Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 132,467 G→C 9.4% intergenic (+41/‑111) cc → / → dsbA cytochrome c4/thiol:disulfide interchange protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947132,4670GC 9.4% 135.9 / 4.9 51intergenic (+41/‑111)cc/dsbAcytochrome c4/thiol:disulfide interchange protein
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base C (3/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01

AACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGGTCGCCGC  >  NC_002947/132322‑132615
                                                                                                                                                 |                                                                                                                                                    
aaCCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGc                                                                                                                                                   >  5:19104/1‑149 (MQ=255)
      aCCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCt                                                                                                                                             >  7:23212/1‑149 (MQ=255)
         aaCGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTCGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCtttt                                                                                                                                          <  6:345486/149‑1 (MQ=255)
             aTGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCtttcatca                                                                                                                                      <  5:19718/149‑6 (MQ=255)
               ggCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgt                                                                                                                                    >  5:203887/1‑149 (MQ=255)
               ggCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgt                                                                                                                                    >  8:145716/1‑149 (MQ=255)
                gCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgtc                                                                                                                                   <  4:352391/149‑1 (MQ=255)
                       cATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGGTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGACCTTTTTTCCGTCCGCAGcc                                                                                                                            >  4:341044/1‑149 (MQ=255)
                       cATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGGTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCGTTTTTTCCGTCCGCAGcc                                                                                                                            >  4:42645/1‑149 (MQ=255)
                            ccATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTac                                                                                                                       <  6:6708/149‑1 (MQ=255)
                             cATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTaca                                                                                                                      >  7:214508/1‑149 (MQ=255)
                                    aCCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTACCGTCCGCAGCCGTTACACTACAg                                                                                                                >  7:319466/1‑148 (MQ=255)
                                     ccATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGaa                                                                                                              <  6:284239/149‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgtc                                                                                                                                   >  2:338962/1‑119 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTtccgtc                                                                                                                                   <  1:338962/119‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGc                                                                                                       <  8:264408/147‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGc                                                                                                       <  6:47684/147‑1 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCtttc                                                                                                                                          <  7:325336/112‑2 (MQ=255)
                                              gCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCAGCCCGGCCGCCCtttc                                                                                                                                          >  8:325336/1‑111 (MQ=255)
                                                      aGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCg                                                                                               <  6:19104/147‑1 (MQ=255)
                                                        cAACCATGACATCGAAGCGTAGGCCAGCTACATCCAGGGCCTGCAATAACTTTCGGTTAAAGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTCCCCTTTTTTCCATACGCAGCCGTTAAACTACAGAACACCAGCCCTTCCAGGcat                                                                                           >  3:5076/1‑147 (MQ=255)
                                                             aTGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGtct                                                                                       >  1:99721/1‑148 (MQ=255)
                                                              tGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGa                                                                                                         >  2:201197/1‑129 (MQ=255)
                                                              tGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCCTCCGCAGCCGTTACACTACAGAACTCGa                                                                                                         <  1:201197/129‑1 (MQ=255)
                                                                aCATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGt                                                                                   >  1:94804/1‑149 (MQ=255)
                                                                  aTCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGc                                                                                 <  3:243940/149‑1 (MQ=255)
                                                                   tCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTg                                                                                  >  3:333093/1‑147 (MQ=255)
                                                                     gAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAAGTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCAGCAGCCGTTACACTACAGAACTCGCGCCATTCCCGCCCTTTCTCAGTGCAgg                                                                              >  3:264066/1‑149 (MQ=255)
                                                                     gAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAgg                                                                              >  6:164306/1‑149 (MQ=255)
                                                                       aGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTc                                                                            <  6:203887/149‑1 (MQ=255)
                                                                              ggaaGCTTCATCCAGGGGCTGCATTAACTTTCGTTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGcgtcgt                                                                     <  4:5076/146‑1 (MQ=255)
                                                                                      cATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGAcc                                                              >  1:216409/1‑148 (MQ=255)
                                                                                        tCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCAt                                                           <  2:94804/149‑1 (MQ=255)
                                                                                                   cATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAg                                                 >  7:107245/1‑148 (MQ=255)
                                                                                                   cATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGATACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAg                                                 >  3:142440/1‑148 (MQ=255)
                                                                                                       aaCTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTACCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGt                                             <  8:319466/148‑1 (MQ=255)
                                                                                                         cTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTaaa                                          <  8:214508/149‑1 (MQ=255)
                                                                                                          tttCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCGTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAg                                         >  5:265503/1‑149 (MQ=255)
                                                                                                             cGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCa                                       <  7:145716/148‑1 (MQ=255)
                                                                                                                  aaTGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGt                                  >  4:264569/1‑148 (MQ=255)
                                                                                                                   aTGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTaa                                <  6:265503/149‑1 (MQ=255)
                                                                                                                   aTGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTa                                 >  1:296341/1‑148 (MQ=255)
                                                                                                                         tAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGAt                          >  3:274620/1‑149 (MQ=255)
                                                                                                                             tCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATtctc                      <  2:99721/149‑1 (MQ=255)
                                                                                                                                   aaaaaaGGGCAGCCCGGCCGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAgcgct                >  4:143020/4‑149 (MQ=255)
                                                                                                                                    aaaaaGGGCAGCCCGGCCGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAgcgctg               >  4:44626/3‑149 (MQ=255)
                                                                                                                                     gaAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAgcgctgc              >  7:40126/1‑149 (MQ=255)
                                                                                                                                         gggCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGcgctgcgctg          <  8:40126/149‑1 (MQ=255)
                                                                                                                                          ggcggcCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTgg         <  2:296341/149‑1 (MQ=255)
                                                                                                                                             ggcCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAAc                              <  3:240061/125‑1 (MQ=255)
                                                                                                                                             ggcCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAAc                              >  4:240061/1‑125 (MQ=255)
                                                                                                                                                 gggCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGGTcgccgc  <  8:23212/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AACCGCACCAACGATGGCGATGCCATGACCATGCGCACCATCGCTGGCAAGCTGAGCAACCATGACATCGAAGCGTTGGCCAGCTACATCCAGGGCCTGCATTAACTTTCGGTTAATGTTGTAGGTCAATGATGAAAGGGCGGCCGGGCTGCCCTTTTTTCCGTCCGCAGCCGTTACACTACAGAACTCGAGCCCTTCCCGGCCTGTCTCAGTGCAGGTCGCGTCGTGGCGACCCATGACTGCTCAGGAGTAAAGCATGCGTAAACTGATTCTCAGCGCTGCGCTGGTCGCCGC  >  NC_002947/132322‑132615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑