Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	142,172	T→C	5.8%	L39S (TTG→TCG)	PP_0134 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	142,172	0	T	C	5.8%	205.8 / 2.1	69	L39S (TTG→TCG)	PP_0134	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/34);  new base C (2/2);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TTTTCAGATTTTGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAG  >  NC_002947/142024‑142318                                                                                                                                                     |                                                                                                                                                   ttttCAGATTTTGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCtt                                                                                                                                                    <  3:313654/149‑1 (MQ=255) ttttCAGATGTTGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCtt                                                                                                                                                    <  7:149298/149‑1 (MQ=255)    tCAGATTTTGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGt                                                                                                                                                  >  8:223495/1‑148 (MQ=255)      aGATTTTGCCCACGTAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCa                                                                                                                                                <  5:296029/148‑1 (MQ=255)      aGATTTTGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCa                                                                                                                                                <  7:154101/148‑1 (MQ=255)      aGATTTTGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCa                                                                                                                                                <  5:337685/148‑1 (MQ=255)           ttGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTCGTCACTGAAc                                                                                                                                          >  3:18993/1‑149 (MQ=255)                cACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCa                                                                                                                                                <  7:137822/138‑1 (MQ=255)                cACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCa                                                                                                                                                >  8:137822/1‑138 (MQ=255)                 aCGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGt                                                                                                                                                  <  6:176404/135‑1 (MQ=255)                 aCGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGt                                                                                                                                                  >  5:176404/1‑135 (MQ=255)                 aCGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATg                                                                                                                                    <  2:300815/149‑1 (MQ=255)                  cGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCa                                                                                                                                                <  7:308880/136‑1 (MQ=255)                  cGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCa                                                                                                                                                >  8:308880/1‑136 (MQ=255)                   gAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCt                                                                                                                                  >  3:180835/1‑149 (MQ=255)                      ggaggCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGa                                                                                                                               >  1:177202/1‑149 (MQ=255)                                tCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCg                                                                                                                      <  4:180835/148‑1 (MQ=255)                                tCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGA‑CCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCa                                                                                                                    >  5:287466/1‑149 (MQ=255)                                     cccTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATc                                                                                                                  >  2:106140/1‑147 (MQ=255)                                       cTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAgg                                                                                                              >  7:33688/1‑149 (MQ=255)                                        ttttCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGt                                                                                                             >  5:157148/1‑149 (MQ=255)                                             ccTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTCGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGaa                                                                                                         <  4:18993/148‑1 (MQ=255)                                                  gTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGAc                                                                                                   <  2:177202/149‑1 (MQ=255)                                                                gccgccACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCAtt                                                                                                                         <  5:304566/113‑1 (MQ=255)                                                                gccgccACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCAtt                                                                                                                         >  6:304566/1‑113 (MQ=255)                                                                 ccgccACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCgg                                                                                    >  1:265244/1‑149 (MQ=255)                                                                  cgccACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCtt                                                                                                                                 >  7:30101/1‑103 (MQ=255)                                                                  cgccACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCtt                                                                                                                                 <  8:30101/103‑1 (MQ=255)                                                                  cgccACCCTGCAGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGCCGCGGc                                                                                   <  3:254998/149‑1 (MQ=255)                                                                   gccACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCt                                                                                  >  3:78576/1‑149 (MQ=255)                                                                             tgcCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTCGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTg                                                                                                           >  4:258063/1‑114 (MQ=255)                                                                             tgcCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTCGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTg                                                                                                           <  3:258063/114‑1 (MQ=255)                                                                              gcCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCgg                                                                                    >  7:160909/1‑136 (MQ=255)                                                                              gcCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCgg                                                                                    <  8:160909/136‑1 (MQ=255)                                                                               ccAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGt                                                                      >  5:137285/1‑149 (MQ=255)                                                                               caaaTCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGCCGCGGCTTTCCGGTGAgtt                                                                      >  1:116578/3‑147 (MQ=255)                                                                                cAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTg                                                                     <  6:157148/149‑1 (MQ=255)                                                                                cAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTg                                                                     <  8:33688/149‑1 (MQ=255)                                                                                cAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTg                                                                     <  5:169190/149‑1 (MQ=255)                                                                                 aaTCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTc                                                                                                                       >  3:204615/1‑98 (MQ=255)                                                                                 aaTCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTc                                                                                                                       <  4:204615/98‑1 (MQ=255)                                                                                  aTCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGga                                                                   <  6:142396/149‑1 (MQ=255)                                                                                        aCCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAAc                                                               >  8:320860/1‑147 (MQ=255)                                                                                        aCCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAAc                                                               <  7:320860/147‑1 (MQ=255)                                                                                         ccccGGCATTCGCCTACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAg                                                            <  8:114951/149‑1 (MQ=255)                                                                                         ccccGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAg                                                            >  5:71382/1‑149 (MQ=255)                                                                                          cccGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAgg                                                                                                              >  8:23237/1‑98 (MQ=255)                                                                                          cccGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAgg                                                                                                              <  7:23237/98‑1 (MQ=255)                                                                                              gCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGa                                                       <  3:344483/149‑1 (MQ=255)                                                                                                 ttCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCg                                                    >  5:301435/1‑149 (MQ=255)                                                                                                   cGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCgg                                                   <  1:106140/148‑1 (MQ=255)                                                                                                    gCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGa                                                                                                    >  6:38669/1‑98 (MQ=255)                                                                                                    gCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGa                                                                                                    <  5:38669/98‑1 (MQ=255)                                                                                                        aCCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCGTTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTg                                             >  7:8633/1‑149 (MQ=255)                                                                                                           cTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGAGTCATTTCGCATCAAGGTTGAAGTGGACGGCACGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGcc                                          >  8:143143/1‑149 (MQ=255)                                                                                                            tgtgCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGcc                                          >  1:16289/1‑148 (MQ=255)                                                                                                              tgCTGGACGGGAGGCTTGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGTACTGCAAGCAGGCGCGGCTTTCCGGTGATGTGGAGAACGAGGTCGAGCGGCAGGTGGCCGa                                        <  2:116578/148‑1 (MQ=255)                                                                                                              tgCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCg                                         <  4:78576/147‑1 (MQ=255)                                                                                                              tgCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAg                                       <  6:287466/149‑1 (MQ=255)                                                                                                                   gACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGt                                  <  6:301435/149‑1 (MQ=255)                                                                                                                     cgcgcgCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTgg                                >  1:299353/1‑149 (MQ=255)                                                                                                                        gcgcCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGccc                             >  4:239730/1‑149 (MQ=255)                                                                                                                        gcgcCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGccc                             >  6:14794/1‑149 (MQ=255)                                                                                                                         cgcCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCg                            >  8:259677/1‑149 (MQ=255)                                                                                                                            cTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGc                         >  2:71688/1‑149 (MQ=255)                                                                                                                                  ggCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCg                   <  2:265244/149‑1 (MQ=255)                                                                                                                                        cTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCAt             <  6:137285/149‑1 (MQ=255)                                                                                                                                               ccGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAg      <  1:71688/149‑1 (MQ=255)                                                                                                                                               ccGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAg      <  2:299353/149‑1 (MQ=255)                                                                                                                                                 gCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGt    <  2:16289/149‑1 (MQ=255)                                                                                                                                                   cTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTag  <  3:239730/149‑1 (MQ=255)                                                                                                                                                     tGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTa   >  6:174185/1‑146 (MQ=255)                                                                                                                                                     |                                                                                                                                                   TTTTCAGATTTTGCCCACGAAGGAGGCGCTGTCATGCCCTTTTCCCTGCGTACAGCCGTTTTGGCCGCCACCCTGCTGCCAATCTTTACCCCGGCATTCGCCGACCCTGTGCAGGACGCGCGCCTGGAAGGCGCCCTGCAAACCGCCTTGTCACTGAACCGCATGCTTGATTCATTTCGCATCAAGGTTGAAGTGGACGGCAAGCAGGCGCGGCTTTCCGGTGAGGTGGAGAACGAGGTCGAGCGGCAGCTGGCCGAGGACGTGGCCCGTGCTACCCGGGGCATCGAACAGGTAG  >  NC_002947/142024‑142318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑