| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,057,851 | G→A | 12.1% | intergenic (+67/+61) | PP_1832 → / ← PP_1833 | oxidase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,057,851 | 0 | G | A | 12.1% | 81.4 / 3.1 | 33 | intergenic (+67/+61) | PP_1832/PP_1833 | oxidase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base A (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
GTATGGCAGCGAGCAGGGCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCACTGGCCTCTTCGGCCAGGT > NC_002947/2057703‑2057994 | gTATGGCAGCGAGCAGGGCATGCAGGCGCGGATTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAAACCGCGACGAAAATGACTTAGCGATTCCGAAGGgcggcg > 3:97183/1‑149 (MQ=255) aGCGAGCTGGGCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCgt < 5:102146/149‑1 (MQ=255) gAGCAGGGCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCACAACCgtcgg > 3:288707/1‑149 (MQ=255) gCAGGGCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCgtcggtc < 2:42817/149‑1 (MQ=255) gggCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTa < 1:145187/149‑1 (MQ=255) gggCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTa < 7:307155/149‑1 (MQ=255) gggCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGGGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTa < 8:260658/149‑1 (MQ=255) aTGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAg > 3:119899/1‑149 (MQ=255) cAGGCGCGGTTTACTGGCGATGGTAGTGCGCGGGCATTTGTTGGTATCGACGAGCTGTAATCGCATTGCCGGCGGCTTGGCGGGGGAAGACGCTCCTACATTGACTGCGCGATGGCTAAGGGCGGCGCAACAGTCGGTCGTAGGAGCg < 4:200572/148‑1 (MQ=255) cAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCgg < 6:147034/149‑1 (MQ=255) gcgGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCa < 4:119899/149‑1 (MQ=255) gTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCACAACCGTCGGTCGTAGGAGCGGGTTCAccc < 4:288707/149‑1 (MQ=255) gggCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAAACCCCTCCGACATTTACTGCGCGCTGCCGAAGGGCGGGGCAACCGGCGGGGGTAGGAGCGGGGTGACCcgcg > 3:303361/1‑146 (MQ=255) gACTGCGCGGGCATTTCTTGGCATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGtt > 3:84181/1‑129 (MQ=255) gACTGCGCGGGCATTTCTTGGCATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGtt < 4:84181/129‑1 (MQ=255) tCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCGCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACAccc < 7:268909/148‑1 (MQ=255) tGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCCCTCCTTCATTGACTGCGCGTTGCCTAAGGGCGGCGCAACCGTCGGTCGGAGGAGCGGGTTCACCCCCGAAGAGGCCAGTTGAGGCAATACAccccggc > 5:345493/1‑146 (MQ=255) gacgaGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTa < 4:303361/148‑1 (MQ=255) acgaGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCAGCGaa < 1:117566/113‑1 (MQ=255) acgaGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCCGCGCAAACGTCGGTCGTAGGAGCGGGTTCACCAGAGaa > 2:117566/1‑113 (MQ=39) cgGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTg > 8:124990/1‑146 (MQ=255) cgGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCACAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCaaa > 2:220945/1‑131 (MQ=255) cgGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCACAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCaaa < 1:220945/131‑1 (MQ=255) gTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCAt > 4:11628/1‑149 (MQ=255) aCCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATc > 4:18169/1‑146 (MQ=255) gCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGa > 8:49222/1‑149 (MQ=255) cTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGAc < 3:18169/149‑1 (MQ=255) aTTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAAt > 3:288336/1‑149 (MQ=255) aCTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCa < 4:288336/148‑1 (MQ=255) cgggATGCATAAGGGCGGGGCAACCTTCGGTCGTAGGAGGGGGTTAACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCACTg < 3:296535/144‑1 (MQ=255) tGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCACTGGCCTCtt > 8:51749/1‑149 (MQ=255) cTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGAAATTCACTTGCCTCTTCgg > 1:279048/1‑149 (MQ=255) tAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCACTGGCCTCTTCGGc > 7:314818/1‑149 (MQ=255) ggcggcGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCACTGGCCTCTTCGGCCAgg > 8:111628/1‑149 (MQ=255) gcggcgCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCACTGGCCTCTTCGGCCAGGt > 6:311987/1‑149 (MQ=255) | GTATGGCAGCGAGCAGGGCATGCAGGCGCGGTTTACGGGCGATGTGACTGCGCGGGCATTTCTTGGTATCGACGAGCTGTAATCGCATTGCCGGCCGCTTCGCGGGTGAACCCGCTCCTACATTGACTGCGCGATGCCTAAGGGCGGCGCAACCGTCGGTCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGTTCAGGCAATACACCCCTCAGCGGTAGGTCGGCAAGGCAAACCGCTGCTGGCTCTGCAGCATCGAGATGACCGGCAATTCACTGGCCTCTTCGGCCAGGT > NC_002947/2057703‑2057994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |