                                                                                                                        >  1:200155/1‑148 (MQ=255)               acgaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGccct                                                                                                                                     >  1:150403/1‑146 (MQ=255)                cgaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACgg                                                                                                                                   >  3:206364/1‑149 (MQ=255)                 gaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGccctcaa                                                                                                                                  <  2:150403/149‑6 (MQ=255)                        gcgcGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGACCAGTTCCGCCTGGAACTGGCCACGGTCGAACAg                                                                                                                           >  4:263749/1‑149 (MQ=255)                            gggCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCg                                                                                                                       <  4:267826/149‑1 (MQ=255)                              gCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCggg                                                                                                                     <  5:15526/149‑1 (MQ=255)                              gCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCggg                                                                                                                     <  5:244150/149‑1 (MQ=255)                               ccTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCgggg                                                                                                                    >  8:141356/1‑149 (MQ=255)                                         cGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTggg                                                                                                          <  2:191841/149‑1 (MQ=255)                                                aTGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATcgc                                                                                                   >  3:324739/1‑149 (MQ=255)                                                   tCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTc                                                                                                <  4:324739/149‑1 (MQ=255)                                                     gCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCAc                                                                                              <  7:141356/149‑1 (MQ=255)                                                           ggCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCt                                                                                        >  1:319758/1‑149 (MQ=255)                                                           ggCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCt                                                                                        <  4:206364/149‑1 (MQ=255)                                                           ggCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCt                                                                                        >  2:144129/1‑149 (MQ=255)                                                                gAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCgggg                                                                                                                    <  3:109151/116‑1 (MQ=255)                                                                gAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCgggg                                                                                                                    >  4:109151/1‑116 (MQ=255)                                                                 aaaaCGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAAGAGCTCGGGGTCCACCTGGTGCATCGCGTCACGTTCCTGTCGTg                                                                                  <  1:222187/149‑1 (MQ=255)                                                                      gTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTg                                                                             >  6:339000/1‑149 (MQ=255)                                                                       tttGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTgg                                                                            <  3:130277/149‑1 (MQ=255)                                                                         tGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGc                                                                           <  2:319758/148‑1 (MQ=255)                                                                          gTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCt                                                                         >  3:12731/1‑149 (MQ=255)                                                                             gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTc                                                                      >  6:182931/1‑149 (MQ=255)                                                                                cACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCggg                                                                   <  5:182931/149‑1 (MQ=255)                                                                                  ccTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCggg                                                                   >  7:1519/1‑147 (MQ=255)                                                                                  ccTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCggg                                                                   <  8:1519/147‑1 (MQ=255)                                                                                   cTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTg                                                                >  7:234038/1‑149 (MQ=255)                                                                                      gCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTc                                                                                     >  8:128435/1‑125 (MQ=255)                                                                                      gCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTc                                                                                     <  7:128435/125‑1 (MQ=255)                                                                                        cGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTc                                                           <  2:200155/149‑1 (MQ=255)                                                                                            cAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGc                                                                           >  7:2632/1‑129 (MQ=255)                                                                                            cAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGc                                                                           <  8:2632/129‑1 (MQ=255)                                                                                                 ggATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGATCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAg                                                  >  2:8049/1‑149 (MQ=255)                                                                                                        tttCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCgg                                           >  4:168753/1‑149 (MQ=255)                                                                                                          gcTTGAAGGCGGCGATGGGGCGCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGGCCACCTGGGGCATCGCGTCACGTTCCTGTCATGGGGTGGCCTGTCGGTGTGCGGTCAGGCCGAAGTGCGCgg                                           <  7:52011/146‑1 (MQ=255)                                                                                                            ttGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                       >  6:156394/1‑149 (MQ=255)                                                                                                            ttGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                       >  2:52590/1‑149 (MQ=255)                                                                                                              gAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCa                                                                                               >  4:153199/1‑91 (MQ=255)                                                                                                              gAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCa                                                                                               <  3:153199/91‑1 (MQ=255)                                                                                                                 ggcggcGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtc                                  <  5:55068/149‑1 (MQ=255)                                                                                                                 ggcggcGATGGGGCTCGCCTTGAGGACCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtc                                  <  3:263749/149‑1 (MQ=255)                                                                                                                  gcggcgATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtcg                                 >  3:89918/1‑149 (MQ=255)                                                                                                                   cggcgATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtcgt                                <  4:89918/149‑1 (MQ=255)                                                                                                                             gCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAg                      >  8:42505/1‑149 (MQ=255)                                                                                                                              cTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAgg                     <  5:339000/149‑1 (MQ=255)                                                                                                                                 gCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAggg                    <  3:168753/147‑1 (MQ=255)                                                                                                                                      cgtggCCAGTTCCCGGCGGAACTGGCCACGGTCGAACAGCTCGGGGGCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGCCCTGTCGGGGGCCGGTCAGGCCGAAGTGCGCGCGATGGTGTCGTCCTTGCGCAggggg                  >  3:281770/4‑142 (MQ=255)                                                                                                                                        gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgtg                                    <  6:95862/124‑1 (MQ=255)                                                                                                                                        gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgtg                                    >  5:95862/1‑124 (MQ=255)                                                                                                                                        gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgt                                     <  7:126645/123‑1 (MQ=255)                                                                                                                                        gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtct           >  3:8602/1‑149 (MQ=255)                                                                                                                                        gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtc            <  1:144129/148‑1 (MQ=255)                                                                                                                                        gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGACACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgt                                     >  8:126645/1‑123 (MQ=255)                                                                                                                                         ggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtctc          <  4:12731/149‑1 (MQ=255)                                                                                                                                                 ccGCCTGGAACTGGCCACGGTCGACCAGCTCGGGGTCCCCCTGGGGCCTCGCGTCACGGTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGAGGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAg  >  2:89859/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GCAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAG  >  NC_002947/1783884‑1784176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑