Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,786,944 A→G 8.3% T259A (ACC→GCC)  tsf → elongation factor Ts

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,786,9440AG 8.3% 129.1 / 2.9 48T259A (ACC→GCC) tsfelongation factor Ts
Reads supporting (aligned to +/- strand):  ref base A (20/24);  new base G (2/2);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CGGAAAACATCGTTGAGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAG  >  NC_002947/1786798‑1787081
                                                                                                                                                  |                                                                                                                                         
cGGAAAACATCGTTGAGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCAcc                                                                                                                                         <  8:95204/149‑1 (MQ=255)
cGGAAAACATCGTTGAGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCAcc                                                                                                                                         >  2:353063/1‑149 (MQ=255)
  gAAAACATCGTTGAGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTa                                                                                                                                       >  4:135499/1‑149 (MQ=255)
         tCGTTGAGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCaaa                                                                                                                                <  4:65837/149‑1 (MQ=255)
              gagaACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAg                                                                                                                            <  4:149792/148‑1 (MQ=255)
               agaACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAgg                                                                                                                           <  2:312694/148‑1 (MQ=255)
               agaACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGc                                                                                                                          >  6:18953/1‑149 (MQ=255)
               agaACATGATCAAAGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCa                                                                                                                                           >  6:147155/1‑132 (MQ=255)
               agaACATGATCAAAGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCa                                                                                                                                           <  5:147155/132‑1 (MQ=255)
                  aCATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGACTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCg                                                                                                                         >  6:236770/1‑147 (MQ=255)
                          aaCGGTCGTATCACCAAGTTCAATGCCGAAGCCTGGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCga                                                                                                               <  5:343282/149‑1 (MQ=255)
                          aaCGGTCGAATCACCAACTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCATCGTCATGAACCCGGAAATCAAGGTTCGCGAGCTGGCCAAGAACGCCGGTGCAGAAAACGTTTCCATCACCTACATCAAAGTACGCGAAGGCATCg                                                                                                                >  2:316245/1‑148 (MQ=255)
                             ggTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCgag                                                                                                              >  2:138041/1‑147 (MQ=255)
                               tCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGc                                                                                                          <  4:46917/149‑1 (MQ=255)
                                 gTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCa                                                                                                        <  5:18953/149‑1 (MQ=255)
                                    tCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCGCCTACTTTGAAGTAGGTGAAg                                                                                                                      >  3:134673/1‑132 (MQ=255)
                                    tCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCGCCTACTTTGAAGTAGGTGAAg                                                                                                                      <  4:134673/132‑1 (MQ=255)
                                         aaGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                 >  8:149213/1‑132 (MQ=255)
                                         aaGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                 <  7:149213/132‑1 (MQ=255)
                                          aGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCgacgac                                                                                               >  4:22635/1‑149 (MQ=255)
                                                 ggCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCa                                                                                                                                           <  3:166060/98‑1 (MQ=255)
                                                 ggCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCa                                                                                                                                           >  4:166060/1‑98 (MQ=255)
                                                             gCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCg                                                                            <  3:22635/149‑1 (MQ=255)
                                                                 aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCt                                                                          >  7:279093/1‑147 (MQ=255)
                                                                 aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCa                                                                        <  1:316245/149‑1 (MQ=255)
                                                                             ttCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGctgctg                                                             >  5:296778/1‑148 (MQ=255)
                                                                               cGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGctgctg                                                             <  6:296778/146‑1 (MQ=255)
                                                                                   aTGAACCCGGAAATCAAGGTTGGAGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCa                                                      <  2:311673/149‑1 (MQ=255)
                                                                                      aaCCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                    >  1:209978/1‑148 (MQ=255)
                                                                                       ccccGGATATCAAGGGTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGGCGCTGCTGCCAAGCAGTaa                                                  <  4:200159/148‑1 (MQ=255)
                                                                                       aCCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                    >  7:101875/1‑147 (MQ=255)
                                                                                         ccGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGa                                                <  1:138041/149‑1 (MQ=255)
                                                                                           ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACa                                              <  1:353063/149‑1 (MQ=255)
                                                                                           ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACa                                              <  2:209978/149‑1 (MQ=255)
                                                                                              aaTCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGcc                                           <  5:236770/149‑1 (MQ=255)
                                                                                              aaTCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGcc                                           <  1:170331/149‑1 (MQ=255)
                                                                                                              cTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                    >  5:299205/1‑124 (MQ=255)
                                                                                                              cTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                    <  6:299205/124‑1 (MQ=255)
                                                                                                                ggCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCa                            >  8:101686/1‑146 (MQ=255)
                                                                                                                 gCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCGGTCGCCCCaaa                          <  8:101875/147‑1 (MQ=255)
                                                                                                                   cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAgg                      >  2:214835/1‑149 (MQ=255)
                                                                                                                    aaGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGc                     >  4:298471/1‑149 (MQ=255)
                                                                                                                     aGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCgctgct                                                              >  8:273452/1‑107 (MQ=255)
                                                                                                                     aGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCgctgct                                                              <  7:273452/107‑1 (MQ=255)
                                                                                                                       aaaGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGc                  <  3:298471/149‑1 (MQ=255)
                                                                                                                        aaGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGcc                 <  7:101686/149‑1 (MQ=255)
                                                                                                                                 cAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAcgc        >  1:114755/1‑149 (MQ=255)
                                                                                                                                       tCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAg  >  8:212752/1‑149 (MQ=255)
                                                                                                                                         gTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAc          <  3:112794/139‑1 (MQ=255)
                                                                                                                                         gTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAc          >  4:112794/1‑139 (MQ=255)
                                                                                                                                              cTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCa   <  6:26976/141‑1 (MQ=255)
                                                                                                                                              cTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCa   >  5:26976/1‑141 (MQ=255)
                                                                                                                                                  |                                                                                                                                         
CGGAAAACATCGTTGAGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAG  >  NC_002947/1786798‑1787081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑