Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,028,080 T→C 7.3% A290A (GCT→GCC)  wbpL → glycosyl transferase WbpL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,028,0800TC 7.3% 154.5 / 3.0 55A290A (GCT→GCC) wbpLglycosyl transferase WbpL
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base C (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

GGGTGTATTCGTGGTTGATGCCACGTTTACCTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGCTGGGGAGCAAGAATAGGATGGCCAA  >  NC_002947/2027933‑2028224
                                                                                                                                                   |                                                                                                                                                
gggTGTATTCGTGGTTGATGCCACGTTTACCTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCt                                                                                                                                                  <  3:107338/148‑1 (MQ=255)
          gTGGTTGATGCCACGTTTACCTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGt                                                                                                                                       >  3:193445/1‑149 (MQ=255)
                gATGCCACGTTTACCTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGc                                                                                                                                  >  2:231857/1‑148 (MQ=255)
                     cACGTTTACCTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCtactac                                                                                                                            >  5:270449/1‑149 (MQ=255)
                              cTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCt                                                                                                                                     <  8:14371/131‑1 (MQ=255)
                              cTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCt                                                                                                                                     >  7:14371/1‑131 (MQ=255)
                              cTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGccc                                                                                                                   >  5:255045/1‑149 (MQ=255)
                                  gcgcggcgcCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGc                                                                                                               >  6:54698/1‑149 (MQ=255)
                                     cggcgcCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGt                                                                                                            <  4:26969/149‑1 (MQ=255)
                                      gtcgcCTGCTGCGCGGTGAGAAGGTTTATCAGGCGCATTGCTGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTTGCTGCTATTAACCTGGTCTGGCTACTACCGGTGGCCATGGCGGTa                                                                                                           <  2:157594/147‑1 (MQ=255)
                                      ggcgcCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTa                                                                                                           <  1:55005/149‑1 (MQ=255)
                                          ccTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGt                                                                                                         <  3:268984/147‑1 (MQ=255)
                                          ccTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGt                                                                                                         >  4:268984/1‑147 (MQ=255)
                                            tgctgcGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTgg                                                                                                                                        <  8:192723/114‑1 (MQ=255)
                                            tgctgcGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTgg                                                                                                                                        >  7:192723/1‑114 (MQ=255)
                                            tgctgcGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTgg                                                                                                                                   <  5:40221/119‑1 (MQ=255)
                                            tgctgcGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTgg                                                                                                                                   >  6:40221/1‑119 (MQ=255)
                                                    ggTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCt                                                                                             <  1:231857/149‑1 (MQ=255)
                                                     gTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTc                                                                                            <  7:296737/149‑1 (MQ=255)
                                                        aCAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGa                                                                                          <  6:270449/148‑1 (MQ=255)
                                                         cAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATg                                                                                        >  6:250068/1‑149 (MQ=255)
                                                          aaGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATgg                                                                                       >  7:6866/1‑149 (MQ=255)
                                                          aaGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATgg                                                                                       >  4:172606/1‑149 (MQ=255)
                                                          aaGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCCATTa                                                                                                                                              <  4:344920/94‑1 (MQ=255)
                                                          aaGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCCATTa                                                                                                                                              >  3:344920/1‑94 (MQ=255)
                                                          aaGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCCATTa                                                                                                                                              <  2:101047/94‑1 (MQ=255)
                                                          aaGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCCATTa                                                                                                                                              >  1:101047/1‑94 (MQ=255)
                                                          aaGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCAGCATGGTCACCATCGACCGGTAAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATgg                                                                                       >  8:296662/1‑149 (MQ=255)
                                                            ggTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGgcg                                                                                     >  4:29340/1‑149 (MQ=255)
                                                               ttATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTg                                                                                                  <  8:90983/133‑1 (MQ=255)
                                                               ttATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTg                                                                                                  >  7:90983/1‑133 (MQ=255)
                                                                 aTCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCAGCATGGTCACCATCGACCGGTAAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACt                                                                                 <  7:296662/148‑1 (MQ=255)
                                                                     ggCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGaa                                                                            >  7:311899/1‑149 (MQ=255)
                                                                       cgcATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAAcc                                                                          <  3:29340/149‑1 (MQ=255)
                                                                       cgcATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAAcc                                                                          <  4:132886/149‑1 (MQ=255)
                                                                        gcATCGCAGCCTTTCTTTTCAGTTTGCGGCTCGCCGGCATGGTCACCATAGAGCGGTGTGCCTGGCCGTGTCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCTATGGCGCACTGTGaagc                                                                          <  4:193445/148‑3 (MQ=255)
                                                                               aGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGc                                                                  <  8:311899/149‑1 (MQ=255)
                                                                                                 gCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATAtt                                                >  1:135196/1‑149 (MQ=255)
                                                                                                  cGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTg                                               <  6:255045/149‑1 (MQ=255)
                                                                                                   ggCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGc                                              >  3:72333/1‑149 (MQ=255)
                                                                                                        gCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTgg                                           <  5:54698/147‑1 (MQ=255)
                                                                                                        gCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTgg                                           <  2:135196/147‑1 (MQ=255)
                                                                                                                 gTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTAt                                >  3:150471/1‑149 (MQ=255)
                                                                                                                      cATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCtt                                                                >  7:18029/1‑112 (MQ=255)
                                                                                                                      cATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCtt                                                                <  8:18029/112‑1 (MQ=255)
                                                                                                                      cATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGc                           >  5:14182/1‑149 (MQ=255)
                                                                                                                      cATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGc                           >  6:24221/1‑149 (MQ=255)
                                                                                                                        tCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGCTg                         <  8:6866/149‑1 (MQ=255)
                                                                                                                             cGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATg                                                                                        >  4:293719/1‑81 (MQ=255)
                                                                                                                                tGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGCTggg                       <  3:201684/143‑1 (MQ=255)
                                                                                                                                tGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGCTggg                       >  4:201684/1‑143 (MQ=255)
                                                                                                                                      tGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGt                                                                                                    <  6:259489/60‑1 (MQ=255)
                                                                                                                                      tGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGt                                                                                                    >  5:259489/1‑60 (MQ=255)
                                                                                                                                        gCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGCTGGGGAGCAAGAATAGGa         <  5:24221/149‑1 (MQ=255)
                                                                                                                                        gCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAAGCTGGGGAGCAAGAATAGGa         >  8:205796/1‑149 (MQ=255)
                                                                                                                                               cggcTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGCTGGGGAGCAAGAATAGGATGGCCaa  <  5:250068/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GGGTGTATTCGTGGTTGATGCCACGTTTACCTTGGCGCGGCGCCTGCTGCGCGGTGACAAGGTTTATCAGGCGCATCGCAGCCATGCTTATCAGTATGCGGCTCGCCGGCATGGTCACCATCGACCGGTGAGCCTGGCCGTGGCGGCTATTAACCTGGTCTGGCTACTACCGGTGGCCCTGGCGGTAGTGCTGTTGAACCTCGATGGCGCACTGGGAACCCTGTTCGCTTATGTGCCTCTGATATTGCTGGCAATCACCTATCAGGCTGGGGAGCAAGAATAGGATGGCCAA  >  NC_002947/2027933‑2028224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑