Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,997,790 G→A 8.0% A198V (GCA→GTA)  rfbD ← dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,997,7900GA 8.0% 144.1 / 2.0 50A198V (GCA→GTA) rfbDdTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base A (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

ACTTTCAGCGCTACGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCAGCTGGTGCGCAG  >  NC_002947/1997643‑1997935
                                                                                                                                                   |                                                                                                                                                 
aCTTTCAGCGCTACGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGAtgtgtg                                                                                                                                                   <  7:82855/148‑1 (MQ=255)
aCTTTCAGCGCTACGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGAtgtgtg                                                                                                                                                   <  6:128959/148‑1 (MQ=255)
       gcgcTACGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACg                                                                                                                                           <  3:212741/149‑1 (MQ=255)
        cgcTACGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGAtgtgtg                                                                                                                                                   >  4:168843/1‑140 (MQ=255)
        cgcTACGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGAtgtgtg                                                                                                                                                   <  3:168843/140‑1 (MQ=255)
             cGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAACCCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCgg                                                                                                                                       >  1:2842/1‑147 (MQ=255)
             cGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAACCCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCgg                                                                                                                                       <  2:2842/147‑1 (MQ=255)
                  gTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAg                                                                                                                                <  3:101492/149‑1 (MQ=255)
                     cGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTa                                                                                                                                              >  5:370623/1‑132 (MQ=255)
                     cGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTa                                                                                                                                              <  6:370623/132‑1 (MQ=255)
                      gTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAg                                                                                                                                <  2:250577/145‑1 (MQ=255)
                      gTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAg                                                                                                                                >  1:250577/1‑145 (MQ=255)
                       ttGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAg                                                                                                                           >  4:292491/1‑149 (MQ=255)
                          aGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAgcgc                                                                                                                        <  5:281222/149‑1 (MQ=255)
                            cATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGACAAGTGGTAAATCCCTGCCAAGTGACCGTTGTCTTGCCCATTGAAGAATTGCCGCATGATGTGTGCAATAACCTAGGCGATGATGTAAGCGccc                                                                                                                      >  1:273474/1‑149 (MQ=255)
                                cgcCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTgg                                                                                                                  <  8:242899/149‑1 (MQ=255)
                                      cACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTgcgc                                                                                                            <  3:292491/149‑1 (MQ=255)
                                                 cAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCt                                                                                                 >  6:152002/1‑149 (MQ=255)
                                                    aaCCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGc                                                                                               >  2:179153/1‑148 (MQ=255)
                                                        aTGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCa                                                                                          >  2:243495/1‑149 (MQ=255)
                                                            cAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTCCTACCACGCt                                                                                      >  1:135869/1‑149 (MQ=255)
                                                                   tttCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGt                                                                                >  8:183658/1‑148 (MQ=255)
                                                                     tCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGtct                                                                              <  5:152002/148‑1 (MQ=255)
                                                                      cACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGtctc                                                                             <  8:315547/148‑1 (MQ=255)
                                                                       aCCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCAc                                                                           >  4:173885/1‑149 (MQ=255)
                                                                           gACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTc                                                                       <  3:173885/149‑1 (MQ=255)
                                                                              gCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGtct                                                                              >  3:308845/1‑139 (MQ=255)
                                                                              gCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGtct                                                                              <  4:308845/139‑1 (MQ=255)
                                                                                    aaGTGGTAAACCCCGCCCCGGGGACGGGTGTCTGGCCCATTGACGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAg                                                              <  2:135869/149‑1 (MQ=255)
                                                                                     aGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAgg                                                             >  7:246644/1‑149 (MQ=255)
                                                                                     aGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAgg                                                             <  2:273474/149‑1 (MQ=255)
                                                                                           aaaTCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTg                                                                                                     >  3:302061/1‑103 (MQ=255)
                                                                                           aaaTCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTg                                                                                                     <  4:302061/103‑1 (MQ=255)
                                                                                            aaTCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCgcagca                                                      <  1:243495/149‑1 (MQ=255)
                                                                                              tCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCAc                                                                           >  4:331031/1‑126 (MQ=255)
                                                                                              tCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCAc                                                                           <  3:331031/126‑1 (MQ=255)
                                                                                                 cTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTc                                                 <  1:179153/149‑1 (MQ=255)
                                                                                                       ggTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCa                                           <  2:109778/149‑1 (MQ=255)
                                                                                                                      gCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCcgcgc                            >  6:122477/1‑149 (MQ=255)
                                                                                                                      gCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCcgcgc                            >  2:312306/1‑149 (MQ=255)
                                                                                                                       cccATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCcgcgcg                           >  3:92031/1‑149 (MQ=255)
                                                                                                                          aTTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCgcagc                                                       >  2:288368/1‑118 (MQ=255)
                                                                                                                          aTTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCgcagc                                                       <  1:288368/118‑1 (MQ=255)
                                                                                                                              aaGATTTGCCGCAGGATGTGTACAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCaaaa                                        <  7:211639/129‑1 (MQ=255)
                                                                                                                              aaGATTTGCCGCAGGATGTGTACAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCaaaa                                        <  7:217257/129‑1 (MQ=255)
                                                                                                                              aaGATTTGCCGCAGGATGTGTACAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCaaaa                                        >  8:211639/1‑129 (MQ=255)
                                                                                                                              aaGATTTGCCGCAGGATGTGTACAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCaaaa                                        >  8:217257/1‑129 (MQ=255)
                                                                                                                                    tGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTAAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACAcc                >  5:93149/1‑147 (MQ=255)
                                                                                                                                     gCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCa              <  7:183658/148‑1 (MQ=255)
                                                                                                                                     gCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCa              <  4:92031/148‑1 (MQ=255)
                                                                                                                                      ccGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCAg             <  1:312306/148‑1 (MQ=255)
                                                                                                                                       cGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCAGCt           >  5:95018/1‑149 (MQ=255)
                                                                                                                                       cGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCAGCt           >  8:306676/1‑149 (MQ=255)
                                                                                                                                        gCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCAGCTg          >  6:34439/1‑149 (MQ=255)
                                                                                                                                                 gtgCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCACGCCCAGCATGGTCTTGGCAAAATTGTGCCCACGCGCAGCATACACCCAGCTGGTGCGCAg  >  3:99665/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACTTTCAGCGCTACGCCAGTGCGTTGAGCATGCGCCAGCACGAACTGAGCAAAACCATGCCAAGAGGTTTCACCGGACGCAGCCAAGTGGTAAATCCCTGCCAGGTGACGGTTGTCTTGCCCATTGAAGATTTGCCGCAGGATGTGTGCAGTAACGTCGGCGATCAGGTCAGCGCCCGTGGGTGCGCCAAATTGGTCTGCTACCACGCTCAACGTCTCACGCTCCGCCGCCAGGCGCAGCATGGTCTTGGCAAAATTGTGCCCGCGCGCAGCATACACCCAGCTGGTGCGCAG  >  NC_002947/1997643‑1997935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑