Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 132,463 G→A 10.4% intergenic (+37/‑115) cc → / → dsbA cytochrome c4/thiol:disulfide interchange protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947132,4630GA10.4% 126.5 / 4.7 48intergenic (+37/‑115)cc/dsbAcytochrome c4/thiol:disulfide interchange protein
Reads supporting (aligned to +/- strand):  ref base G (24/19);  new base A (3/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.74e-01

AACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGG  >  NC_002947/132322‑132608
                                                                                                                                             |                                                                                                                                                 
aaCCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGc                                                                                                                                            >  5:19104/1‑149 (MQ=255)
      aCCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCt                                                                                                                                      >  7:23212/1‑149 (MQ=255)
         aaCGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTCGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCtttt                                                                                                                                   <  6:345486/149‑1 (MQ=255)
             aTGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCtttcatca                                                                                                                               <  5:19718/149‑6 (MQ=255)
               ggCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgt                                                                                                                             >  5:203887/1‑149 (MQ=255)
               ggCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgt                                                                                                                             >  8:145716/1‑149 (MQ=255)
                gCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgtc                                                                                                                            <  4:352391/149‑1 (MQ=255)
                       cATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGGTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGACCTTTTTTCCGTCCGCAGcc                                                                                                                     >  4:341044/1‑149 (MQ=255)
                       cATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGGTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCGTTTTTTCCGTCCGCAGcc                                                                                                                     >  4:42645/1‑149 (MQ=255)
                            ccATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTac                                                                                                                <  6:6708/149‑1 (MQ=255)
                             cATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTaca                                                                                                               >  7:214508/1‑149 (MQ=255)
                                    aCCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTACCGTCCGCAGCCGTTACACTACAg                                                                                                         >  7:319466/1‑148 (MQ=255)
                                     ccATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGaa                                                                                                       <  6:284239/149‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgtc                                                                                                                            >  2:338962/1‑119 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgtc                                                                                                                            <  1:338962/119‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGc                                                                                                <  8:264408/147‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGc                                                                                                <  6:47684/147‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCtttc                                                                                                                                   <  7:325336/112‑2 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCtttc                                                                                                                                   >  8:325336/1‑111 (MQ=255)
                                                      aGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCg                                                                                        <  6:19104/147‑1 (MQ=255)
                                                        cAACCATGACATCGAAGCGTAGGCCAGCTACATCCAGGGCCTGCAATAACTTTCGGTTAAAGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTCCCCTTTTTTCCATACGCAGCCGTTAAACTACAGAACACCAGCCCTTCCAGGcat                                                                                    >  3:5076/1‑147 (MQ=255)
                                                             aTGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGtct                                                                                >  1:99721/1‑148 (MQ=255)
                                                              tGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGa                                                                                                  >  2:201197/1‑129 (MQ=255)
                                                              tGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCCTCCGCAGCCGTTACACTACAGAACTCGa                                                                                                  <  1:201197/129‑1 (MQ=255)
                                                                aCATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGt                                                                            >  1:94804/1‑149 (MQ=255)
                                                                  aTCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGc                                                                          <  3:243940/149‑1 (MQ=255)
                                                                   tCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTg                                                                           >  3:333093/1‑147 (MQ=255)
                                                                     gAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAAGTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCAGCAGCCGTTACACTACAGAACTCGCGCCATTCCCGCCCTTTCTCAGTGCAgg                                                                       >  3:264066/1‑149 (MQ=255)
                                                                     gAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAgg                                                                       >  6:164306/1‑149 (MQ=255)
                                                                       aGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTc                                                                     <  6:203887/149‑1 (MQ=255)
                                                                              ggaaGCTTCATCCAGGGGCTGCATTAACTTTCGTTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGcgtcgt                                                              <  4:5076/146‑1 (MQ=255)
                                                                                      cATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGAcc                                                       >  1:216409/1‑148 (MQ=255)
                                                                                        tCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCAt                                                    <  2:94804/149‑1 (MQ=255)
                                                                                                   cATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAg                                          >  7:107245/1‑148 (MQ=255)
                                                                                                   cATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGATACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAg                                          >  3:142440/1‑148 (MQ=255)
                                                                                                       aaCTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTACCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGt                                      <  8:319466/148‑1 (MQ=255)
                                                                                                         cTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTaaa                                   <  8:214508/149‑1 (MQ=255)
                                                                                                          tttCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCGTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAg                                  >  5:265503/1‑149 (MQ=255)
                                                                                                             cGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCa                                <  7:145716/148‑1 (MQ=255)
                                                                                                                  aaTGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGt                           >  4:264569/1‑148 (MQ=255)
                                                                                                                   aTGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTaa                         <  6:265503/149‑1 (MQ=255)
                                                                                                                   aTGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTa                          >  1:296341/1‑148 (MQ=255)
                                                                                                                         tAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGAt                   >  3:274620/1‑149 (MQ=255)
                                                                                                                             tCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATtctc               <  2:99721/149‑1 (MQ=255)
                                                                                                                                   aaaaaaGGGCAGCCCGGCCGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAgcgct         >  4:143020/4‑149 (MQ=255)
                                                                                                                                    aaaaaGGGCAGCCCGGCCGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAgcgctg        >  4:44626/3‑149 (MQ=255)
                                                                                                                                     gaAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAgcgctgc       >  7:40126/1‑149 (MQ=255)
                                                                                                                                         gggCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGcgctgcgctg   <  8:40126/149‑1 (MQ=255)
                                                                                                                                          ggcggcCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTgg  <  2:296341/149‑1 (MQ=255)
                                                                                                                                             ggcCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAAc                       <  3:240061/125‑1 (MQ=255)
                                                                                                                                             ggcCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAAc                       >  4:240061/1‑125 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
AACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGG  >  NC_002947/132322‑132608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑