Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,786,023 T→A 9.8% intergenic (+39/‑147) rpsB → / → tsf 30S ribosomal protein S2/elongation factor Ts

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,786,0230TA 9.8% 167.8 / 8.1 61intergenic (+39/‑147)rpsB/tsf30S ribosomal protein S2/elongation factor Ts
Reads supporting (aligned to +/- strand):  ref base T (29/26);  new base A (3/3);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

AGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAG  >  NC_002947/1785878‑1786160
                                                                                                                                                 |                                                                                                                                         
aGCTGTACATGACTTCGATGGCTCACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGc                                                                                                                                        >  3:259280/1‑149 (MQ=255)
  cTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGccc                                                                                                                                      <  4:151490/149‑1 (MQ=255)
    gTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGccccc                                                                                                                                    >  6:53053/1‑149 (MQ=255)
     tACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCt                                                                                                                                   >  3:270531/1‑149 (MQ=255)
      aCATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCt                                                                                                                                   <  5:53053/148‑1 (MQ=255)
         tGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCttttt                                                                                                                               <  2:53855/149‑1 (MQ=255)
           aCTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGc                                                                                                                             >  6:337638/1‑149 (MQ=255)
           aCTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCTTTTTGc                                                                                                                             <  1:275240/149‑1 (MQ=255)
                gATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCt                                                                                                                        >  6:312514/1‑149 (MQ=255)
                 aTGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCtt                                                                                                                       >  3:124718/1‑149 (MQ=255)
                     cTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGaaa                                                                                                                   <  4:124718/149‑1 (MQ=255)
                        aCGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATcc                                                                                                                >  3:98921/1‑149 (MQ=255)
                         cGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCct                                                                                                               <  7:339442/149‑1 (MQ=255)
                          gCAGTCATACGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGGTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGCGGGCTTTGCCCCCTTTTTGCCACCTTGAAAtaat                                                                                                               >  1:320634/1‑145 (MQ=255)
                          gCAGGCTTCCGCGGCCGCAACAACGTTGCCGGCGTCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCctg                                                                                                              <  4:259280/149‑1 (MQ=255)
                                aTCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCa                                                                                                        <  7:109907/149‑1 (MQ=255)
                                   cgcgGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCa                                                                                                     >  4:338293/1‑149 (MQ=255)
                                          gCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGccc                                                                                              >  7:238239/1‑149 (MQ=255)
                                          gCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGccc                                                                                              >  5:153467/1‑149 (MQ=255)
                                          gCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCTTTTTGcgt                                                                                                                           >  4:321950/1‑118 (MQ=255)
                                          gCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCAAAGCCCCCTTTTTGcgt                                                                                                                           <  3:321950/120‑3 (MQ=255)
                                               aacGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGcat                                                                                         >  7:45830/1‑149 (MQ=255)
                                               aacGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGcat                                                                                         >  3:257107/1‑149 (MQ=255)
                                                 cGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGcatca                                                                                       <  3:351859/149‑1 (MQ=255)
                                                   ttGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATc                                                                                                   <  1:31212/135‑1 (MQ=255)
                                                   ttGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATc                                                                                                   >  2:31212/1‑135 (MQ=255)
                                                   ttGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATg                                                                                     <  4:270531/149‑1 (MQ=255)
                                                       cggcggCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGcc                                                                                 <  2:76951/149‑1 (MQ=255)
                                                           ggcACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCctgct                                                                             <  6:235931/149‑1 (MQ=255)
                                                           ggcACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCctgct                                                                             <  5:337638/149‑1 (MQ=255)
                                                                cGAAGTTTACGCTGAAGAAGCGGATGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaat                                                                        <  6:255773/149‑1 (MQ=255)
                                                                   actttACGCTGCAGAAGCGGCTGCACCTGCTCCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTCGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCtgaaatgaa                                                                     <  4:229766/147‑1 (MQ=255)
                                                                   aGTTTACGCTGAAGAAGCGGCTGCACATGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAACGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCACCGCATCATGGGCCTGCtgaaatca                                                                      >  5:5461/1‑146 (MQ=255)
                                                                         cGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACTCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGc                                                               <  4:257107/149‑1 (MQ=255)
                                                                         cGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCAGCTGTCCGCATCGGCCCCCCATCATGGGCCTGCTGAAATGAAAACAGc                                                               >  2:170830/1‑149 (MQ=255)
                                                                                       cTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAAtt                                                 >  8:173685/1‑149 (MQ=255)
                                                                                               gctgctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGccc                                         >  2:247604/1‑149 (MQ=255)
                                                                                                   ctgAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGa                                     <  3:338293/149‑1 (MQ=255)
                                                                                                      aGTAATAGGCGCTAGCGTCTACTTGGCACTCAAATATGGGGCTTTGCCCCTTTTTTGCCAACTTTAAATCCTGCTGTCATCATCGGCCCCGATTCATGGTCCTGCTTAAATGAATACAGCGGTTTTGCAGAATTTAACGCCCGTTAt                                    <  2:320634/147‑1 (MQ=255)
                                                                                                      aGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGAtt                                   >  6:110520/1‑148 (MQ=255)
                                                                                                      aGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGAtt                                   >  6:366453/1‑148 (MQ=255)
                                                                                                       gTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTg                                  <  4:98921/148‑1 (MQ=255)
                                                                                                          aTAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAAAAGCGGATTTGCAGAATTGAACGCCCGTGATTGACgg                              >  5:215602/1‑149 (MQ=255)
                                                                                                             ggCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGt                            <  8:238239/148‑1 (MQ=255)
                                                                                                                 cTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAAt                       <  8:45830/149‑1 (MQ=255)
                                                                                                                  tAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATg                      >  7:315002/1‑149 (MQ=255)
                                                                                                                    gCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATg                                                                                     >  6:85825/1‑84 (MQ=255)
                                                                                                                    gCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATg                                                                                     <  5:85825/84‑1 (MQ=255)
                                                                                                                     cGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGtt                   <  7:173685/149‑1 (MQ=255)
                                                                                                                           cTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGaaaaa             >  6:158465/1‑149 (MQ=255)
                                                                                                                            ttGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGAtt                                   <  6:152714/126‑1 (MQ=255)
                                                                                                                            ttGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGAtt                                   >  5:152714/1‑126 (MQ=255)
                                                                                                                               gCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACttt         >  3:3611/1‑149 (MQ=255)
                                                                                                                                cACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTc        <  4:3611/149‑1 (MQ=255)
                                                                                                                                  ggCAAAAAGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTcc       >  3:194494/2‑148 (MQ=255)
                                                                                                                                   gCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTcc       >  4:86639/1‑147 (MQ=255)
                                                                                                                                     aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGAc                                <  7:106773/120‑1 (MQ=255)
                                                                                                                                     aaaaaGGGGGCAAAGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGAc                                >  8:106773/1‑120 (MQ=255)
                                                                                                                                       aaaGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAagag  >  7:296202/1‑148 (MQ=255)
                                                                                                                                           ggggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACgg                              >  6:359509/1‑116 (MQ=255)
                                                                                                                                           ggggCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACgg                              <  5:359509/116‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
AGCTGTACATGACTTCGATGGCTGACGCAGTCATCCGCGGCCGCAACAACGTTGCCGGCGGCACCGAAGTTTACGCTGAAGAAGCGGCTGCACCTGCTGCTGAGTAATAGGCGCTAGCGTCTACTTGGCACGCAAAAAGGGGGCTTTGCCCCCTTTTTGCCACCTTGAAATCCTGCTGTCAGCATCGGCCCCGCATCATGGGCCTGCTGAAATGAAAACAGCGGATTTGCAGAATTGAACGCCCGTGATTGACGGGTGGAATGGTTGAAAAACTTTCCAAGAG  >  NC_002947/1785878‑1786160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑