Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,835,835 A→T 13.7% L152* (TTG→TAG) ‡ yohC ← inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,835,8350AT13.7% 120.7 / 8.9 50L152* (TTG→TAG) ‡yohCinner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (23/20);  new base T (4/3);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATGCATTGCGCC  >  NC_002947/1835691‑1835978
                                                                                                                                                |                                                                                                                                               
tAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGcc                                                                                                                                             >  8:69473/1‑149 (MQ=255)
  gTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGACACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCAGACGGTATGTTCATGAACGTCGGCAAGCCGa                                                                                                                                           >  3:242691/1‑149 (MQ=255)
      gtgtAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACAt                                                                                                                                        <  2:195639/148‑1 (MQ=255)
         tAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCTTGCCGAcgttcat                                                                                                                                    <  2:309919/149‑7 (MQ=255)
         tAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCTTGCCGAcgttca                                                                                                                                     <  6:367556/148‑6 (MQ=255)
           gACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATAcag                                                                                                                                    >  1:305804/1‑147 (MQ=255)
                   ccACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACAcc                                                                                                                          >  4:276532/1‑149 (MQ=255)
                        cacAGTCCCCAGATAATCAGGGTAGCGTCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTTTATAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTa                                                                                                                     <  4:242691/147‑1 (MQ=255)
                               cccAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATg                                                                                                              >  1:358188/1‑149 (MQ=255)
                                  aGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATggcg                                                                                                           <  3:276532/149‑1 (MQ=255)
                                     tAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAACAGATGGCGGCa                                                                                                        <  6:59576/149‑1 (MQ=255)
                                        tCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTg                                                                                                     >  1:210288/1‑149 (MQ=255)
                                                gCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCa                                                                                              <  7:69473/148‑1 (MQ=255)
                                                     cATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGc                                                                                        >  1:179877/1‑149 (MQ=255)
                                                                    tactacCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCg                                                                         >  1:20603/1‑149 (MQ=255)
                                                                      ctacCAAGCCCACTGCCAGCACCGAGCTTGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCgg                                                                        <  2:179877/148‑1 (MQ=255)
                                                                       tacCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCggg                                                                       >  2:27141/1‑148 (MQ=255)
                                                                       tacCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCggg                                                                       >  2:286279/1‑148 (MQ=255)
                                                                       tacCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCggg                                                                       >  1:53970/1‑148 (MQ=255)
                                                                         ccAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTa                                                                     >  2:144170/1‑148 (MQ=255)
                                                                                  cTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGcgccgc                                                            >  8:155806/1‑148 (MQ=255)
                                                                                   ctccAGCTCGGTGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGcgccgc                                                            >  6:369230/3‑147 (MQ=255)
                                                                                     ccAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCa                                                          <  2:210288/147‑1 (MQ=255)
                                                                                         cACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAgcg                                                                >  5:252954/1‑137 (MQ=255)
                                                                                         cACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAgcg                                                                <  6:252954/137‑1 (MQ=255)
                                                                                                 tGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGcc                                                                                       <  7:222820/106‑1 (MQ=255)
                                                                                                 tGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGcc                                                                                       >  8:222820/1‑106 (MQ=255)
                                                                                                 tGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGaa                                             >  5:222758/1‑148 (MQ=255)
                                                                                                  ggAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAAc                                            >  8:65104/1‑148 (MQ=255)
                                                                                                    agaACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAg                                         <  7:155806/149‑1 (MQ=255)
                                                                                                               ccTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGgt                              <  2:305804/149‑1 (MQ=255)
                                                                                                               ccTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGgt                              <  2:20603/149‑1 (MQ=255)
                                                                                                                cTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGc                                                                                                                   >  4:229972/1‑63 (MQ=255)
                                                                                                                cTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGc                                                                                                                   <  3:229972/63‑1 (MQ=255)
                                                                                                                       cGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTgg                      <  6:222758/149‑1 (MQ=255)
                                                                                                                           ggTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCTCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCaa                   >  2:39902/1‑148 (MQ=255)
                                                                                                                                 ttCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGa             <  2:358188/148‑1 (MQ=255)
                                                                                                                                 ttCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGAt            <  1:27141/149‑1 (MQ=255)
                                                                                                                                   cATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATg           <  1:144170/148‑1 (MQ=255)
                                                                                                                                    ctgtatgTCGGCTTGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATGCa         >  3:30008/7‑149 (MQ=255)
                                                                                                                                     tgtatgTCGGCTTGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCg                                >  5:186668/6‑125 (MQ=255)
                                                                                                                                     tgtatgTCGGCTTGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCg                                <  6:186668/120‑1 (MQ=255)
                                                                                                                                     tgtatgTCGGCTTGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATGCAt        >  1:100997/6‑149 (MQ=255)
                                                                                                                                     tGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGAt            <  8:11901/145‑1 (MQ=255)
                                                                                                                                     tGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGAt            >  7:11901/1‑145 (MQ=255)
                                                                                                                                      gAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGa             <  7:220000/143‑1 (MQ=255)
                                                                                                                                      gAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGa             >  8:220000/1‑143 (MQ=255)
                                                                                                                                         tgTCGGCTTGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATGCATTgcg    >  3:31354/2‑149 (MQ=255)
                                                                                                                                               cAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATGCATTGCGcc  >  7:126495/1‑145 (MQ=255)
                                                                                                                                               cAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATGCATTGCGcc  <  8:126495/145‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TAGTTGGTGTAGACGGGCCCCACGCCCAGTCCCCAGATAATCACGGTAGCGGCCATGATTGCCACCAGTACTACCAAGCCCACTGCCAGCACCGAGCTGGAGAACAGGAAGCCTTCATCCGACGGTATGTTCATGAACGTCGGCAAGCCGACATACAGCAGGTACACCGTGTAGCAGATGGCGGCAGTGCCGATCAGCATGCCCAGCCACAGGTGCGGGTAAAGCGCCGCCAGGCCGCCGATGAACAAGGGGGTGGCGGTGTAAGTGGCAAAAGCGATGCATTGCGCC  >  NC_002947/1835691‑1835978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑