Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,043,319 G→A 6.1% R196H (CGC→CAC)  PP_1817 → short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,043,3190GA 6.1% 197.5 / 2.1 65R196H (CGC→CAC) PP_1817short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (32/29);  new base A (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

AGGGGCGGCGCCAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCGGGCATTG  >  NC_002947/2043176‑2043460
                                                                                                                                               |                                                                                                                                             
aGGGGCGGCGCCAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCcgcg                                                                                                                                          >  7:262605/1‑149 (MQ=255)
  gggCGGCGCCAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACTCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCt                                                                                                                                        <  5:369120/149‑1 (MQ=255)
   ggcggcGCCAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCtt                                                                                                                                       <  1:52239/149‑1 (MQ=255)
    gcggcgCCAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTa                                                                                                                                      >  4:245338/1‑149 (MQ=255)
      ggcgCCAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTAcc                                                                                                                                    <  8:262605/149‑1 (MQ=255)
           cAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGcc                                                                                                                               >  3:335901/1‑149 (MQ=255)
                  gAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCcgc                                                                                                                        >  5:175362/1‑149 (MQ=255)
                  gAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCcgc                                                                                                                        >  7:23003/1‑149 (MQ=255)
                       tCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAg                                                                                                                   >  6:186328/1‑149 (MQ=255)
                        cTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAgg                                                                                                                  >  5:351629/1‑149 (MQ=255)
                                   gcaagcaTGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTcg                                                                                                         >  3:159762/1‑147 (MQ=255)
                                   gcaagcaTGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTcg                                                                                                         <  4:159762/147‑1 (MQ=255)
                                                 aTCGTTCTGACCAAGTCTGCGGCCATCGAGTACGCCAAGTAGGGCATCAGCGTGAACGCCGTGTGCCCGGCCTTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCAcc                                                                                          <  6:351629/148‑1 (MQ=255)
                                                   cGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACTGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCAcc                                                                                          <  6:143221/146‑1 (MQ=255)
                                                   cGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACTGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCAcc                                                                                          >  5:143221/1‑146 (MQ=255)
                                                          aCCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAggg                                                                                  <  4:335901/147‑1 (MQ=255)
                                                          aCCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGgcg                                                                                >  2:49635/1‑149 (MQ=255)
                                                           ccAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAgg                                                                                   >  2:326050/1‑145 (MQ=255)
                                                           ccAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAgg                                                                                   <  1:326050/145‑1 (MQ=255)
                                                              aGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCAtt                                                                            >  8:262031/1‑149 (MQ=255)
                                                               gTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTg                                                                           <  1:25815/149‑1 (MQ=255)
                                                               gTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCACGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTg                                                                           <  3:245338/149‑1 (MQ=255)
                                                                  ggcggcCATCGAGTACGCCAAGAAGGTCATCCGGGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCGGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGc                                                                         <  7:145244/148‑1 (MQ=255)
                                                                     ggcCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAgg                                                                     >  5:196851/1‑149 (MQ=255)
                                                                     ggcCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAgg                                                                     >  4:118499/1‑149 (MQ=255)
                                                                      gcCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGt                                                                    <  5:186328/149‑1 (MQ=255)
                                                                       ccATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTc                                                                   >  8:199210/1‑149 (MQ=255)
                                                                        cATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTc                                                                   >  3:282017/1‑148 (MQ=255)
                                                                        cATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTc                                                                   >  6:365067/1‑148 (MQ=255)
                                                                         aTCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGa                                                                 <  6:196851/149‑1 (MQ=255)
                                                                          tCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAg                                                                <  5:365067/149‑1 (MQ=255)
                                                                               tACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCACCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAAt                                                           >  7:316325/1‑149 (MQ=255)
                                                                                 cGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAggg                                                                                  <  8:186214/124‑1 (MQ=255)
                                                                                 cGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAggg                                                                                  >  7:186214/1‑124 (MQ=255)
                                                                                  gCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCTGCGCTTACCAGGCCGAGCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGaaaacat                                                        >  7:233170/1‑145 (MQ=255)
                                                                                  gCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGc                                                        <  1:49635/149‑1 (MQ=255)
                                                                                           ggCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGt                                               <  1:213994/149‑1 (MQ=255)
                                                                                            gCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTg                                              >  1:84141/1‑149 (MQ=255)
                                                                                               tCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTg                                           >  8:39940/1‑149 (MQ=255)
                                                                                                       aaCGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCtgtg                                   <  6:175362/149‑1 (MQ=255)
                                                                                                        aCGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCtgtg                                   >  8:74579/1‑148 (MQ=255)
                                                                                                             gtgtGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCACGCCGACCCACGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGa                             >  4:253148/1‑149 (MQ=255)
                                                                                                                tgCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGAcgg                          <  8:23003/149‑1 (MQ=255)
                                                                                                                        cGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGCAAATCGCCAGCGCCGTGCTGTATCTGTGCa                                 <  1:33028/134‑1 (MQ=255)
                                                                                                                              cGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGAcggcgcggc                    >  3:317224/1‑141 (MQ=255)
                                                                                                                              cGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGAcggcgcggc                    <  4:317224/141‑1 (MQ=255)
                                                                                                                               gACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTaccac           >  4:247854/1‑149 (MQ=255)
                                                                                                                                acacCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCTAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTaccacc          >  5:126628/1‑149 (MQ=255)
                                                                                                                                acacCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGcc                                                 >  7:31604/1‑110 (MQ=255)
                                                                                                                                acacCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGcc                                                 <  8:31604/110‑1 (MQ=255)
                                                                                                                                acacCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGt                  >  7:248407/1‑141 (MQ=255)
                                                                                                                                acacCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGt                  <  8:248407/141‑1 (MQ=255)
                                                                                                                                acacCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTtccacc          >  1:226846/1‑149 (MQ=255)
                                                                                                                                  acCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCgg        <  7:39940/149‑1 (MQ=255)
                                                                                                                                  acCGACATGTTCCACCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCgg        <  8:316325/149‑1 (MQ=255)
                                                                                                                                    cGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCgg        >  2:166765/1‑147 (MQ=255)
                                                                                                                                    cGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCgg        <  1:166765/147‑1 (MQ=255)
                                                                                                                                    cGACATGTTCCACCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCtgtg                                   >  1:90967/1‑120 (MQ=255)
                                                                                                                                    cGACATGTTCCACCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCtgtg                                   <  2:90967/120‑1 (MQ=255)
                                                                                                                                       cATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCGGGCAtt   <  4:282017/149‑1 (MQ=255)
                                                                                                                                        aTGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCTCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCGGGCATTg  <  6:286538/149‑1 (MQ=255)
                                                                                                                                        aTGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCt                                            <  2:77453/107‑1 (MQ=255)
                                                                                                                                        aTGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCt                                            >  1:77453/1‑107 (MQ=255)
                                                                                                                                           ttCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCtgt                                    >  1:308326/1‑112 (MQ=255)
                                                                                                                                           ttCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCtgt                                    <  2:308326/112‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                             
AGGGGCGGCGCCAAAGATGAGCATCTACAGCGCCAGCAAGCATGCGGTCATCGGTCTGACCAAGTCGGCGGCCATCGAGTACGCCAAGAAGGGCATCCGCGTGAACGCCGTGTGCCCGGCCGTGATCGACACCGACATGTTCCGCCGCGCTTACCAGGCCGACCCGCGCAAGGCCGAGTTCGCCGCAGCCATGCACCCGGTAGGGCGCATTGGCAAGGTCGAGGAAATCGCCAGCGCCGTGCTGTATCTGTGCAGTGACGGCGCGGCGTTTACCACCGGGCATTG  >  NC_002947/2043176‑2043460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑