Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,784,033	T→C	6.5%	Q15R (CAG→CGG) ‡	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,784,033	0	T	C	6.5%	177.4 / 2.2	61	Q15R (CAG→CGG) ‡	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (28/29);  new base C (2/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

CAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAG  >  NC_002947/1783885‑1784176                                                                                                                                                     |                                                                                                                                                cAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCt                                                                                                                                                 <  2:32690/149‑1 (MQ=255)  aGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTg                                                                                                                                                <  6:191607/149‑1 (MQ=255)   ggATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCt                                                                                                                                                 <  1:306892/147‑1 (MQ=255)        ttGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTg                                                                                                                                          >  6:55068/1‑149 (MQ=255)          gTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTgg                                                                                                                                         <  4:182516/148‑1 (MQ=255)          gTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGc                                                                                                                                        >  4:130277/1‑149 (MQ=255)           tCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCTCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGcc                                                                                                                                       <  8:195224/149‑1 (MQ=255)           tCGACGAGCCAGGCGCGGGCCTCGATCACCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGc                                                                                                                                        >  1:200155/1‑148 (MQ=255)              acgaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGccct                                                                                                                                     >  1:150403/1‑146 (MQ=255)               cgaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACgg                                                                                                                                   >  3:206364/1‑149 (MQ=255)                gaGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCAGGCGGAACTGGccctcaa                                                                                                                                  <  2:150403/149‑6 (MQ=255)                       gcgcGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGACCAGTTCCGCCTGGAACTGGCCACGGTCGAACAg                                                                                                                           >  4:263749/1‑149 (MQ=255)                           gggCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCg                                                                                                                       <  4:267826/149‑1 (MQ=255)                             gCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCggg                                                                                                                     <  5:15526/149‑1 (MQ=255)                             gCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCggg                                                                                                                     <  5:244150/149‑1 (MQ=255)                              ccTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCgggg                                                                                                                    >  8:141356/1‑149 (MQ=255)                                        cGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTggg                                                                                                          <  2:191841/149‑1 (MQ=255)                                               aTGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATcgc                                                                                                   >  3:324739/1‑149 (MQ=255)                                                  tCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTc                                                                                                <  4:324739/149‑1 (MQ=255)                                                    gCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCAc                                                                                              <  7:141356/149‑1 (MQ=255)                                                          ggCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCt                                                                                        >  1:319758/1‑149 (MQ=255)                                                          ggCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCt                                                                                        <  4:206364/149‑1 (MQ=255)                                                          ggCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCt                                                                                        >  2:144129/1‑149 (MQ=255)                                                               gAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCgggg                                                                                                                    <  3:109151/116‑1 (MQ=255)                                                               gAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCgggg                                                                                                                    >  4:109151/1‑116 (MQ=255)                                                                aaaaCGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAAGAGCTCGGGGTCCACCTGGTGCATCGCGTCACGTTCCTGTCGTg                                                                                  <  1:222187/149‑1 (MQ=255)                                                                     gTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTg                                                                             >  6:339000/1‑149 (MQ=255)                                                                      tttGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTgg                                                                            <  3:130277/149‑1 (MQ=255)                                                                        tGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGc                                                                           <  2:319758/148‑1 (MQ=255)                                                                         gTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCt                                                                         >  3:12731/1‑149 (MQ=255)                                                                            gAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTc                                                                      >  6:182931/1‑149 (MQ=255)                                                                               cACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCggg                                                                   <  5:182931/149‑1 (MQ=255)                                                                                 ccTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCggg                                                                   >  7:1519/1‑147 (MQ=255)                                                                                 ccTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCggg                                                                   <  8:1519/147‑1 (MQ=255)                                                                                  cTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTg                                                                >  7:234038/1‑149 (MQ=255)                                                                                     gCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTc                                                                                     <  7:128435/125‑1 (MQ=255)                                                                                     gCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTc                                                                                     >  8:128435/1‑125 (MQ=255)                                                                                       cGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTc                                                           <  2:200155/149‑1 (MQ=255)                                                                                           cAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGc                                                                           >  7:2632/1‑129 (MQ=255)                                                                                           cAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGc                                                                           <  8:2632/129‑1 (MQ=255)                                                                                                ggATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGATCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAg                                                  >  2:8049/1‑149 (MQ=255)                                                                                                       tttCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCgg                                           >  4:168753/1‑149 (MQ=255)                                                                                                         gcTTGAAGGCGGCGATGGGGCGCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGGCCACCTGGGGCATCGCGTCACGTTCCTGTCATGGGGTGGCCTGTCGGTGTGCGGTCAGGCCGAAGTGCGCgg                                           <  7:52011/146‑1 (MQ=255)                                                                                                           ttGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                       >  6:156394/1‑149 (MQ=255)                                                                                                           ttGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATg                                       >  2:52590/1‑149 (MQ=255)                                                                                                             gAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCa                                                                                               >  4:153199/1‑91 (MQ=255)                                                                                                             gAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCa                                                                                               <  3:153199/91‑1 (MQ=255)                                                                                                                ggcggcGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtc                                  <  5:55068/149‑1 (MQ=255)                                                                                                                ggcggcGATGGGGCTCGCCTTGAGGACCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtc                                  <  3:263749/149‑1 (MQ=255)                                                                                                                 gcggcgATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtcg                                 >  3:89918/1‑149 (MQ=255)                                                                                                                  cggcgATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTgtcgt                                <  4:89918/149‑1 (MQ=255)                                                                                                                            gCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAg                      >  8:42505/1‑149 (MQ=255)                                                                                                                             cTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAgg                     <  5:339000/149‑1 (MQ=255)                                                                                                                                gCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAggg                    <  3:168753/147‑1 (MQ=255)                                                                                                                                     cgtggCCAGTTCCCGGCGGAACTGGCCACGGTCGAACAGCTCGGGGGCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGCCCTGTCGGGGGCCGGTCAGGCCGAAGTGCGCGCGATGGTGTCGTCCTTGCGCAggggg                  >  3:281770/4‑142 (MQ=255)                                                                                                                                       gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgtg                                    <  6:95862/124‑1 (MQ=255)                                                                                                                                       gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgtg                                    >  5:95862/1‑124 (MQ=255)                                                                                                                                       gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgt                                     <  7:126645/123‑1 (MQ=255)                                                                                                                                       gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtct           >  3:8602/1‑149 (MQ=255)                                                                                                                                       gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtc            <  1:144129/148‑1 (MQ=255)                                                                                                                                       gggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGACACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGgt                                     >  8:126645/1‑123 (MQ=255)                                                                                                                                        ggCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGAtctc          <  4:12731/149‑1 (MQ=255)                                                                                                                                                ccGCCTGGAACTGGCCACGGTCGACCAGCTCGGGGTCCCCCTGGGGCCTCGCGTCACGGTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGAGGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAg  >  2:89859/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                CAGGATATTGTCGACGAGCCAGGCGCGGGCCTCGATCAGCGGTCGGATGTCGCTGCCGGCGCGAAAACGTTTGTCGAGCACCTCGCCGGCCAGGCGGATGGCTTTCTTGAAGGCGGCGATGGGGCTCGCCTTGAGGGCCAGTTCCGCCTGGAACTGGCCACGGTCGAACAGCTCGGGGTCCACCTGGGGCATCGCGTCACGTTCCTGTCGTGGGGTGGCCTGTCGGGGTGCGGTCAGGCCGAAGTGCGCGGGATGGTGTCGTCCTTGCGCAGGGTGAAGATCTCGTAGCCAG  >  NC_002947/1783885‑1784176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑