Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,139,622	T→G	8.8%	Q167H (CAA→CAC)	PP_5481 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,139,622	0	T	G	8.8%	200.6 / 6.6	67	Q167H (CAA→CAC)	PP_5481	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/30);  new base G (2/4);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

CGCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACT  >  NC_002947/2139478‑2139767                                                                                                                                                 |                                                                                                                                                  cGCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGc                                                                                                                                               <  4:206009/149‑1 (MQ=255)    tACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcgac                                                                                                                                            >  5:30279/1‑149 (MQ=255)    tACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGATTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcgac                                                                                                                                            >  3:96741/1‑149 (MQ=255)      cGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcgacga                                                                                                                                          >  6:84896/1‑149 (MQ=255)         cATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGTCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGc                                                                                                                                       <  4:96741/149‑1 (MQ=255)         cATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGc                                                                                                                                       <  6:30279/149‑1 (MQ=255)               aGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGc                                                                                                                                 >  4:50155/1‑149 (MQ=255)                ggggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCaat                                                                                                                                   <  6:62816/146‑3 (MQ=255)                ggggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCAATGc                                                                                                                                 <  3:131501/148‑1 (MQ=255)                   gtgtCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGc                                                                                                                             >  1:168111/1‑149 (MQ=255)                           tatCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCccacccac                                                                                                                     <  3:50155/149‑1 (MQ=255)                            atCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCccacccac                                                                                                                     >  2:320638/1‑148 (MQ=255)                              cAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAg                                                                                                                  >  1:299063/1‑149 (MQ=255)                                gCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgcg                                                                                                                >  2:202008/1‑149 (MQ=255)                                 cATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgcgc                                                                                                               >  7:207173/1‑149 (MQ=255)                                      gCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGt                                                                                                           <  5:84896/148‑1 (MQ=255)                                         cGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGaa                                                                                                       <  2:299063/149‑1 (MQ=255)                                           gCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACa                                                                                                     <  1:71044/149‑1 (MQ=255)                                            cGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAt                                                                                                    >  1:278442/1‑149 (MQ=255)                                              aTCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtgg                                                                                                  <  1:320638/149‑1 (MQ=255)                                               tCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCgg                                                                                                                              >  6:369013/1‑120 (MQ=255)                                               tCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCgg                                                                                                                              <  5:369013/120‑1 (MQ=255)                                               tCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtggt                                                                                                 >  3:10370/1‑149 (MQ=255)                                                         gCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                       >  8:22939/1‑149 (MQ=255)                                                             gtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCa                                                                                    <  4:10370/148‑1 (MQ=255)                                                                gCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGc                                                                                                                                               >  3:248759/1‑86 (MQ=255)                                                                gCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGc                                                                                                                                               <  4:248759/86‑1 (MQ=255)                                                                  cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                       >  7:244633/1‑140 (MQ=255)                                                                  cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                       <  8:244633/140‑1 (MQ=255)                                                                               tACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCCCCACCGCCAGTAGAACATGCTGGAGAAGTAGGCAAGGATTCCCAGCACCAcga                                                                 >  8:284458/1‑148 (MQ=255)                                                                                 cGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAcgccg                                                               <  7:22939/149‑1 (MQ=255)                                                                                   ttgttTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGca                                                             >  7:8377/1‑149 (MQ=255)                                                                                     gttTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGcaca                                                           >  8:185895/1‑149 (MQ=255)                                                                                         gCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGc                                                                                                                                 <  4:254934/75‑1 (MQ=255)                                                                                         gCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGc                                                                                                                                 >  3:254934/1‑75 (MQ=255)                                                                                             tCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgag                                                   >  4:210391/1‑149 (MQ=255)                                                                                             tCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgag                                                   >  1:140780/1‑149 (MQ=255)                                                                                               gCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagc                                                  <  7:185895/148‑1 (MQ=255)                                                                                                   gCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGccc                                                                           <  4:91155/119‑1 (MQ=255)                                                                                                   gCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGccc                                                                           >  3:91155/1‑119 (MQ=255)                                                                                                    cTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACcgagccgagc                                             <  8:207173/148‑1 (MQ=255)                                                                                                     tGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAg                                           >  4:319811/1‑149 (MQ=255)                                                                                                       gTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAg                                           <  2:278442/147‑1 (MQ=255)                                                                                                          aGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAgg                                      >  7:279424/1‑149 (MQ=255)                                                                                                           gCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAggg                                     <  2:301457/149‑1 (MQ=255)                                                                                                              gCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTg                                  >  6:314972/1‑149 (MQ=255)                                                                                                              agcgcgGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGtt                                   <  4:49915/146‑1 (MQ=255)                                                                                                                cgcgGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                 >  7:178631/1‑148 (MQ=255)                                                                                                                   ggCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCcaca                             <  8:8377/149‑1 (MQ=255)                                                                                                                   ggCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCca                               >  7:3382/1‑147 (MQ=255)                                                                                                                       tGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGt                         <  6:141110/149‑1 (MQ=255)                                                                                                                         cGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTc                        <  7:284458/148‑1 (MQ=255)                                                                                                                             ccTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAg                   >  8:262601/1‑149 (MQ=255)                                                                                                                              cTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCGGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGc                  <  1:202008/149‑1 (MQ=255)                                                                                                                              cTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGc                  <  3:319811/149‑1 (MQ=255)                                                                                                                                 aaGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGtt                                   <  4:157496/129‑1 (MQ=255)                                                                                                                                 aaGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGtt                                   >  3:157496/1‑129 (MQ=255)                                                                                                                                 aaGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCt                 <  5:314972/147‑1 (MQ=255)                                                                                                                                 aaGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTgg               >  2:185667/1‑149 (MQ=255)                                                                                                                                         ggccGGCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa        <  7:211807/146‑1 (MQ=255)                                                                                                                                         ggccGGCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa        >  8:211807/3‑148 (MQ=255)                                                                                                                                         cACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa        <  8:279424/148‑1 (MQ=255)                                                                                                                                            cGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccga                                               <  3:257062/106‑1 (MQ=255)                                                                                                                                            cGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccga                                               >  4:257062/1‑106 (MQ=255)                                                                                                                                             ggCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                 <  5:17926/119‑1 (MQ=255)                                                                                                                                             ggCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGc                                 >  6:17926/1‑119 (MQ=255)                                                                                                                                              gCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACt  <  7:262601/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CGCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACT  >  NC_002947/2139478‑2139767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑