Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,812,309 G→C 9.6% Q37E (CAG→GAG) ‡ PP_1615 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,812,3090GC 9.6% 156.0 / 7.0 52Q37E (CAG→GAG) ‡PP_1615LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (21/26);  new base C (2/3);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTTTGTCT  >  NC_002947/1812161‑1812450
                                                                                                                                                    |                                                                                                                                              
tCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTg                                                                                                                                                >  2:274327/1‑149 (MQ=255)
   cccATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCg                                                                                                                                             <  3:221288/149‑1 (MQ=255)
    ccATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCgg                                                                                                                                            >  3:130190/1‑149 (MQ=255)
       tGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGCAGATCGCCCGGCTg                                                                                                                                         <  4:29592/149‑1 (MQ=255)
         gcgcgcAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGAt                                                                                                                                       >  1:1402/1‑149 (MQ=255)
          cgcgcAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGCAGATCGCCCGGCTggag                                                                                                                                      <  7:72709/149‑4 (MQ=255)
             gcAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGc                                                                                                                                   <  7:42145/149‑1 (MQ=255)
                gcgcTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGAt                                                                                                                                >  4:46847/1‑149 (MQ=255)
                   cTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAg                                                                                                                             <  6:222393/149‑1 (MQ=255)
                          aCGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAccc                                                                                                                      >  4:177001/1‑149 (MQ=255)
                          aCGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAc                                                                                                                        <  8:61199/147‑1 (MQ=255)
                            ggCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAAccccc                                                                                                                    <  5:179591/149‑1 (MQ=255)
                                     gCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGCAGATCGCCCGGCTggaggac                                                                                                                                   >  8:188593/1‑119 (MQ=255)
                                     gCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTCCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGCAGATCGCCCGGCTggaggac                                                                                                                                   <  7:188593/125‑7 (MQ=255)
                                            ttGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTcggcggcgg                                                                                                    <  7:67578/149‑1 (MQ=255)
                                               gCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCCGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAg                                                                                                 <  4:130190/149‑1 (MQ=255)
                                                 aaTGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTg                                                                                               <  3:46847/149‑1 (MQ=255)
                                                     ctgcAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAAc                                                                                            <  2:292572/148‑1 (MQ=255)
                                                     ctgcAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACt                                                                                           >  6:221689/1‑149 (MQ=255)
                                                     ctgcAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACt                                                                                           >  1:219251/1‑149 (MQ=255)
                                                                             gCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACa                                                                   >  3:264293/1‑148 (MQ=255)
                                                                              cTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACaaa                                                                 >  7:309422/1‑149 (MQ=255)
                                                                                    gTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATtcgtc                                                           <  7:323462/149‑1 (MQ=255)
                                                                                       aCCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGaaa                                                                                                                         >  6:130679/1‑85 (MQ=255)
                                                                                       aCCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGaaa                                                                                                                         <  5:130679/85‑1 (MQ=255)
                                                                                       aCCCGGCGGGTGCTGCGATACAGCAGGCGCGGTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCACGCGGTCGGCGGCGGCAGTGAACTGGCCCGATTTCGGCCACGGCGACAAATtcgtcg                                                          <  7:220435/148‑1 (MQ=255)
                                                                                        cccGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGAt                                                        <  2:219251/148‑1 (MQ=255)
                                                                                                   ctgcGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCg                                            <  2:1402/149‑1 (MQ=255)
                                                                                                    tgcGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTc                                                                                                            >  1:299748/1‑85 (MQ=255)
                                                                                                    tgcGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTc                                                                                                            <  2:299748/85‑1 (MQ=255)
                                                                                                    tgcGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCAATGCCTTCCCATCgg                                           >  7:135504/1‑149 (MQ=255)
                                                                                                      cGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCTCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑tt                                                                                 >  7:313356/1‑109 (MQ=255)
                                                                                                      cGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCTCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑tt                                                                                 <  8:313356/109‑1 (MQ=255)
                                                                                                        aTACAGCAGGCGCGTTTGCAGTCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGgctgc                                       >  4:44792/1‑149 (MQ=255)
                                                                                                          aCAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCAc                                                                          <  4:285937/112‑1 (MQ=255)
                                                                                                          aCAGCAGGCGCGGTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCAc                                                                          >  3:285937/1‑112 (MQ=255)
                                                                                                            agcagGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCAt                                   <  8:309422/149‑1 (MQ=255)
                                                                                                             gcagGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATc                                  <  4:264293/149‑1 (MQ=255)
                                                                                                               agGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGTCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGa                                <  3:177001/149‑1 (MQ=255)
                                                                                                               agGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGa                                >  7:126015/1‑149 (MQ=255)
                                                                                                                   gcgTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTAt                            >  7:333488/1‑149 (MQ=255)
                                                                                                                      tttGCAGTCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATccc                         <  3:44792/149‑1 (MQ=255)
                                                                                                                         gCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTGCCATCGGCTGCTCAt                                   >  3:170231/1‑136 (MQ=255)
                                                                                                                         gCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCAt                                   <  4:170231/136‑1 (MQ=255)
                                                                                                                           aGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgtgt                    >  3:249691/1‑149 (MQ=255)
                                                                                                                            ggCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑GTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCtgtgtg                   >  2:284387/1‑149 (MQ=255)
                                                                                                                               ggTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTGCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCa                <  1:274327/149‑1 (MQ=255)
                                                                                                                                    atcAGCCGGCAGATCGCCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCaataat           >  4:171705/3‑149 (MQ=255)
                                                                                                                                     ccAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATg          >  6:237826/1‑149 (MQ=255)
                                                                                                                                        gCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGttt       <  5:221689/149‑1 (MQ=255)
                                                                                                                                           gggCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTTTGt    <  8:126015/149‑1 (MQ=255)
                                                                                                                                           gggCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTTTGt    <  8:333488/149‑1 (MQ=255)
                                                                                                                                             gCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTTTGTCt  >  7:26207/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TCGCCCATGGCGCGCAGCGCTTCCTCACGGCCATCCTGCAAGCGTTGGCAATGCTGCAGGAAGGTCTGCCCGGCTTCGCTCAGGGTTACCCGGCGGGTGCTGCGATACAGCAGGCGCGTTTGCAGGCGGTCCTCCAGCCGGGCGATCTGCCGGCTGATGTGCGATGAGGAAACCCCCAGGCGTTCGGCGGCGGCAGTGAACTGGCCCGA‑TTCGGCCACGGCGACAAATTCGTCGATGCCTTCCCATCGGCTGCTCATCGATTATCCCTGTGTGGCAATAATGTTTTGTCT  >  NC_002947/1812161‑1812450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑