Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,786,961 C→T 8.0% G264G (GGC→GGT)  tsf → elongation factor Ts

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,786,9610CT 8.0% 142.3 / 3.3 50G264G (GGC→GGT) tsfelongation factor Ts
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base T (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGT  >  NC_002947/1786813‑1787106
                                                                                                                                                    |                                                                                                                                                 
agaACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGc                                                                                                                                                   >  6:18953/1‑149 (MQ=255)
   aCATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGACTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCg                                                                                                                                                  >  6:236770/1‑147 (MQ=255)
           aaCGGTCGTATCACCAAGTTCAATGCCGAAGCCTGGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCga                                                                                                                                        <  5:343282/149‑1 (MQ=255)
           aaCGGTCGAATCACCAACTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCATCGTCATGAACCCGGAAATCAAGGTTCGCGAGCTGGCCAAGAACGCCGGTGCAGAAAACGTTTCCATCACCTACATCAAAGTACGCGAAGGCATCg                                                                                                                                         >  2:316245/1‑148 (MQ=255)
              ggTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCgag                                                                                                                                       >  2:138041/1‑147 (MQ=255)
                tCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGc                                                                                                                                   <  4:46917/149‑1 (MQ=255)
                  gTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCa                                                                                                                                 <  5:18953/149‑1 (MQ=255)
                     tCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCGCCTACTTTGAAGTAGGTGAAg                                                                                                                                               <  4:134673/132‑1 (MQ=255)
                     tCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCGCCTACTTTGAAGTAGGTGAAg                                                                                                                                               >  3:134673/1‑132 (MQ=255)
                          aaGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                                          >  8:149213/1‑132 (MQ=255)
                          aaGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATc                                                                                                                                          <  7:149213/132‑1 (MQ=255)
                           aGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCgacgac                                                                                                                        >  4:22635/1‑149 (MQ=255)
                                              gCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCg                                                                                                     <  3:22635/149‑1 (MQ=255)
                                                  aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCt                                                                                                   >  7:279093/1‑147 (MQ=255)
                                                  aaaGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCa                                                                                                 <  1:316245/149‑1 (MQ=255)
                                                              ttCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGctgctg                                                                                      >  5:296778/1‑148 (MQ=255)
                                                                cGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGctgctg                                                                                      <  6:296778/146‑1 (MQ=255)
                                                                    aTGAACCCGGAAATCAAGGTTGGAGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCa                                                                               <  2:311673/149‑1 (MQ=255)
                                                                       aaCCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                                             >  1:209978/1‑148 (MQ=255)
                                                                        ccccGGATATCAAGGGTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGGCGCTGCTGCCAAGCAGTaa                                                                           <  4:200159/148‑1 (MQ=255)
                                                                        aCCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                                             >  7:101875/1‑147 (MQ=255)
                                                                          ccGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGa                                                                         <  1:138041/149‑1 (MQ=255)
                                                                            ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACa                                                                       <  2:209978/149‑1 (MQ=255)
                                                                            ggAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACa                                                                       <  1:353063/149‑1 (MQ=255)
                                                                               aaTCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGcc                                                                    <  1:170331/149‑1 (MQ=255)
                                                                               aaTCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGcc                                                                    <  5:236770/149‑1 (MQ=255)
                                                                                               cTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                                             <  6:299205/124‑1 (MQ=255)
                                                                                               cTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGt                                                                             >  5:299205/1‑124 (MQ=255)
                                                                                                 ggCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCa                                                     >  8:101686/1‑146 (MQ=255)
                                                                                                  gCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCGGTCGCCCCaaa                                                   <  8:101875/147‑1 (MQ=255)
                                                                                                    cAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAgg                                               >  2:214835/1‑149 (MQ=255)
                                                                                                     aaGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGc                                              >  4:298471/1‑149 (MQ=255)
                                                                                                      aGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCgctgct                                                                                       >  8:273452/1‑107 (MQ=255)
                                                                                                      aGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCgctgct                                                                                       <  7:273452/107‑1 (MQ=255)
                                                                                                        aaaGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGc                                           <  3:298471/149‑1 (MQ=255)
                                                                                                         aaGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGcc                                          <  7:101686/149‑1 (MQ=255)
                                                                                                                  cAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAcgc                                 >  1:114755/1‑149 (MQ=255)
                                                                                                                        tCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAg                           >  8:212752/1‑149 (MQ=255)
                                                                                                                          gTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAc                                   >  4:112794/1‑139 (MQ=255)
                                                                                                                          gTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAc                                   <  3:112794/139‑1 (MQ=255)
                                                                                                                               cTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCa                            <  6:26976/141‑1 (MQ=255)
                                                                                                                               cTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCa                            >  5:26976/1‑141 (MQ=255)
                                                                                                                                    ccTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCa                >  7:238045/1‑148 (MQ=255)
                                                                                                                                         ttCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgg          >  4:166139/1‑149 (MQ=255)
                                                                                                                                         ttCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgg          >  8:11063/1‑149 (MQ=255)
                                                                                                                                           cAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACggg         >  3:303841/1‑148 (MQ=255)
                                                                                                                                            aaaGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgggg        <  3:166139/148‑1 (MQ=255)
                                                                                                                                              aGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTa                                                                            <  4:205419/78‑1 (MQ=255)
                                                                                                                                              aGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTa                                                                            >  3:205419/1‑78 (MQ=255)
                                                                                                                                              aGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGggag      >  5:280085/1‑148 (MQ=255)
                                                                                                                                                  ggCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGt  <  3:135499/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGT  >  NC_002947/1786813‑1787106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑