Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,138,409	T→G	5.8%	L171R (CTG→CGG)	yadG →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,138,409	0	T	G	5.8%	201.6 / 2.0	64	L171R (CTG→CGG)	yadG	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (31/29);  new base G (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAG  >  NC_002947/2138261‑2138553                                                                                                                                                      |                                                                                                                                                 aCTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCt                                                                                                                                                  <  2:182626/149‑1 (MQ=255)   tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTgc                                                                                                                                                >  4:136477/1‑149 (MQ=255)   tCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCGgc                                                                                                                                                >  4:326918/1‑149 (MQ=255)     aGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTgcgc                                                                                                                                              >  6:29075/1‑149 (MQ=255)          ggCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGcgccgc                                                                                                                                           <  3:136477/147‑1 (MQ=255)             cTGTGGGACAAACGTTATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGTTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGAt                                                                                                                                      <  5:261749/149‑1 (MQ=255)             cTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGAt                                                                                                                                      >  6:253853/1‑149 (MQ=255)                tgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGAtgtg                                                                                                                                   <  2:235094/149‑1 (MQ=255)                tgGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAtatc                                                                                                                                   <  3:326918/149‑4 (MQ=255)                 gggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                  <  8:293667/149‑1 (MQ=255)                  ggACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                  <  8:207228/148‑1 (MQ=255)                       aaCGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTc                                                                                                                            <  5:12997/149‑1 (MQ=255)                        aCGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCGGCGCAGCTCGAta                                                                                                                                     <  7:283291/139‑2 (MQ=255)                                tgCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAg                                                                                                                   <  5:29075/149‑1 (MQ=255)                                  cAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGCACCGCGCCTGCTGATCCTGGACGA‑ACCCCCCGCCGGGGTGGATATCGAGCGGCGCCGCGCCAGGTGGAGCTTCCTCACGGCGCt                                                                                                                 >  6:214060/1‑149 (MQ=255)                                  cAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCt                                                                                                                 <  7:163658/149‑1 (MQ=255)                                           tcgttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAg                                                                                                          >  6:288887/1‑147 (MQ=255)                                            cgttGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGaa                                                                                                        <  5:288887/148‑1 (MQ=255)                                                        gtaTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACcat                                                                                            >  7:317364/3‑148 (MQ=255)                                                          aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                  <  4:12733/108‑1 (MQ=255)                                                          aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTgg                                                                                                                                  >  3:12733/1‑108 (MQ=255)                                                          aTGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCCCcatcat                                                                                         >  8:312278/1‑149 (MQ=255)                                                           tGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCatcatc                                                                                        >  8:323546/1‑149 (MQ=255)                                                                     ccTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCAccaccc                                                                              <  5:253853/149‑1 (MQ=255)                                                                      cTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCAGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCAGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCAccaccc                                                                              >  5:240273/1‑148 (MQ=255)                                                                         atgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCatcatc                                                                                        >  3:212075/1‑135 (MQ=255)                                                                         atgatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCatcatc                                                                                        <  4:212075/135‑1 (MQ=255)                                                                           gatTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATc                                                                        >  8:124338/1‑149 (MQ=255)                                                                               gCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTgg                                                                     >  2:213533/1‑148 (MQ=255)                                                                                       aTTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCt                                                             >  4:240146/1‑148 (MQ=255)                                                                                       aTTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCt                                                             >  7:246448/1‑148 (MQ=255)                                                                                           aTCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagca                                                        >  7:70038/1‑149 (MQ=255)                                                                                           aTCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagc                                                         <  7:323546/148‑1 (MQ=255)                                                                                             ccACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGagcagc                                                      <  7:312278/149‑1 (MQ=255)                                                                                              cACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCt                                                     <  7:124338/149‑1 (MQ=255)                                                                                              cACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCt                                                     >  8:150560/1‑149 (MQ=255)                                                                                              cACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCt                                                     <  8:246448/149‑1 (MQ=255)                                                                                               aCGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtg                                                    >  4:177940/1‑149 (MQ=255)                                                                                               aCGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtg                                                    >  4:172431/1‑149 (MQ=255)                                                                                                cGAACCGCGCCTGCTGATCCTGGACGATACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtg                                                    >  1:9972/1‑149 (MQ=255)                                                                                                 gAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCtgtg                                                  <  1:213533/149‑1 (MQ=255)                                                                                                   aCCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGcc                                                <  8:317364/149‑1 (MQ=255)                                                                                                     cgcgCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGc                                                              <  6:175202/133‑1 (MQ=255)                                                                                                     cgcgCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGc                                                              >  5:175202/1‑133 (MQ=255)                                                                                                      gcgcCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGt                                              >  6:24198/1‑148 (MQ=255)                                                                                                      gcgcCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGt                                              >  3:214841/1‑148 (MQ=255)                                                                                                             ctgATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCg                                       <  5:214060/148‑1 (MQ=255)                                                                                                                aTCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGcat                                   <  8:70038/149‑1 (MQ=255)                                                                                                                   cTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAAc                                                                                                             >  7:112531/1‑72 (MQ=255)                                                                                                                   cTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAAc                                                                                                             <  8:112531/72‑1 (MQ=255)                                                                                                                   cTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGcatcat                                >  7:252875/1‑149 (MQ=255)                                                                                                                   cTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGcatcat                                >  8:285059/1‑149 (MQ=255)                                                                                                                      gACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCg                              <  4:214841/148‑1 (MQ=255)                                                                                                                         gA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCa                          >  4:179832/1‑149 (MQ=255)                                                                                                                         gA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCa                          >  5:41073/1‑149 (MQ=255)                                                                                                                            aCCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCATCACTGAGCTGAACCAGAAGGGCATCACCATAATCATCACCACCGACTAACCGGAAGAGGCTGCGCAGCTGTGCCGTAACATCGGCATCATCGACCagg                        >  8:301562/1‑147 (MQ=255)                                                                                                                               cACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCa                     >  5:169014/1‑149 (MQ=255)                                                                                                                                aCCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGt                                              <  4:303831/123‑1 (MQ=255)                                                                                                                                aCCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGt                                              >  3:303831/1‑123 (MQ=255)                                                                                                                                       gggTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCAtcgtc             <  8:252875/149‑1 (MQ=255)                                                                                                                                         gTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCga           <  6:240273/149‑1 (MQ=255)                                                                                                                                         gTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCga           <  7:150560/149‑1 (MQ=255)                                                                                                                                             atatCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGaa        >  4:152061/1‑148 (MQ=255)                                                                                                                                                tCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACAcc    <  7:285059/149‑1 (MQ=255)                                                                                                                                                  gAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTCTCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAg  <  6:169014/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                 ACTCAGCTGGGCCTGTGGGACAAACGTGATGTGCAGTCGCGTTCGTTGTCCGGCGGCATGAAGCGGCGCCTGATGATTGCCCGCGCATTGATCCACGAACCGCGCCTGCTGATCCTGGACGA‑ACCCACCGCCGGGGTGGATATCGAGCTGCGCCGCTCGATGTGGAGCTTCCTCACTGAGCTGAACCAGAAGGGCATCACCATCATCCTCACCACCCACTATCTGGAAGAGGCTGAGCAGCTGTGCCGTAACATCGGCATCATCGACCATGGCACCATCGTCGAGAACACCAG  >  NC_002947/2138261‑2138553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑