| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,084,112 | T→A | 9.4% | intergenic (+16/+13) | PP_1862 → / ← PP_1863 | membrane protein/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,084,112 | 0 | T | A | 9.4% | 149.2 / 5.8 | 53 | intergenic (+16/+13) | PP_1862/PP_1863 | membrane protein/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (23/25); new base A (2/3); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01 | |||||||||||
CTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGAT > NC_002947/2083964‑2084256 | cTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTTCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGAtt > 5:351197/1‑149 (MQ=255)cTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGAtt > 6:325507/1‑149 (MQ=255) gCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCg < 7:98519/149‑1 (MQ=255) ggtggCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGAAAATCGGCCCCa < 7:64253/149‑1 (MQ=255) gCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGAt > 1:117031/1‑148 (MQ=255) ggtCAGCATGAGCAGGGGTGTGAAGCTGGCCCCAGCGTTGGCGGCGCAGGGATTGGGGGCGAGGTTGTGTGTGTTCCTGGTGGTGTGTGGGGGGATGCTGGCGTTTGAGCTTTGAGATTTTTGGGGCGATTTTAGGCCCCAGAGCAc > 2:143804/3‑147 (MQ=255) gCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACtt > 2:348426/1‑149 (MQ=255) tCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACtt > 8:328391/1‑147 (MQ=255) tCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACtt < 7:328391/147‑1 (MQ=255) cATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCgg < 7:106581/130‑1 (MQ=255) cATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCgg > 8:106581/1‑130 (MQ=255) cATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAAcc < 5:30384/149‑1 (MQ=255) cTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGAAAATCGGccc < 3:75754/127‑1 (MQ=255) cTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGAAAATCGGccc > 4:75754/1‑127 (MQ=255) gggTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGAt < 6:351197/149‑1 (MQ=255) ggTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATg < 5:325507/149‑1 (MQ=255) gtgtGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTATGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGt < 6:318328/149‑1 (MQ=255) aaGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTc < 2:117031/148‑1 (MQ=255) cTGGCCCATGCGTTTCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGcc < 3:72896/149‑1 (MQ=255) gCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCAt < 6:33566/148‑1 (MQ=255) gCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCAt < 1:143804/148‑1 (MQ=255) ccATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATg > 6:45718/1‑147 (MQ=255) ccATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTAGAAACCCTCAACGATGTAGTCGGCCATg > 2:122268/1‑147 (MQ=255) cggtGCCGGAGAAGGTATTGCCGGCGAGGTTCTGTGTGGTCCTGGTGGGCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCgg < 8:250212/146‑1 (MQ=255) ttGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTg < 1:348426/149‑1 (MQ=255) ggTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAggg > 2:121000/1‑149 (MQ=255) ttGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAgg < 1:221683/144‑1 (MQ=255) ttGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAgg > 2:221683/1‑144 (MQ=255) cGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTc < 8:52437/111‑1 (MQ=255) cTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCgg > 1:113971/1‑118 (MQ=255) cTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCgg < 2:113971/118‑1 (MQ=255) cTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCgg > 8:279889/1‑108 (MQ=255) cTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCgg < 7:279889/108‑1 (MQ=255) gtggtCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTg < 8:173332/149‑1 (MQ=255) gtCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGc < 5:45718/148‑1 (MQ=255) tgCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATggg > 8:158962/1‑149 (MQ=255) tgCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCAGTACATTGGCCATggg > 7:307318/1‑149 (MQ=255) cGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGc < 7:158962/148‑1 (MQ=255) ggTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATg < 3:278306/75‑1 (MQ=255) ggTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATg > 4:278306/1‑75 (MQ=255) gCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCg > 8:309694/1‑97 (MQ=255) gCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCg < 7:309694/97‑1 (MQ=255) tGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCgg > 6:351030/1‑147 (MQ=255) tttGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGctct > 7:162568/1‑149 (MQ=255) gaTTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCAcc > 8:90553/1‑148 (MQ=255) gaTTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCAcc > 7:190334/1‑148 (MQ=255) ttGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGaa > 2:66099/1‑149 (MQ=255) ctGGGGCCGAAAATCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTg < 2:116776/108‑1 (MQ=255) ctGGGGCCGAAAATCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTg > 1:116776/2‑109 (MQ=255) gCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGc < 4:77405/119‑1 (MQ=255) gCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGc > 3:77405/1‑119 (MQ=255) ccGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGa < 5:351030/149‑1 (MQ=255) ccGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTAATGCGCAAAAGCATTACATTGGCCATGGGCAACTGCGGCAGCCGTTCGCTCTGACCCAGAATGCGGa > 6:131299/1‑149 (MQ=255) cGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGACATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGAt > 7:169983/1‑149 (MQ=255) cGATTTTCGGCCCCAGATCACTAGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGAt < 1:122268/149‑1 (MQ=255) | CTTGGCGGTGGGTGGCTTGCTCAGCATCAGCTGGGGTGTGAAGCTGGCCCATGCGTTGCCGGAGAAGGTATTGCGGGCGATGTTCTGTGTGTTCCTGGTGGTCTGTGCGGTGATGCTGGCGTTTGAGCTTTGAGATTTTGGGGCCGATTTTCGGCCCCAGATCACTTGAAACCCTCAACGATGTAGTCGGCCATGCAGTCGGTGATTGGCGAGGGGGTCTGCGTACTGCGCACCAGCATTACATTGGCCATGGGCAACTGCGGCAGCCCTTCGCTCTCACCCAGAATGCGGAT > NC_002947/2083964‑2084256 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |